Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH, Sheu SS. Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes. Circ Res. 1996 Jul;79(1):86-93. doi: 10.1161/01.res.79.1.86. PubMed PMID: 8925573.
Sharma VK, Colecraft HM, Rubin LE, Sheu SS. Does mammalian heart contain only the M2 muscarinic receptor subtype?. Life Sci. 1997;60(13-14):1023-9. doi: 10.1016/s0024-3205(97)00043-x. PubMed PMID: 9121343.
Colecraft HM, Egamino JP, Sharma VK, Sheu SS. Signaling mechanisms underlying muscarinic receptor-mediated increase in contraction rate in cultured heart cells. J Biol Chem. 1998 Nov 27;273(48):32158-66. doi: 10.1074/jbc.273.48.32158. PubMed PMID: 9822693.
Colecraft HM, Patil PG, Yue DT. Differential occurrence of reluctant openings in G-protein-inhibited N- and P/Q-type calcium channels. J Gen Physiol. 2000 Feb;115(2):175-92. doi: 10.1085/jgp.115.2.175. PubMed PMID: 10653895; PubMed Central PMCID: PMC2217198.
Wei SK, Colecraft HM, DeMaria CD, Peterson BZ, Zhang R, Kohout TA, Rogers TB, Yue DT. Ca(2+) channel modulation by recombinant auxiliary beta subunits expressed in young adult heart cells. Circ Res. 2000 Feb 4;86(2):175-84. doi: 10.1161/01.res.86.2.175. PubMed PMID: 10666413.
Colecraft HM, Brody DL, Yue DT. G-protein inhibition of N- and P/Q-type calcium channels: distinctive elementary mechanisms and their functional impact. J Neurosci. 2001 Feb 15;21(4):1137-47. doi: 10.1523/JNEUROSCI.21-04-01137.2001. PubMed PMID: 11160384; PubMed Central PMCID: PMC6762231.
Colecraft HM, Alseikhan B, Takahashi SX, Chaudhuri D, Mittman S, Yegnasubramanian V, Alvania RS, Johns DC, Marbán E, Yue DT. Novel functional properties of Ca(2+) channel beta subunits revealed by their expression in adult rat heart cells. J Physiol. 2002 Jun 1;541(Pt 2):435-52. doi: 10.1113/jphysiol.2002.018515. PubMed PMID: 12042350; PubMed Central PMCID: PMC2290333.
Alseikhan BA, DeMaria CD, Colecraft HM, Yue DT. Engineered calmodulins reveal the unexpected eminence of Ca2+ channel inactivation in controlling heart excitation. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):17185-90. doi: 10.1073/pnas.262372999. Epub 2002 Dec 16. PubMed PMID: 12486220; PubMed Central PMCID: PMC139290.
Takahashi SX, Mittman S, Colecraft HM. Distinctive modulatory effects of five human auxiliary beta2 subunit splice variants on L-type calcium channel gating. Biophys J. 2003 May;84(5):3007-21. doi: 10.1016/S0006-3495(03)70027-7. PubMed PMID: 12719232; PubMed Central PMCID: PMC1302863.
Agler HL, Evans J, Colecraft HM, Yue DT. Custom distinctions in the interaction of G-protein beta subunits with N-type (CaV2.2) versus P/Q-type (CaV2.1) calcium channels. J Gen Physiol. 2003 Jun;121(6):495-510. doi: 10.1085/jgp.200208770. PubMed PMID: 12771191; PubMed Central PMCID: PMC2217353.
Takahashi SX, Miriyala J, Colecraft HM. Membrane-associated guanylate kinase-like properties of beta-subunits required for modulation of voltage-dependent Ca2+ channels. Proc Natl Acad Sci U S A. 2004 May 4;101(18):7193-8. doi: 10.1073/pnas.0306665101. Epub 2004 Apr 20. PubMed PMID: 15100405; PubMed Central PMCID: PMC406488.
Ganesan AN, O'Rourke B, Maack C, Colecraft H, Sidor A, Johns DC. Reverse engineering the L-type Ca2+ channel alpha1c subunit in adult cardiac myocytes using novel adenoviral vectors. Biochem Biophys Res Commun. 2005 Apr 8;329(2):749-54. doi: 10.1016/j.bbrc.2005.02.015. PubMed PMID: 15737650; PubMed Central PMCID: PMC2751644.
Agler HL, Evans J, Tay LH, Anderson MJ, Colecraft HM, Yue DT. G protein-gated inhibitory module of N-type (ca(v)2.2) ca2+ channels. Neuron. 2005 Jun 16;46(6):891-904. doi: 10.1016/j.neuron.2005.05.011. PubMed PMID: 15953418.
Dalton S, Takahashi SX, Miriyala J, Colecraft HM. A single CaVbeta can reconstitute both trafficking and macroscopic conductance of voltage-dependent calcium channels. J Physiol. 2005 Sep 15;567(Pt 3):757-69. doi: 10.1113/jphysiol.2005.093195. Epub 2005 Jul 14. PubMed PMID: 16020456; PubMed Central PMCID: PMC1474221.
Takahashi SX, Miriyala J, Tay LH, Yue DT, Colecraft HM. A CaVbeta SH3/guanylate kinase domain interaction regulates multiple properties of voltage-gated Ca2+ channels. J Gen Physiol. 2005 Oct;126(4):365-77. doi: 10.1085/jgp.200509354. PubMed PMID: 16186563; PubMed Central PMCID: PMC2266626.
Yang T, Suhail Y, Dalton S, Kernan T, Colecraft HM. Genetically encoded molecules for inducibly inactivating CaV channels. Nat Chem Biol. 2007 Dec;3(12):795-804. doi: 10.1038/nchembio.2007.42. Epub 2007 Oct 21. PubMed PMID: 17952065.
Bannister RA, Colecraft HM, Beam KG. Rem inhibits skeletal muscle EC coupling by reducing the number of functional L-type Ca2+ channels. Biophys J. 2008 Apr 1;94(7):2631-8. doi: 10.1529/biophysj.107.116467. Epub 2008 Jan 11. PubMed PMID: 18192376; PubMed Central PMCID: PMC2267126.
Miriyala J, Nguyen T, Yue DT, Colecraft HM. Role of CaVbeta subunits, and lack of functional reserve, in protein kinase A modulation of cardiac CaV1.2 channels. Circ Res. 2008 Apr 11;102(7):e54-64. doi: 10.1161/CIRCRESAHA.108.171736. Epub 2008 Mar 20. PubMed PMID: 18356540.
Pirone DM, Qi L, Colecraft H, Chen CS. Spatial patterning of gene expression using surface-immobilized recombinant adenovirus. Biomed Microdevices. 2008 Aug;10(4):561-6. doi: 10.1007/s10544-008-9166-7. PubMed PMID: 18246428.
Xu X, Colecraft HM. Primary culture of adult rat heart myocytes. J Vis Exp. 2009 Jun 16;(28). doi: 10.3791/1308. PubMed PMID: 19532115; PubMed Central PMCID: PMC2796664.
Xu X, Colecraft HM. Engineering proteins for custom inhibition of Ca(V) channels. Physiology (Bethesda). 2009 Aug;24:210-8. doi: 10.1152/physiol.00010.2009. Review. PubMed PMID: 19675352; PubMed Central PMCID: PMC2841218.
Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, Colecraft HM, Shaw RM. BIN1 localizes the L-type calcium channel to cardiac T-tubules. PLoS Biol. 2010 Feb 16;8(2):e1000312. doi: 10.1371/journal.pbio.1000312. PubMed PMID: 20169111; PubMed Central PMCID: PMC2821894.
Yang T, Xu X, Kernan T, Wu V, Colecraft HM. Rem, a member of the RGK GTPases, inhibits recombinant CaV1.2 channels using multiple mechanisms that require distinct conformations of the GTPase. J Physiol. 2010 May 15;588(Pt 10):1665-81. doi: 10.1113/jphysiol.2010.187203. Epub 2010 Mar 22. PubMed PMID: 20308247; PubMed Central PMCID: PMC2887986.
Xu X, Marx SO, Colecraft HM. Molecular mechanisms, and selective pharmacological rescue, of Rem-inhibited CaV1.2 channels in heart. Circ Res. 2010 Sep 3;107(5):620-30. doi: 10.1161/CIRCRESAHA.110.224717. Epub 2010 Jul 8. PubMed PMID: 20616312; PubMed Central PMCID: PMC2952418.
Fang K, Colecraft HM. Mechanism of auxiliary β-subunit-mediated membrane targeting of L-type (Ca(V)1.2) channels. J Physiol. 2011 Sep 15;589(Pt 18):4437-55. doi: 10.1113/jphysiol.2011.214247. Epub 2011 Jul 11. PubMed PMID: 21746784; PubMed Central PMCID: PMC3208217.
Hong TT, Smyth JW, Chu KY, Vogan JM, Fong TS, Jensen BC, Fang K, Halushka MK, Russell SD, Colecraft H, Hoopes CW, Ocorr K, Chi NC, Shaw RM. BIN1 is reduced and Cav1.2 trafficking is impaired in human failing cardiomyocytes. Heart Rhythm. 2012 May;9(5):812-20. doi: 10.1016/j.hrthm.2011.11.055. Epub 2011 Dec 2. PubMed PMID: 22138472; PubMed Central PMCID: PMC3306544.
Yang T, Puckerin A, Colecraft HM. Distinct RGK GTPases differentially use α1- and auxiliary β-binding-dependent mechanisms to inhibit CaV1.2/CaV2.2 channels. PLoS One. 2012;7(5):e37079. doi: 10.1371/journal.pone.0037079. Epub 2012 May 10. PubMed PMID: 22590648; PubMed Central PMCID: PMC3349659.
Yang T, He LL, Chen M, Fang K, Colecraft HM. Bio-inspired voltage-dependent calcium channel blockers. Nat Commun. 2013;4:2540. doi: 10.1038/ncomms3540. PubMed PMID: 24096474; PubMed Central PMCID: PMC4190111.
Shaw RM, Colecraft HM. L-type calcium channel targeting and local signalling in cardiac myocytes. Cardiovasc Res. 2013 May 1;98(2):177-86. doi: 10.1093/cvr/cvt021. Epub 2013 Feb 14. Review. PubMed PMID: 23417040; PubMed Central PMCID: PMC3633156.
Yang T, Colecraft HM. Regulation of voltage-dependent calcium channels by RGK proteins. Biochim Biophys Acta. 2013 Jul;1828(7):1644-54. doi: 10.1016/j.bbamem.2012.10.005. Epub 2012 Oct 10. Review. PubMed PMID: 23063948; PubMed Central PMCID: PMC4190112.
Subramanyam P, Chang DD, Fang K, Xie W, Marks AR, Colecraft HM. Manipulating L-type calcium channels in cardiomyocytes using split-intein protein transsplicing. Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15461-6. doi: 10.1073/pnas.1308161110. Epub 2013 Sep 3. PubMed PMID: 24003157; PubMed Central PMCID: PMC3780916.
Yang T, Liu Q, Kloss B, Bruni R, Kalathur RC, Guo Y, Kloppmann E, Rost B, Colecraft HM, Hendrickson WA. Structure and selectivity in bestrophin ion channels. Science. 2014 Oct 17;346(6207):355-9. doi: 10.1126/science.1259723. Epub 2014 Sep 25. PubMed PMID: 25324390; PubMed Central PMCID: PMC4341822.
Aromolaran AS, Subramanyam P, Chang DD, Kobertz WR, Colecraft HM. LQT1 mutations in KCNQ1 C-terminus assembly domain suppress IKs using different mechanisms. Cardiovasc Res. 2014 Dec 1;104(3):501-11. doi: 10.1093/cvr/cvu231. Epub 2014 Oct 24. PubMed PMID: 25344363; PubMed Central PMCID: PMC4296111.
Yang T, Hendrickson WA, Colecraft HM. Preassociated apocalmodulin mediates Ca2+-dependent sensitization of activation and inactivation of TMEM16A/16B Ca2+-gated Cl- channels. Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):18213-8. doi: 10.1073/pnas.1420984111. Epub 2014 Dec 8. PubMed PMID: 25489088; PubMed Central PMCID: PMC4280629.
Subramanyam P, Colecraft HM. Ion channel engineering: perspectives and strategies. J Mol Biol. 2015 Jan 16;427(1):190-204. doi: 10.1016/j.jmb.2014.09.001. Epub 2014 Sep 7. Review. PubMed PMID: 25205552; PubMed Central PMCID: PMC4289156.
Chang DD, Colecraft HM. Rad and Rem are non-canonical G-proteins with respect to the regulatory role of guanine nucleotide binding in Ca(V)1.2 channel regulation. J Physiol. 2015 Dec 1;593(23):5075-90. doi: 10.1113/JP270889. PubMed PMID: 26426338; PubMed Central PMCID: PMC4666999.
Yang T, Colecraft HM. Calmodulin regulation of TMEM16A and 16B Ca(2+)-activated chloride channels. Channels (Austin). 2016;10(1):38-44. doi: 10.1080/19336950.2015.1058455. Epub 2015 Jun 17. Review. PubMed PMID: 26083059; PubMed Central PMCID: PMC4802756.
Joseph LC, Barca E, Subramanyam P, Komrowski M, Pajvani U, Colecraft HM, Hirano M, Morrow JP. Inhibition of NAPDH Oxidase 2 (NOX2) Prevents Oxidative Stress and Mitochondrial Abnormalities Caused by Saturated Fat in Cardiomyocytes. PLoS One. 2016;11(1):e0145750. doi: 10.1371/journal.pone.0145750. eCollection 2016. PubMed PMID: 26756466; PubMed Central PMCID: PMC4710525.
Puckerin A, Aromolaran KA, Chang DD, Zukin RS, Colecraft HM, Boutjdir M, Aromolaran AS. hERG 1a LQT2 C-terminus truncation mutants display hERG 1b-dependent dominant negative mechanisms. Heart Rhythm. 2016 May;13(5):1121-1130. doi: 10.1016/j.hrthm.2016.01.012. Epub 2016 Jan 13. PubMed PMID: 26775140.
Aromolaran AS, Colecraft HM, Boutjdir M. High-fat diet-dependent modulation of the delayed rectifier K(+) current in adult guinea pig atrial myocytes. Biochem Biophys Res Commun. 2016 Jun 3;474(3):554-559. doi: 10.1016/j.bbrc.2016.04.113. Epub 2016 Apr 26. PubMed PMID: 27130822.
Geng Y, Mosyak L, Kurinov I, Zuo H, Sturchler E, Cheng TC, Subramanyam P, Brown AP, Brennan SC, Mun HC, Bush M, Chen Y, Nguyen TX, Cao B, Chang DD, Quick M, Conigrave AD, Colecraft HM, McDonald P, Fan QR. Structural mechanism of ligand activation in human calcium-sensing receptor. Elife. 2016 Jul 19;5. doi: 10.7554/eLife.13662. PubMed PMID: 27434672; PubMed Central PMCID: PMC4977154.
Puckerin AA, Chang DD, Subramanyam P, Colecraft HM. Similar molecular determinants on Rem mediate two distinct modes of inhibition of Ca(V)1.2 channels. Channels (Austin). 2016 Sep 2;10(5):379-394. doi: 10.1080/19336950.2016.1180489. Epub 2016 Apr 26. PubMed PMID: 27115600; PubMed Central PMCID: PMC4988437.
Ortuño MJ, Robinson ST, Subramanyam P, Paone R, Huang YY, Guo XE, Colecraft HM, Mann JJ, Ducy P. Serotonin-reuptake inhibitors act centrally to cause bone loss in mice by counteracting a local anti-resorptive effect. Nat Med. 2016 Oct;22(10):1170-1179. doi: 10.1038/nm.4166. Epub 2016 Sep 5. PubMed PMID: 27595322; PubMed Central PMCID: PMC5053870.
Hu Z, Wang JW, Yu D, Soon JL, de Kleijn DP, Foo R, Liao P, Colecraft HM, Soong TW. Aberrant Splicing Promotes Proteasomal Degradation of L-type Ca(V)1.2 Calcium Channels by Competitive Binding for Ca(V)β Subunits in Cardiac Hypertrophy. Sci Rep. 2016 Oct 12;6:35247. doi: 10.1038/srep35247. PubMed PMID: 27731386; PubMed Central PMCID: PMC5059693.
Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca(2+) channels. Elife. 2017 Jan 6;6. doi: 10.7554/eLife.21989. PubMed PMID: 28059704; PubMed Central PMCID: PMC5279948.
Grandi E, Sanguinetti MC, Bartos DC, Bers DM, Chen-Izu Y, Chiamvimonvat N, Colecraft HM, Delisle BP, Heijman J, Navedo MF, Noskov S, Proenza C, Vandenberg JI, Yarov-Yarovoy V. Potassium channels in the heart: structure, function and regulation. J Physiol. 2017 Apr 1;595(7):2209-2228. doi: 10.1113/JP272864. Epub 2016 Nov 13. Review. PubMed PMID: 27861921; PubMed Central PMCID: PMC5374109.
Katchman A, Yang L, Zakharov SI, Kushner J, Abrams J, Chen BX, Liu G, Pitt GS, Colecraft HM, Marx SO. Proteolytic cleavage and PKA phosphorylation of α(1C) subunit are not required for adrenergic regulation of Ca(V)1.2 in the heart. Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):9194-9199. doi: 10.1073/pnas.1706054114. Epub 2017 Aug 7. PubMed PMID: 28784807; PubMed Central PMCID: PMC5576811.
Joseph LC, Kokkinaki D, Valenti MC, Kim GJ, Barca E, Tomar D, Hoffman NE, Subramanyam P, Colecraft HM, Hirano M, Ratner AJ, Madesh M, Drosatos K, Morrow JP. Inhibition of NADPH oxidase 2 (NOX2) prevents sepsis-induced cardiomyopathy by improving calcium handling and mitochondrial function. JCI Insight. 2017 Sep 7;2(17). doi: 10.1172/jci.insight.94248. eCollection 2017 Sep 7. PubMed PMID: 28878116; PubMed Central PMCID: PMC5621873.
Kanner SA, Morgenstern T, Colecraft HM. Sculpting ion channel functional expression with engineered ubiquitin ligases. Elife. 2017 Dec 19;6. doi: 10.7554/eLife.29744. PubMed PMID: 29256394; PubMed Central PMCID: PMC5764571.
Shuja Z, Colecraft HM. Regulation of microdomain voltage-gated L-type calcium channels in cardiac health and disease. Curr Opin Physiol. 2018 Apr;2:13-18. doi: 10.1016/j.cophys.2017.12.005. Epub 2017 Dec 23. PubMed PMID: 29963649; PubMed Central PMCID: PMC6022851.
What would you like to do?