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DRX081796: Illumina HiSeq 2500 paired end sequencing of SAMD00074919
1 ILLUMINA (Illumina HiSeq 2500) run: 26.1M spots, 5.2G bases, 2.4Gb downloads

Submitted by: TOTTORI
Study: Hordeum vulgare Transcriptome or Gene expression
show Abstracthide Abstract
Total RNA was extracted from immature spikes at the awn primordium stage of cultivated barley (Hordeum vulgare L.). Total RNA was measured by using Agilent 2100 Bioanalyzer (Agilent Technologies, Technologies, PaloAlto, CA, USA) and used for construction of sequencing library. Strand-specific RNA libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the instructions in the TruSeq Stranded mRNA Sample Preparation Guide Rev.E (Illumina). Poly(A)-RNA was fragmented and single-strand and double-strand cDNA were synthesized. High-throughput sequencing was conducted using HiSeq 2500 (Illumina, San Diego, CA, USA).
Sample: Foma_AP3
SAMD00074919 • DRS056029 • All experiments • All runs
Organism: Hordeum vulgare
Name: PR0443_03A06_H1
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Selection: PolyA
Layout: PAIRED
Construction protocol:
Spot descriptor:
forward101  reverse

Runs: 1 run, 26.1M spots, 5.2G bases, 2.4Gb
Run# of Spots# of BasesSizePublished


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