My Bibliography

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Saurabh S, Chong TN, Bayas C, Dahlberg PD, Cartwright HN, Moerner WE, Shapiro L. ATP-responsive biomolecular condensates tune bacterial kinase signaling. Sci Adv. 2022 Feb 18;8(7):eabm6570. doi: 10.1126/sciadv.abm6570. Epub 2022 Feb 16. PubMed PMID: 35171683; PubMed Central PMCID: PMC8849385.

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Wang J, Moerner WE, Shapiro L. A localized adaptor protein performs distinct functions at the Caulobacter cell poles. Proc Natl Acad Sci U S A. 2021 Mar 30;118(13). doi: 10.1073/pnas.2024705118. PubMed PMID: 33753507; PubMed Central PMCID: PMC8020655.

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Zhou X, Eckart MR, Shapiro L. A Bacterial Toxin Perturbs Intracellular Amino Acid Balance To Induce Persistence. mBio. 2021 Feb 23;12(1). doi: 10.1128/mBio.03020-20. PubMed PMID: 33622732; PubMed Central PMCID: PMC8545095.

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Lourenço RF, Saurabh S, Herrmann J, Wakatsuki S, Shapiro L. The Nucleoid-Associated Protein GapR Uses Conserved Structural Elements To Oligomerize and Bind DNA. mBio. 2020 Jun 9;11(3). doi: 10.1128/mBio.00448-20. PubMed PMID: 32518183; PubMed Central PMCID: PMC7373187.

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Dahlberg PD, Saurabh S, Sartor AM, Wang J, Mitchell PG, Chiu W, Shapiro L, Moerner WE. Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins in Caulobacter. Proc Natl Acad Sci U S A. 2020 Jun 23;117(25):13937-13944. doi: 10.1073/pnas.2001849117. Epub 2020 Jun 8. PubMed PMID: 32513734; PubMed Central PMCID: PMC7321984.

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Lasker K, von Diezmann L, Zhou X, Ahrens DG, Mann TH, Moerner WE, Shapiro L. Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus. Nat Microbiol. 2020 Mar;5(3):418-429. doi: 10.1038/s41564-019-0647-7. Epub 2020 Jan 20. PubMed PMID: 31959967; PubMed Central PMCID: PMC7549192.

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Herrmann J, Li PN, Jabbarpour F, Chan ACK, Rajkovic I, Matsui T, Shapiro L, Smit J, Weiss TM, Murphy MEP, Wakatsuki S. A bacterial surface layer protein exploits multistep crystallization for rapid self-assembly. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):388-394. doi: 10.1073/pnas.1909798116. Epub 2019 Dec 17. PubMed PMID: 31848245; PubMed Central PMCID: PMC6955313.

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Zhou X, Wang J, Herrmann J, Moerner WE, Shapiro L. Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation. Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15661-15670. doi: 10.1073/pnas.1906119116. Epub 2019 Jul 17. PubMed PMID: 31315982; PubMed Central PMCID: PMC6681729.

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Comerci CJ, Herrmann J, Yoon J, Jabbarpour F, Zhou X, Nomellini JF, Smit J, Shapiro L, Wakatsuki S, Moerner WE. Topologically-guided continuous protein crystallization controls bacterial surface layer self-assembly. Nat Commun. 2019 Jun 21;10(1):2731. doi: 10.1038/s41467-019-10650-x. PubMed PMID: 31227690; PubMed Central PMCID: PMC6588578.

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Dahlberg PD, Sartor AM, Wang J, Saurabh S, Shapiro L, Moerner WE. Identification of PAmKate as a Red Photoactivatable Fluorescent Protein for Cryogenic Super-Resolution Imaging. J Am Chem Soc. 2018 Oct 3;140(39):12310-12313. doi: 10.1021/jacs.8b05960. Epub 2018 Sep 20. PubMed PMID: 30222332; PubMed Central PMCID: PMC6174896.

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Mann TH, Shapiro L. Integration of cell cycle signals by multi-PAS domain kinases. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7166-E7173. doi: 10.1073/pnas.1808543115. Epub 2018 Jul 9. PubMed PMID: 29987042; PubMed Central PMCID: PMC6065003.

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Bayas CA, Wang J, Lee MK, Schrader JM, Shapiro L, Moerner WE. Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus. Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3712-E3721. doi: 10.1073/pnas.1721648115. Epub 2018 Apr 2. PubMed PMID: 29610352; PubMed Central PMCID: PMC5910860.

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Saurabh S, Perez AM, Comerci CJ, Shapiro L, Moerner WE. Super-Resolution Microscopy and Single-Protein Tracking in Live Bacteria Using a Genetically Encoded, Photostable Fluoromodule. Curr Protoc Cell Biol. 2017 Jun 19;75:4.32.1-4.32.22. doi: 10.1002/cpcb.21. PubMed PMID: 28627757; PubMed Central PMCID: PMC5768428.

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Herrmann J, Jabbarpour F, Bargar PG, Nomellini JF, Li PN, Lane TJ, Weiss TM, Smit J, Shapiro L, Wakatsuki S. Environmental Calcium Controls Alternate Physical States of the Caulobacter Surface Layer. Biophys J. 2017 May 9;112(9):1841-1851. doi: 10.1016/j.bpj.2017.04.003. PubMed PMID: 28494955; PubMed Central PMCID: PMC5425405.

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Perez AM, Mann TH, Lasker K, Ahrens DG, Eckart MR, Shapiro L. A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity. mBio. 2017 Feb 28;8(1). doi: 10.1128/mBio.02238-16. PubMed PMID: 28246363; PubMed Central PMCID: PMC5347347.

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Schrader JM, Li GW, Childers WS, Perez AM, Weissman JS, Shapiro L, McAdams HH. Dynamic translation regulation in Caulobacter cell cycle control. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6859-E6867. doi: 10.1073/pnas.1614795113. Epub 2016 Oct 17. PubMed PMID: 27791168; PubMed Central PMCID: PMC5098616.

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Ricci DP, Melfi MD, Lasker K, Dill DL, McAdams HH, Shapiro L. Cell cycle progression in Caulobacter requires a nucleoid-associated protein with high AT sequence recognition. Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5952-E5961. doi: 10.1073/pnas.1612579113. Epub 2016 Sep 19. PubMed PMID: 27647925; PubMed Central PMCID: PMC5056096.

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Saurabh S, Perez AM, Comerci CJ, Shapiro L, Moerner WE. Super-resolution Imaging of Live Bacteria Cells Using a Genetically Directed, Highly Photostable Fluoromodule. J Am Chem Soc. 2016 Aug 24;138(33):10398-401. doi: 10.1021/jacs.6b05943. Epub 2016 Aug 10. PubMed PMID: 27479076; PubMed Central PMCID: PMC4996739.

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Lasker K, Mann TH, Shapiro L. An intracellular compass spatially coordinates cell cycle modules in Caulobacter crescentus. Curr Opin Microbiol. 2016 Oct;33:131-139. doi: 10.1016/j.mib.2016.06.007. Epub 2016 Aug 10. Review. PubMed PMID: 27517351; PubMed Central PMCID: PMC5069156.

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Mann TH, Seth Childers W, Blair JA, Eckart MR, Shapiro L. A cell cycle kinase with tandem sensory PAS domains integrates cell fate cues. Nat Commun. 2016 Apr 27;7:11454. doi: 10.1038/ncomms11454. PubMed PMID: 27117914; PubMed Central PMCID: PMC4853435.

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Lasker K, Schrader JM, Men Y, Marshik T, Dill DL, McAdams HH, Shapiro L. CauloBrowser: A systems biology resource for Caulobacter crescentus. Nucleic Acids Res. 2016 Jan 4;44(D1):D640-5. doi: 10.1093/nar/gkv1050. Epub 2015 Oct 17. PubMed PMID: 26476443; PubMed Central PMCID: PMC4702786.

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Schrader JM, Shapiro L. Synchronization of Caulobacter crescentus for investigation of the bacterial cell cycle. J Vis Exp. 2015 Apr 8;(98). doi: 10.3791/52633. PubMed PMID: 25938623; PubMed Central PMCID: PMC4541484.

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Zhou B, Schrader JM, Kalogeraki VS, Abeliuk E, Dinh CB, Pham JQ, Cui ZZ, Dill DL, McAdams HH, Shapiro L. The global regulatory architecture of transcription during the Caulobacter cell cycle. PLoS Genet. 2015 Jan;11(1):e1004831. doi: 10.1371/journal.pgen.1004831. eCollection 2015 Jan. PubMed PMID: 25569173; PubMed Central PMCID: PMC4287350.

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Childers WS, Shapiro L. A pseudokinase couples signaling pathways to enable asymmetric cell division in a bacterium. Microb Cell. 2014 Dec 30;2(1):29-32. doi: 10.15698/mic2015.01.184. PubMed PMID: 28357261; PubMed Central PMCID: PMC5361649.

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Mera PE, Kalogeraki VS, Shapiro L. Replication initiator DnaA binds at the Caulobacter centromere and enables chromosome segregation. Proc Natl Acad Sci U S A. 2014 Nov 11;111(45):16100-5. doi: 10.1073/pnas.1418989111. Epub 2014 Oct 27. PubMed PMID: 25349407; PubMed Central PMCID: PMC4234595.

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Childers WS, Xu Q, Mann TH, Mathews II, Blair JA, Deacon AM, Shapiro L. Cell fate regulation governed by a repurposed bacterial histidine kinase. PLoS Biol. 2014 Oct;12(10):e1001979. doi: 10.1371/journal.pbio.1001979. eCollection 2014 Oct. PubMed PMID: 25349992; PubMed Central PMCID: PMC4211667.

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Williams B, Bhat N, Chien P, Shapiro L. ClpXP and ClpAP proteolytic activity on divisome substrates is differentially regulated following the Caulobacter asymmetric cell division. Mol Microbiol. 2014 Sep;93(5):853-66. doi: 10.1111/mmi.12698. Epub 2014 Aug 7. PubMed PMID: 24989075; PubMed Central PMCID: PMC4285227.

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Schrader JM, Zhou B, Li GW, Lasker K, Childers WS, Williams B, Long T, Crosson S, McAdams HH, Weissman JS, Shapiro L. The coding and noncoding architecture of the Caulobacter crescentus genome. PLoS Genet. 2014 Jul;10(7):e1004463. doi: 10.1371/journal.pgen.1004463. eCollection 2014 Jul. PubMed PMID: 25078267; PubMed Central PMCID: PMC4117421.

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Ptacin JL, Gahlmann A, Bowman GR, Perez AM, von Diezmann L, Eckart MR, Moerner WE, Shapiro L. Bacterial scaffold directs pole-specific centromere segregation. Proc Natl Acad Sci U S A. 2014 May 13;111(19):E2046-55. doi: 10.1073/pnas.1405188111. Epub 2014 Apr 28. PubMed PMID: 24778223; PubMed Central PMCID: PMC4024888.

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Gonzalez D, Kozdon JB, McAdams HH, Shapiro L, Collier J. The functions of DNA methylation by CcrM in Caulobacter crescentus: a global approach. Nucleic Acids Res. 2014 Apr;42(6):3720-35. doi: 10.1093/nar/gkt1352. Epub 2014 Jan 7. PubMed PMID: 24398711; PubMed Central PMCID: PMC3973325.

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Kozdon JB, Melfi MD, Luong K, Clark TA, Boitano M, Wang S, Zhou B, Gonzalez D, Collier J, Turner SW, Korlach J, Shapiro L, McAdams HH. Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle. Proc Natl Acad Sci U S A. 2013 Nov 26;110(48):E4658-67. doi: 10.1073/pnas.1319315110. Epub 2013 Nov 11. PubMed PMID: 24218615; PubMed Central PMCID: PMC3845142.

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Bowman GR, Perez AM, Ptacin JL, Ighodaro E, Folta-Stogniew E, Comolli LR, Shapiro L. Oligomerization and higher-order assembly contribute to sub-cellular localization of a bacterial scaffold. Mol Microbiol. 2013 Nov;90(4):776-95. doi: 10.1111/mmi.12398. Epub 2013 Oct 7. PubMed PMID: 24102805; PubMed Central PMCID: PMC3859194.

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Blair JA, Xu Q, Childers WS, Mathews II, Kern JW, Eckart M, Deacon AM, Shapiro L. Branched signal wiring of an essential bacterial cell-cycle phosphotransfer protein. Structure. 2013 Sep 3;21(9):1590-601. doi: 10.1016/j.str.2013.06.024. Epub 2013 Aug 8. PubMed PMID: 23932593; PubMed Central PMCID: PMC3787845.

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Gahlmann A, Ptacin JL, Grover G, Quirin S, von Diezmann L, Lee MK, Backlund MP, Shapiro L, Piestun R, Moerner WE. Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions. Nano Lett. 2013 Mar 13;13(3):987-93. doi: 10.1021/nl304071h. Epub 2013 Feb 25. PubMed PMID: 23414562; PubMed Central PMCID: PMC3599789.

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Hong SH, Toro E, Mortensen KI, de la Rosa MA, Doniach S, Shapiro L, Spakowitz AJ, McAdams HH. Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement. Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1674-9. doi: 10.1073/pnas.1220824110. Epub 2013 Jan 14. PubMed PMID: 23319648; PubMed Central PMCID: PMC3562846.

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Ptacin JL, Shapiro L. Chromosome architecture is a key element of bacterial cellular organization. Cell Microbiol. 2013 Jan;15(1):45-52. doi: 10.1111/cmi.12049. Epub 2012 Nov 20. Review. PubMed PMID: 23078580; PubMed Central PMCID: PMC3660146.

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Shapiro L. Life in a three-dimensional grid. J Biol Chem. 2012 Nov 2;287(45):38289-94. doi: 10.1074/jbc.X112.422337. Epub 2012 Sep 24. PubMed PMID: 23007401; PubMed Central PMCID: PMC3488097.

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Hocking J, Priyadarshini R, Takacs CN, Costa T, Dye NA, Shapiro L, Vollmer W, Jacobs-Wagner C. Osmolality-dependent relocation of penicillin-binding protein PBP2 to the division site in Caulobacter crescentus. J Bacteriol. 2012 Jun;194(12):3116-27. doi: 10.1128/JB.00260-12. Epub 2012 Apr 13. PubMed PMID: 22505677; PubMed Central PMCID: PMC3370875.

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Biteen JS, Goley ED, Shapiro L, Moerner WE. Three-dimensional super-resolution imaging of the midplane protein FtsZ in live Caulobacter crescentus cells using astigmatism. Chemphyschem. 2012 Mar;13(4):1007-12. doi: 10.1002/cphc.201100686. Epub 2012 Jan 20. PubMed PMID: 22262316; PubMed Central PMCID: PMC3712621.

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Xu Q, Christen B, Chiu HJ, Jaroszewski L, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Figurski DH, Shapiro L, Wilson IA. Structure of the pilus assembly protein TadZ from Eubacterium rectale: implications for polar localization. Mol Microbiol. 2012 Feb;83(4):712-27. doi: 10.1111/j.1365-2958.2011.07954.x. Epub 2012 Jan 18. PubMed PMID: 22211578; PubMed Central PMCID: PMC3272108.

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Biteen JS, Shapiro L, Moerner WE. Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging. Methods Mol Biol. 2011;783:139-58. doi: 10.1007/978-1-61779-282-3_8. PubMed PMID: 21909887; PubMed Central PMCID: PMC3702733.

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Lew MD, Lee SF, Ptacin JL, Lee MK, Twieg RJ, Shapiro L, Moerner WE. Three-dimensional superresolution colocalization of intracellular protein superstructures and the cell surface in live Caulobacter crescentus. Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):E1102-10. doi: 10.1073/pnas.1114444108. Epub 2011 Oct 26. PubMed PMID: 22031697; PubMed Central PMCID: PMC3219151.

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Umbarger MA, Toro E, Wright MA, Porreca GJ, Baù D, Hong SH, Fero MJ, Zhu LJ, Marti-Renom MA, McAdams HH, Shapiro L, Dekker J, Church GM. The three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation. Mol Cell. 2011 Oct 21;44(2):252-64. doi: 10.1016/j.molcel.2011.09.010. PubMed PMID: 22017872; PubMed Central PMCID: PMC3874842.

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Schwartz MA, Shapiro L. An SMC ATPase mutant disrupts chromosome segregation in Caulobacter. Mol Microbiol. 2011 Dec;82(6):1359-74. doi: 10.1111/j.1365-2958.2011.07836.x. Epub 2011 Sep 30. PubMed PMID: 21923769; PubMed Central PMCID: PMC3273039.

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Christen B, Abeliuk E, Collier JM, Kalogeraki VS, Passarelli B, Coller JA, Fero MJ, McAdams HH, Shapiro L. The essential genome of a bacterium. Mol Syst Biol. 2011 Aug 30;7:528. doi: 10.1038/msb.2011.58. PubMed PMID: 21878915; PubMed Central PMCID: PMC3202797.

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Dye NA, Pincus Z, Fisher IC, Shapiro L, Theriot JA. Mutations in the nucleotide binding pocket of MreB can alter cell curvature and polar morphology in Caulobacter. Mol Microbiol. 2011 Jul;81(2):368-94. doi: 10.1111/j.1365-2958.2011.07698.x. Epub 2011 May 26. PubMed PMID: 21564339; PubMed Central PMCID: PMC3137890.

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Goley ED, Yeh YC, Hong SH, Fero MJ, Abeliuk E, McAdams HH, Shapiro L. Assembly of the Caulobacter cell division machine. Mol Microbiol. 2011 Jun;80(6):1680-98. doi: 10.1111/j.1365-2958.2011.07677.x. Epub 2011 May 17. PubMed PMID: 21542856; PubMed Central PMCID: PMC3707389.

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Britos L, Abeliuk E, Taverner T, Lipton M, McAdams H, Shapiro L. Regulatory response to carbon starvation in Caulobacter crescentus. PLoS One. 2011 Apr 11;6(4):e18179. doi: 10.1371/journal.pone.0018179. PubMed PMID: 21494595; PubMed Central PMCID: PMC3073932.

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Lee SF, Thompson MA, Schwartz MA, Shapiro L, Moerner WE. Super-resolution imaging of the nucleoid-associated protein HU in Caulobacter crescentus. Biophys J. 2011 Apr 6;100(7):L31-3. doi: 10.1016/j.bpj.2011.02.022. PubMed PMID: 21463569; PubMed Central PMCID: PMC3072666.

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McAdams HH, Shapiro L. The architecture and conservation pattern of whole-cell control circuitry. J Mol Biol. 2011 May 27;409(1):28-35. doi: 10.1016/j.jmb.2011.02.041. Epub 2011 Mar 1. PubMed PMID: 21371478; PubMed Central PMCID: PMC3108490.