Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Astashkin AV, Gyawali YP, Jiang T, Zhang H, Feng C. Analyzing the FMN-heme interdomain docking interactions in neuronal and inducible NOS isoforms by pulsed EPR experiments and conformational distribution modeling. J Biol Inorg Chem. 2024 Sep;29(6):611-623. doi: 10.1007/s00775-024-02068-8. Epub 2024 Aug 13. PubMed PMID: 39136772; PubMed Central PMCID: PMC11390318.
Jiang T, Wan G, Zhang H, Gyawali YP, Underbakke ES, Feng C. Mapping the Intersubunit Interdomain FMN-Heme Interactions in Neuronal Nitric Oxide Synthase by Targeted Quantitative Cross-Linking Mass Spectrometry. Biochemistry. 2024 Jun 4;63(11):1395-1411. doi: 10.1021/acs.biochem.4c00157. Epub 2024 May 15. PubMed PMID: 38747545.
Wardhani K, Yazzie S, McVeigh C, Edeh O, Grimes M, Jacquez Q, Dixson C, Barr E, Liu R, Bolt AM, Feng C, Zychowski KE. Systemic immunological responses are dependent on sex and ovarian hormone presence following acute inhaled woodsmoke exposure. Part Fibre Toxicol. 2024 May 27;21(1):27. doi: 10.1186/s12989-024-00587-5. PubMed PMID: 38797836; PubMed Central PMCID: PMC11129474.
Singh S, Gyawali YP, Jiang T, Bukowski GS, Zheng H, Zhang H, Owopetu R, Thielges MC, Feng C. Probing calmodulin-NO synthase interactions via site-specific infrared spectroscopy: an introductory investigation. J Biol Inorg Chem. 2024 Mar;29(2):243-250. doi: 10.1007/s00775-024-02046-0. Epub 2024 Apr 5. PubMed PMID: 38580821; PubMed Central PMCID: PMC11181464.
Gyawali YP, Jiang T, Yang J, Zheng H, Liu R, Zhang H, Feng C. Differential superoxide production in phosphorylated neuronal nitric oxide synthase mu and alpha variants. J Inorg Biochem. 2024 Feb;251:112454. doi: 10.1016/j.jinorgbio.2023.112454. Epub 2023 Dec 11. PubMed PMID: 38100901; PubMed Central PMCID: PMC10843652.
Jiang T, Wan G, Zhang H, Gyawali YP, Underbakke ES, Feng C. Probing Protein Dynamics in Neuronal Nitric Oxide Synthase by Quantitative Cross-Linking Mass Spectrometry. Biochemistry. 2023 Aug 1;62(15):2232-2237. doi: 10.1021/acs.biochem.3c00245. Epub 2023 Jul 17. PubMed PMID: 37459398; PubMed Central PMCID: PMC10529231.
Tumbic GW, Li J, Jiang T, Hossan MY, Feng C, Thielges MC. Interdomain Interactions Modulate the Active Site Dynamics of Human Inducible Nitric Oxide Synthase. J Phys Chem B. 2022 Sep 15;126(36):6811-6819. doi: 10.1021/acs.jpcb.2c04091. Epub 2022 Sep 3. PubMed PMID: 36056879; PubMed Central PMCID: PMC10110350.
Lu JY, Chen QY, Meng SC, Feng CJ. A dye-andrographolide assembly as a turn-on sensor for detection of phthalate in both cells and fish. Anal Chim Acta. 2022 Feb 22;1195:339460. doi: 10.1016/j.aca.2022.339460. Epub 2022 Jan 10. PubMed PMID: 35090651.
Zheng H, Weaver JM, Feng C. Heat shock protein 90α increases superoxide generation from neuronal nitric oxide synthases. J Inorg Biochem. 2021 Jan;214:111298. doi: 10.1016/j.jinorgbio.2020.111298. Epub 2020 Nov 4. PubMed PMID: 33181440; PubMed Central PMCID: PMC7719093.
Zheng H, Li J, Feng C. An isoform-specific pivot modulates the electron transfer between the flavin mononucleotide and heme centers in inducible nitric oxide synthase. J Biol Inorg Chem. 2020 Dec;25(8):1097-1105. doi: 10.1007/s00775-020-01824-w. Epub 2020 Oct 14. PubMed PMID: 33057871; PubMed Central PMCID: PMC7669679.
Zheng H, Li J, Feng C. Heat shock protein 90 enhances the electron transfer between the FMN and heme cofactors in neuronal nitric oxide synthase. FEBS Lett. 2020 Sep;594(17):2904-2913. doi: 10.1002/1873-3468.13870. Epub 2020 Jul 4. PubMed PMID: 32573772; PubMed Central PMCID: PMC7722166.
Yang R, Fang XL, Zhen Q, Chen QY, Feng C. Mitochondrial targeting nano-curcumin for attenuation on PKM2 and FASN. Colloids Surf B Biointerfaces. 2019 Oct 1;182:110405. doi: 10.1016/j.colsurfb.2019.110405. Epub 2019 Jul 29. PubMed PMID: 31377611.
Astashkin AV, Li J, Zheng H, Feng C. Positional Distributions of the Tethered Modules in Nitric Oxide Synthase: Monte Carlo Calculations and Pulsed EPR Measurements. J Phys Chem A. 2019 Aug 15;123(32):7075-7086. doi: 10.1021/acs.jpca.9b05388. Epub 2019 Aug 2. PubMed PMID: 31310526; PubMed Central PMCID: PMC7534783.
Li J, Zheng H, Feng C. Effect of Macromolecular Crowding on the FMN-Heme Intraprotein Electron Transfer in Inducible NO Synthase. Biochemistry. 2019 Jul 16;58(28):3087-3096. doi: 10.1021/acs.biochem.9b00193. Epub 2019 Jun 28. PubMed PMID: 31251033; PubMed Central PMCID: PMC7534385.
Zheng H, He J, Li J, Yang J, Kirk ML, Roman LJ, Feng C. Generation and characterization of functional phosphoserine-incorporated neuronal nitric oxide synthase holoenzyme. J Biol Inorg Chem. 2019 Feb;24(1):1-9. doi: 10.1007/s00775-018-1621-1. Epub 2018 Oct 12. PubMed PMID: 30315355; PubMed Central PMCID: PMC6384131.
Yang J, Kersi DK, Richers CP, Giles LJ, Dangi R, Stein BW, Feng C, Tichnell CR, Shultz DA, Kirk ML. Ground State Nuclear Magnetic Resonance Chemical Shifts Predict Charge-Separated Excited State Lifetimes. Inorg Chem. 2018 Nov 5;57(21):13470-13476. doi: 10.1021/acs.inorgchem.8b02087. Epub 2018 Oct 9. PubMed PMID: 30299098.
Astashkin AV, Li J, Zheng H, Miao Y, Feng C. A docked state conformational dynamics model to explain the ionic strength dependence of FMN - heme electron transfer in nitric oxide synthase. J Inorg Biochem. 2018 Jul;184:146-155. doi: 10.1016/j.jinorgbio.2018.03.012. Epub 2018 Mar 26. PubMed PMID: 29751215; PubMed Central PMCID: PMC5983377.
Li J, Zheng H, Wang W, Miao Y, Sheng Y, Feng C. Role of an isoform-specific residue at the calmodulin-heme (NO synthase) interface in the FMN - heme electron transfer. FEBS Lett. 2018 Jul;592(14):2425-2431. doi: 10.1002/1873-3468.13158. Epub 2018 Jun 29. PubMed PMID: 29904908; PubMed Central PMCID: PMC6105432.
Li J, Zheng H, Feng C. Deciphering mechanism of conformationally controlled electron transfer in nitric oxide synthases. Front Biosci (Landmark Ed). 2018 Jun 1;23(10):1803-1821. doi: 10.2741/4674. Review. PubMed PMID: 29772530; PubMed Central PMCID: PMC11167721.
Liu L, Xu J, Yang J, Feng C, Miao Y. Metastatic melanoma imaging using a novel Tc-99m-labeled lactam-cyclized alpha-MSH peptide. Bioorg Med Chem Lett. 2017 Nov 15;27(22):4952-4955. doi: 10.1016/j.bmcl.2017.10.022. Epub 2017 Oct 12. PubMed PMID: 29054361; PubMed Central PMCID: PMC5667642.
Xu J, Feng C, Miao Y. Evaluation of novel (111)In-labeled gonadotropin-releasing hormone peptides for human prostate cancer imaging. Bioorg Med Chem Lett. 2017 Oct 15;27(20):4647-4651. doi: 10.1016/j.bmcl.2017.09.004. Epub 2017 Sep 7. PubMed PMID: 28917649; PubMed Central PMCID: PMC5726392.
McQuarters AB, Speelman AL, Chen L, Elmore BO, Fan W, Feng C, Lehnert N. Exploring second coordination sphere effects in nitric oxide synthase. J Biol Inorg Chem. 2016 Dec;21(8):997-1008. doi: 10.1007/s00775-016-1396-1. Epub 2016 Sep 29. PubMed PMID: 27686338; PubMed Central PMCID: PMC5541774.
Chen L, Zheng H, Li W, Li W, Miao Y, Feng C. Role of a Conserved Tyrosine Residue in the FMN-Heme Interdomain Electron Transfer in Inducible Nitric Oxide Synthase. J Phys Chem A. 2016 Oct 6;120(39):7610-7616. doi: 10.1021/acs.jpca.6b08207. Epub 2016 Sep 27. PubMed PMID: 27633182; PubMed Central PMCID: PMC5526657.
Huestis J, Zhou X, Chen L, Feng C, Hudson LG, Liu KJ. Kinetics and thermodynamics of zinc(II) and arsenic(III) binding to XPA and PARP-1 zinc finger peptides. J Inorg Biochem. 2016 Oct;163:45-52. doi: 10.1016/j.jinorgbio.2016.08.003. Epub 2016 Aug 2. PubMed PMID: 27521476; PubMed Central PMCID: PMC5096954.
Liu L, Xu J, Yang J, Feng C, Miao Y. Imaging human melanoma using a novel Tc-99m-labeled lactam bridge-cyclized alpha-MSH peptide. Bioorg Med Chem Lett. 2016 Oct 1;26(19):4724-4728. doi: 10.1016/j.bmcl.2016.08.042. Epub 2016 Aug 17. PubMed PMID: 27568083; PubMed Central PMCID: PMC5018458.
Xu XL, Shao J, Chen QY, Li CH, Kong MY, Fang F, Ji L, Boison D, Huang T, Gao J, Feng CJ. A Mn(II) complex of boradiazaindacene (BODIPY) loaded graphene oxide as both LED light and H2O2 enhanced anticancer agent. J Inorg Biochem. 2016 Jun;159:1-6. doi: 10.1016/j.jinorgbio.2016.02.007. Epub 2016 Feb 13. PubMed PMID: 26901626.
Sheng Y, Zhong L, Guo D, Lau G, Feng C. Insight into structural rearrangements and interdomain interactions related to electron transfer between flavin mononucleotide and heme in nitric oxide synthase: A molecular dynamics study. J Inorg Biochem. 2015 Dec;153:186-196. doi: 10.1016/j.jinorgbio.2015.08.006. Epub 2015 Aug 7. PubMed PMID: 26277414; PubMed Central PMCID: PMC4690761.
Astashkin AV, Feng C. Solving Kinetic Equations for the Laser Flash Photolysis Experiment on Nitric Oxide Synthases: Effect of Conformational Dynamics on the Interdomain Electron Transfer. J Phys Chem A. 2015 Nov 12;119(45):11066-75. doi: 10.1021/acs.jpca.5b08414. Epub 2015 Oct 30. PubMed PMID: 26477677; PubMed Central PMCID: PMC5518706.
Astashkin AV, Chen L, Elmore BO, Kunwar D, Miao Y, Li H, Poulos TL, Roman LJ, Feng C. Probing the Hydrogen Bonding of the Ferrous-NO Heme Center of nNOS by Pulsed Electron Paramagnetic Resonance. J Phys Chem A. 2015 Jun 25;119(25):6641-9. doi: 10.1021/acs.jpca.5b01804. Epub 2015 Jun 12. PubMed PMID: 26035438; PubMed Central PMCID: PMC4533915.
Astashkin AV, Chen L, Zhou X, Li H, Poulos TL, Liu KJ, Guillemette JG, Feng C. Pulsed electron paramagnetic resonance study of domain docking in neuronal nitric oxide synthase: the calmodulin and output state perspective. J Phys Chem A. 2014 Aug 28;118(34):6864-72. doi: 10.1021/jp503547w. Epub 2014 Jul 31. PubMed PMID: 25046446; PubMed Central PMCID: PMC4148148.
Yang J, Kersi DK, Giles LJ, Stein BW, Feng C, Tichnell CR, Shultz DA, Kirk ML. Ligand control of donor-acceptor excited-state lifetimes. Inorg Chem. 2014 May 19;53(10):4791-3. doi: 10.1021/ic500217y. Epub 2014 Apr 28. PubMed PMID: 24773363.
Lu J, Hathaway HJ, Royce ME, Prossnitz ER, Miao Y. Introduction of D-phenylalanine enhanced the receptor binding affinities of gonadotropin-releasing hormone peptides. Bioorg Med Chem Lett. 2014 Feb 1;24(3):725-30. doi: 10.1016/j.bmcl.2013.12.120. Epub 2014 Jan 6. PubMed PMID: 24418777; PubMed Central PMCID: PMC3924789.
Yang J, Flook AM, Feng C, Miao Y. Linker modification reduced the renal uptake of technetium-99m-labeled Arg-Ala-Asp-conjugated alpha-melanocyte stimulating hormone peptide. Bioorg Med Chem Lett. 2014 Jan 1;24(1):195-8. doi: 10.1016/j.bmcl.2013.11.042. Epub 2013 Nov 23. PubMed PMID: 24316121; PubMed Central PMCID: PMC3889140.
Feng C, Chen L, Li W, Elmore BO, Fan W, Sun X. Dissecting regulation mechanism of the FMN to heme interdomain electron transfer in nitric oxide synthases. J Inorg Biochem. 2014 Jan;130:130-40. doi: 10.1016/j.jinorgbio.2013.09.005. Epub 2013 Sep 13. Review. PubMed PMID: 24084585; PubMed Central PMCID: PMC3844001.
Panda SP, Li W, Venkatakrishnan P, Chen L, Astashkin AV, Masters BS, Feng C, Roman LJ. Differential calmodulin-modulatory and electron transfer properties of neuronal nitric oxide synthase mu compared to the alpha variant. FEBS Lett. 2013 Dec 11;587(24):3973-8. doi: 10.1016/j.febslet.2013.10.032. Epub 2013 Nov 6. PubMed PMID: 24211446; PubMed Central PMCID: PMC3897158.
Guo H, Hathaway H, Royce ME, Prossnitz ER, Miao Y. Influences of hydrocarbon linkers on the receptor binding affinities of gonadotropin-releasing hormone peptides. Bioorg Med Chem Lett. 2013 Oct 15;23(20):5484-7. doi: 10.1016/j.bmcl.2013.08.075. Epub 2013 Aug 23. PubMed PMID: 24018188; PubMed Central PMCID: PMC3778074.
Li W, Chen L, Lu C, Elmore BO, Astashkin AV, Rousseau DL, Yeh SR, Feng C. Regulatory role of Glu546 in flavin mononucleotide-heme electron transfer in human inducible nitric oxide synthase. Inorg Chem. 2013 May 6;52(9):4795-801. doi: 10.1021/ic3020892. Epub 2013 Apr 9. PubMed PMID: 23570607; PubMed Central PMCID: PMC3677850.
Astashkin AV, Elmore BO, Chen L, Fan W, Guillemette JG, Feng C. Pulsed ENDOR determination of the arginine location in the ferrous-NO form of neuronal NOS. J Phys Chem A. 2012 Jun 28;116(25):6731-9. doi: 10.1021/jp302319c. Epub 2012 Jun 15. PubMed PMID: 22667467; PubMed Central PMCID: PMC3386476.
Li W, Fan W, Chen L, Elmore BO, Piazza M, Guillemette JG, Feng C. Role of an isoform-specific serine residue in FMN-heme electron transfer in inducible nitric oxide synthase. J Biol Inorg Chem. 2012 Jun;17(5):675-85. doi: 10.1007/s00775-012-0887-y. Epub 2012 Mar 10. PubMed PMID: 22407542; PubMed Central PMCID: PMC3526944.
Feng C. Mechanism of Nitric Oxide Synthase Regulation: Electron Transfer and Interdomain Interactions. Coord Chem Rev. 2012 Feb 1;256(3-4):393-411. doi: 10.1016/j.ccr.2011.10.011. Epub 2011 Oct 17. PubMed PMID: 22523434; PubMed Central PMCID: PMC3328867.
Li W, Chen L, Fan W, Feng C. Comparing the temperature dependence of FMN to heme electron transfer in full length and truncated inducible nitric oxide synthase proteins. FEBS Lett. 2012 Jan 20;586(2):159-62. doi: 10.1016/j.febslet.2011.12.009. Epub 2011 Dec 17. PubMed PMID: 22198200; PubMed Central PMCID: PMC3259286.
Feng C, Taiakina V, Ghosh DK, Guillemette JG, Tollin G. Intraprotein electron transfer between the FMN and heme domains in endothelial nitric oxide synthase holoenzyme. Biochim Biophys Acta. 2011 Dec;1814(12):1997-2002. doi: 10.1016/j.bbapap.2011.08.004. Epub 2011 Aug 16. PubMed PMID: 21864726; PubMed Central PMCID: PMC3223294.
Astashkin AV, Fan W, Elmore BO, Guillemette JG, Feng C. Pulsed ENDOR determination of relative orientation of g-frame and molecular frame of imidazole-coordinated heme center of iNOS. J Phys Chem A. 2011 Sep 22;115(37):10345-52. doi: 10.1021/jp204969d. Epub 2011 Aug 26. PubMed PMID: 21834532; PubMed Central PMCID: PMC3174316.
Li W, Fan W, Elmore BO, Feng C. Effect of solution viscosity on intraprotein electron transfer between the FMN and heme domains in inducible nitric oxide synthase. FEBS Lett. 2011 Aug 19;585(16):2622-6. doi: 10.1016/j.febslet.2011.07.022. Epub 2011 Jul 26. PubMed PMID: 21803041; PubMed Central PMCID: PMC3163105.
Sempombe J, Galinato MG, Elmore BO, Fan W, Guillemette JG, Lehnert N, Kirk ML, Feng C. Mutation in the flavin mononucleotide domain modulates magnetic circular dichroism spectra of the iNOS ferric cyano complex in a substrate-specific manner. Inorg Chem. 2011 Aug 1;50(15):6859-61. doi: 10.1021/ic200952c. Epub 2011 Jun 30. PubMed PMID: 21718007; PubMed Central PMCID: PMC3143224.
Feng C, Fan W, Ghosh DK, Tollin G. Role of an isoform-specific substrate access channel residue in CO ligand accessibilities of neuronal and inducible nitric oxide synthase isoforms. Biochim Biophys Acta. 2011 Mar;1814(3):405-8. doi: 10.1016/j.bbapap.2010.11.007. Epub 2010 Dec 10. PubMed PMID: 21146639; PubMed Central PMCID: PMC3039037.
Feng C, Fan W, Dupont A, Guy Guillemette J, Ghosh DK, Tollin G. Electron transfer in a human inducible nitric oxide synthase oxygenase/FMN construct co-expressed with the N-terminal globular domain of calmodulin. FEBS Lett. 2010 Oct 22;584(20):4335-8. doi: 10.1016/j.febslet.2010.09.028. Epub 2010 Sep 23. PubMed PMID: 20868689; PubMed Central PMCID: PMC2956772.
Astashkin AV, Elmore BO, Fan W, Guillemette JG, Feng C. Pulsed EPR determination of the distance between heme iron and FMN centers in a human inducible nitric oxide synthase. J Am Chem Soc. 2010 Sep 1;132(34):12059-67. doi: 10.1021/ja104461p. PubMed PMID: 20695464; PubMed Central PMCID: PMC2931817.
Feng C, Tollin G. Regulation of interdomain electron transfer in the NOS output state for NO production. Dalton Trans. 2009 Sep 14;(34):6692-700. doi: 10.1039/b902884f. Epub 2009 Jun 17. PubMed PMID: 19690675; PubMed Central PMCID: PMC2997718.
Sempombe J, Elmore BO, Sun X, Dupont A, Ghosh DK, Guillemette JG, Kirk ML, Feng C. Mutations in the FMN domain modulate MCD spectra of the heme site in the oxygenase domain of inducible nitric oxide synthase. J Am Chem Soc. 2009 May 27;131(20):6940-1. doi: 10.1021/ja902141v. PubMed PMID: 19405537; PubMed Central PMCID: PMC2754207.
What would you like to do?