Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Lessard DV, Zinder OJ, Hotta T, Verhey KJ, Ryoma O, Berger CL. Regulation of KIF1A motility via polyglutamylation of tubulin C-terminal tails. Journal of Biol. Chem. Forthcoming. doi: 10.1101/410860.
Yue Y, Hotta T, Higaki T, Verhey KJ, Ohi R. Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice. Curr Biol. 2023 Sep 5;. doi: 10.1016/j.cub.2023.07.062. [Epub ahead of print] PubMed PMID: 37716348; NIHMSID:NIHMS1923163.
Verhey KJ, Ohi R. Causes, costs and consequences of kinesin motors communicating through the microtubule lattice. J Cell Sci. 2023 Mar 1;136(5). doi: 10.1242/jcs.260735. Epub 2023 Mar 3. PubMed PMID: 36866642; PubMed Central PMCID: PMC10022682.
Hotta T, Plemmons A, Gebbie M, Ziehm TA, Blasius TL, Johnson C, Verhey KJ, Pearring JN, Ohi R. Mechanistic Analysis of CCP1 in Generating ΔC2 α-Tubulin in Mammalian Cells and Photoreceptor Neurons. Biomolecules. 2023 Feb 12;13(2). doi: 10.3390/biom13020357. PubMed PMID: 36830726; PubMed Central PMCID: PMC9952995.
Solon AL, Zaniewski TM, O'Brien P, Clasby M, Hancock WO, Ohi R. Synergy between inhibitors of two mitotic spindle assembly motors undermines an adaptive response. Mol Biol Cell. 2022 Dec 1;33(14):ar132. doi: 10.1091/mbc.E22-06-0225. Epub 2022 Oct 6. PubMed PMID: 36200902; PubMed Central PMCID: PMC9727797.
Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R. EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Curr Biol. 2022 Sep 26;32(18):3898-3910.e14. doi: 10.1016/j.cub.2022.07.027. Epub 2022 Aug 12. PubMed PMID: 35963242; PubMed Central PMCID: PMC9530018.
Mathias TJ, Ju JA, Lee RM, Thompson KN, Mull ML, Annis DA, Chang KT, Ory EC, Stemberger MB, Hotta T, Ohi R, Vitolo MI, Moutin MJ, Martin SS. Tubulin Carboxypeptidase Activity Promotes Focal Gelatin Degradation in Breast Tumor Cells and Induces Apoptosis in Breast Epithelial Cells That Is Overcome by Oncogenic Signaling. Cancers (Basel). 2022 Mar 28;14(7). doi: 10.3390/cancers14071707. PubMed PMID: 35406479; PubMed Central PMCID: PMC8996877.
Solon A, Ohi R. Chemical Biology of Mitotic Spindle Assembly Motors. Methods Mol Biol. 2022;2415:151-165. doi: 10.1007/978-1-0716-1904-9_11. PubMed PMID: 34972952; NIHMSID:NIHMS1828833.
Begley MA, Solon AL, Davis EM, Sherrill MG, Ohi R, Elting MW. K-fiber bundles in the mitotic spindle are mechanically reinforced by Kif15. Mol Biol Cell. 2021 Dec 1;32(22):br11. doi: 10.1091/mbc.E20-06-0426. Epub 2021 Oct 20. PubMed PMID: 34668719; PubMed Central PMCID: PMC8694074.
Solon AL, Tan Z, Schutt KL, Jepsen L, Haynes SE, Nesvizhskii AI, Sept D, Stumpff J, Ohi R, Cianfrocco MA. Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding. Sci Adv. 2021 Nov 19;7(47):eabj9812. doi: 10.1126/sciadv.abj9812. Epub 2021 Nov 19. PubMed PMID: 34797717; PubMed Central PMCID: PMC8604404.
Hotta T, Haynes SE, Blasius TL, Gebbie M, Eberhardt EL, Sept D, Cianfrocco M, Verhey KJ, Nesvizhskii AI, Ohi R. Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin. Curr Biol. 2021 Feb 22;31(4):900-907.e6. doi: 10.1016/j.cub.2020.11.055. Epub 2021 Jan 21. PubMed PMID: 33482110; PubMed Central PMCID: PMC7931505.
Ohi R, Strothman C, Zanic M. Impact of the 'tubulin economy' on the formation and function of the microtubule cytoskeleton. Curr Opin Cell Biol. 2021 Feb;68:81-89. doi: 10.1016/ Epub 2020 Nov 4. Review. PubMed PMID: 33160109; PubMed Central PMCID: PMC7925340.
Ferguson FM, Nabet B, Raghavan S, Liu Y, Leggett AL, Kuljanin M, Kalekar RL, Yang A, He S, Wang J, Ng RWS, Sulahian R, Li L, Poulin EJ, Huang L, Koren J, Dieguez-Martinez N, Espinosa S, Zeng Z, Corona CR, Vasta JD, Ohi R, Sim T, Kim ND, Harshbarger W, Lizcano JM, Robers MB, Muthaswamy S, Lin CY, Look AT, Haigis KM, Mancias JD, Wolpin BM, Aguirre AJ, Hahn WC, Westover KD, Gray NS. Discovery of a selective inhibitor of doublecortin like kinase 1. Nat Chem Biol. 2020 Jun;16(6):635-643. doi: 10.1038/s41589-020-0506-0. Epub 2020 Apr 6. PubMed PMID: 32251410; PubMed Central PMCID: PMC7246176.
Taneja N, Bersi MR, Baillargeon SM, Fenix AM, Cooper JA, Ohi R, Gama V, Merryman WD, Burnette DT. Precise Tuning of Cortical Contractility Regulates Cell Shape during Cytokinesis. Cell Rep. 2020 Apr 7;31(1):107477. doi: 10.1016/j.celrep.2020.03.041. PubMed PMID: 32268086; PubMed Central PMCID: PMC8223100.
Strothman C, Farmer V, Arpağ G, Rodgers N, Podolski M, Norris S, Ohi R, Zanic M. Microtubule minus-end stability is dictated by the tubulin off-rate. J Cell Biol. 2019 Sep 2;218(9):2841-2853. doi: 10.1083/jcb.201905019. Epub 2019 Aug 16. PubMed PMID: 31420452; PubMed Central PMCID: PMC6719460.
Lessard DV, Zinder OJ, Hotta T, Verhey KJ, Ohi R, Berger CL. Polyglutamylation of tubulin's C-terminal tail controls pausing and motility of kinesin-3 family member KIF1A. J Biol Chem. 2019 Apr 19;294(16):6353-6363. doi: 10.1074/jbc.RA118.005765. Epub 2019 Feb 15. PubMed PMID: 30770469; PubMed Central PMCID: PMC6484136.
Malaby HLH, Dumas ME, Ohi R, Stumpff J. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol. 2019 Apr 1;218(4):1218-1234. doi: 10.1083/jcb.201806195. Epub 2019 Feb 1. PubMed PMID: 30709852; PubMed Central PMCID: PMC6446846.
Fonseca CL, Malaby HLH, Sepaniac LA, Martin W, Byers C, Czechanski A, Messinger D, Tang M, Ohi R, Reinholdt LG, Stumpff J. Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase. J Cell Biol. 2019 Apr 1;218(4):1148-1163. doi: 10.1083/jcb.201807228. Epub 2019 Feb 7. PubMed PMID: 30733233; PubMed Central PMCID: PMC6446859.
Dumas ME, Chen GY, Kendrick ND, Xu G, Larsen SD, Jana S, Waterson AG, Bauer JA, Hancock W, Sulikowski GA, Ohi R. Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes. Bioorg Med Chem Lett. 2019 Jan 15;29(2):148-154. doi: 10.1016/j.bmcl.2018.12.008. Epub 2018 Dec 4. PubMed PMID: 30528696; PubMed Central PMCID: PMC6681659.
Ohi R, Verhey KJ. Promoting Diversity in the Cytoskeleton through STEM-Structural Transitions and Energetics of Microtubule Subunits. Dev Cell. 2018 Oct 22;47(2):137-139. doi: 10.1016/j.devcel.2018.10.008. PubMed PMID: 30352173; PubMed Central PMCID: PMC6396305.
Reinemann DN, Norris SR, Ohi R, Lang MJ. Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor Traffic. Curr Biol. 2018 Jul 23;28(14):2356-2362.e5. doi: 10.1016/j.cub.2018.06.055. Epub 2018 Jul 12. PubMed PMID: 30017484.
Norris SR, Jung S, Singh P, Strothman CE, Erwin AL, Ohi MD, Zanic M, Ohi R. Microtubule minus-end aster organization is driven by processive HSET-tubulin clusters. Nat Commun. 2018 Jul 9;9(1):2659. doi: 10.1038/s41467-018-04991-2. PubMed PMID: 29985404; PubMed Central PMCID: PMC6037785.
Chiang YC, Park IY, Terzo EA, Tripathi DN, Mason FM, Fahey CC, Karki M, Shuster CB, Sohn BH, Chowdhury P, Powell RT, Ohi R, Tsai YS, de Cubas AA, Khan A, Davis IJ, Strahl BD, Parker JS, Dere R, Walker CL, Rathmell WK. SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell Carcinoma. Cancer Res. 2018 Jun 15;78(12):3135-3146. doi: 10.1158/0008-5472.CAN-17-3460. Epub 2018 May 3. PubMed PMID: 29724720; PubMed Central PMCID: PMC6004258.
Landino J, Norris SR, Li M, Ballister ER, Lampson MA, Ohi R. Two mechanisms coordinate the recruitment of the chromosomal passenger complex to the plane of cell division. Mol Biol Cell. 2017 Dec 1;28(25):3634-3646. doi: 10.1091/mbc.E17-06-0399. Epub 2017 Sep 27. PubMed PMID: 28954866; PubMed Central PMCID: PMC5706991.
Reinemann DN, Sturgill EG, Das DK, Degen MS, Vörös Z, Hwang W, Ohi R, Lang MJ. Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin Motors. Curr Biol. 2017 Sep 25;27(18):2810-2820.e6. doi: 10.1016/j.cub.2017.08.018. Epub 2017 Sep 14. PubMed PMID: 28918951; PubMed Central PMCID: PMC5909953.
Norris SR, Ohi R. Cell Division: Centrosomes Have Separation Anxiety. Curr Biol. 2017 Jun 19;27(12):R601-R603. doi: 10.1016/j.cub.2017.04.050. PubMed PMID: 28633031.
Chen GY, Kang YJ, Gayek AS, Youyen W, Tüzel E, Ohi R, Hancock WO. Eg5 Inhibitors Have Contrasting Effects on Microtubule Stability and Metaphase Spindle Integrity. ACS Chem Biol. 2017 Apr 21;12(4):1038-1046. doi: 10.1021/acschembio.6b01040. Epub 2017 Feb 22. PubMed PMID: 28165699; PubMed Central PMCID: PMC5515541.
Dumas ME, Sturgill EG, Ohi R. Resistance is not futile: Surviving Eg5 inhibition. Cell Cycle. 2016 Nov;15(21):2845-2847. doi: 10.1080/15384101.2016.1204864. Epub 2016 Jun 29. PubMed PMID: 27356105; PubMed Central PMCID: PMC5105924.
Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Mol Biol Cell. 2016 Jul 1;27(13):1990-9. doi: 10.1091/mbc.E15-12-0848. Epub 2016 May 4. PubMed PMID: 27146114; PubMed Central PMCID: PMC4927273.
Gayek AS, Ohi R. CDK-1 Inhibition in G2 Stabilizes Kinetochore-Microtubules in the following Mitosis. PLoS One. 2016;11(6):e0157491. doi: 10.1371/journal.pone.0157491. eCollection 2016. PubMed PMID: 27281342; PubMed Central PMCID: PMC4900577.
Sturgill EG, Norris SR, Guo Y, Ohi R. Kinesin-5 inhibitor resistance is driven by kinesin-12. J Cell Biol. 2016 Apr 25;213(2):213-27. doi: 10.1083/jcb.201507036. Epub 2016 Apr 18. PubMed PMID: 27091450; PubMed Central PMCID: PMC5084272.
Ohi R, Zanic M. Ahead of the Curve: New Insights into Microtubule Dynamics. F1000Res. 2016;5. doi: 10.12688/f1000research.7439.1. eCollection 2016. Review. PubMed PMID: 26998244; PubMed Central PMCID: PMC4786905.
Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Mol Biol Cell. 2016 Mar 9;27(9):1465-78. doi: 10.1091/mbc.E15-10-0725. [Epub ahead of print] PubMed PMID: 26960797; PubMed Central PMCID: PMC4850034.
Landino J, Ohi R. The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin. Curr Biol. 2016 Mar 7;26(5):698-706. doi: 10.1016/j.cub.2016.01.018. Epub 2016 Feb 18. PubMed PMID: 26898472; PubMed Central PMCID: PMC4783181.
Shin Y, Du Y, Collier SE, Ohi MD, Lang MJ, Ohi R. Biased Brownian motion as a mechanism to facilitate nanometer-scale exploration of the microtubule plus end by a kinesin-8. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3826-35. doi: 10.1073/pnas.1500272112. Epub 2015 Jul 6. PubMed PMID: 26150501; PubMed Central PMCID: PMC4517267.
Gayek AS, Ohi R. Fanning the flames of CIN. Cell Cycle. 2015;14(16):2560. doi: 10.1080/15384101.2015.1018053. PubMed PMID: 25714682; PubMed Central PMCID: PMC5242322.
Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry. 2014 Oct 21;53(41):6439-51. doi: 10.1021/bi5008639. Epub 2014 Oct 8. PubMed PMID: 25263959; PubMed Central PMCID: PMC4204884.
Sturgill EG, Das DK, Takizawa Y, Shin Y, Collier SE, Ohi MD, Hwang W, Lang MJ, Ohi R. Kinesin-12 Kif15 targets kinetochore fibers through an intrinsic two-step mechanism. Curr Biol. 2014 Oct 6;24(19):2307-13. doi: 10.1016/j.cub.2014.08.022. Epub 2014 Sep 25. PubMed PMID: 25264249; PubMed Central PMCID: PMC4207087.
Gayek AS, Ohi R. Kinetochore-microtubule stability governs the metaphase requirement for Eg5. Mol Biol Cell. 2014 Jul 1;25(13):2051-60. doi: 10.1091/mbc.E14-03-0785. Epub 2014 May 7. PubMed PMID: 24807901; PubMed Central PMCID: PMC4072578.
Sturgill EG, Ohi R. Microtubule-regulating kinesins. Curr Biol. 2013 Nov 4;23(21):R946-8. doi: 10.1016/j.cub.2013.08.012. PubMed PMID: 24200317.
Sturgill EG, Ohi R. Kinesin-12 differentially affects spindle assembly depending on its microtubule substrate. Curr Biol. 2013 Jul 22;23(14):1280-90. doi: 10.1016/j.cub.2013.05.043. Epub 2013 Jun 20. PubMed PMID: 23791727; PubMed Central PMCID: PMC3725213.
Walczak CE, Gayek S, Ohi R. Microtubule-depolymerizing kinesins. Annu Rev Cell Dev Biol. 2013;29:417-41. doi: 10.1146/annurev-cellbio-101512-122345. Epub 2013 Jul 17. Review. PubMed PMID: 23875646.
Su X, Ohi R, Pellman D. Move in for the kill: motile microtubule regulators. Trends Cell Biol. 2012 Nov;22(11):567-75. doi: 10.1016/j.tcb.2012.08.003. Epub 2012 Sep 6. Review. PubMed PMID: 22959403; PubMed Central PMCID: PMC3482944.
Ohi R, Wordeman L. Kinesins as Microtubule Disassembly Enzymes. In: Parent C, Coulombe P, editors. Encyclopedia of Biological Chemistry TBD (in press): Elsevier Press; 2012.
Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011 Nov 14;195(4):583-93. doi: 10.1083/jcb.201105074. Epub 2011 Nov 7. PubMed PMID: 22065639; PubMed Central PMCID: PMC3257533.
Archuleta TL, Du Y, English CA, Lory S, Lesser C, Ohi MD, Ohi R, Spiller BW. The Chlamydia effector chlamydial outer protein N (CopN) sequesters tubulin and prevents microtubule assembly. J Biol Chem. 2011 Sep 30;286(39):33992-8. doi: 10.1074/jbc.M111.258426. Epub 2011 Aug 13. PubMed PMID: 21841198; PubMed Central PMCID: PMC3190796.
Stumpff J, Du Y, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R. A tethering mechanism controls the processivity and kinetochore-microtubule plus-end enrichment of the kinesin-8 Kif18A. Mol Cell. 2011 Sep 2;43(5):764-75. doi: 10.1016/j.molcel.2011.07.022. PubMed PMID: 21884977; PubMed Central PMCID: PMC3172727.
Ohi R. Kip3-ing kinetochores clustered. Cell Cycle. 2010 Jul 1;9(13):2497. doi: 10.4161/cc.9.13.12274. PubMed PMID: 20647749; PubMed Central PMCID: PMC3233490.
Du Y, English CA, Ohi R. The kinesin-8 Kif18A dampens microtubule plus-end dynamics. Curr Biol. 2010 Feb 23;20(4):374-80. doi: 10.1016/j.cub.2009.12.049. Epub 2010 Feb 11. PubMed PMID: 20153196.
Needleman DJ, Groen A, Ohi R, Maresca T, Mirny L, Mitchison T. Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules. Mol Biol Cell. 2010 Jan 15;21(2):323-33. doi: 10.1091/mbc.e09-09-0816. Epub 2009 Nov 25. PubMed PMID: 19940016; PubMed Central PMCID: PMC2808228.
What would you like to do?