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Fisher IJ, Jenkins ML, Tall GG, Burke JE, Smrcka AV. Activation of Phospholipase C β by Gβγ and Gαq Involves C-Terminal Rearrangement to Release Autoinhibition. Structure. 2020 Jul 7;28(7):810-819.e5. doi: 10.1016/j.str.2020.04.012. Epub 2020 May 12. PubMed PMID: 32402248.
Atchison DK, O'Connor CL, Menon R, Otto EA, Ganesh SK, Wiggins RC, Smrcka AV, Bitzer M. Hypertension induces glomerulosclerosis in phospholipase C-ε1 deficiency. Am J Physiol Renal Physiol. 2020 May 1;318(5):F1177-F1187. doi: 10.1152/ajprenal.00541.2019. Epub 2020 Mar 30. PubMed PMID: 32223311; PubMed Central PMCID: PMC7294338.
Strazza M, Adam K, Smrcka AV, Lerrer S, Mor A. PLCε1 suppresses tumor growth by regulating murine T cell mobilization. Clin Exp Immunol. 2020 Apr;200(1):53-60. doi: 10.1111/cei.13409. Epub 2020 Jan 9. PubMed PMID: 31867717; PubMed Central PMCID: PMC7066383.
Cash JN, Chandan NR, Hsu AY, Sharma PV, Deng Q, Smrcka AV, Tesmer JJG. Discovery of Small Molecules That Target the Phosphatidylinositol (3,4,5) Trisphosphate (PIP3)-Dependent Rac Exchanger 1 (P-Rex1) PIP3-Binding Site and Inhibit P-Rex1-Dependent Functions in Neutrophils. Mol Pharmacol. 2020 Mar;97(3):226-236. doi: 10.1124/mol.119.117556. Epub 2020 Jan 3. PubMed PMID: 31900312; PubMed Central PMCID: PMC6994817.
Smrcka AV, Fisher I. G-protein βγ subunits as multi-functional scaffolds and transducers in G-protein-coupled receptor signaling. Cell Mol Life Sci. 2019 Nov;76(22):4447-4459. doi: 10.1007/s00018-019-03275-2. Epub 2019 Aug 21. Review. PubMed PMID: 31435698; PubMed Central PMCID: PMC6842434.
Nash CA, Wei W, Irannejad R, Smrcka AV. Golgi localized β1-adrenergic receptors stimulate Golgi PI4P hydrolysis by PLCε to regulate cardiac hypertrophy. Elife. 2019 Aug 21;8. doi: 10.7554/eLife.48167. PubMed PMID: 31433293; PubMed Central PMCID: PMC6726460.
Gaylo-Moynihan A, Prizant H, Popović M, Fernandes NRJ, Anderson CS, Chiou KK, Bell H, Schrock DC, Schumacher J, Capece T, Walling BL, Topham DJ, Miller J, Smrcka AV, Kim M, Hughson A, Fowell DJ. Programming of Distinct Chemokine-Dependent and -Independent Search Strategies for Th1 and Th2 Cells Optimizes Function at Inflamed Sites. Immunity. 2019 Aug 20;51(2):298-309.e6. doi: 10.1016/j.immuni.2019.06.026. Epub 2019 Aug 6. PubMed PMID: 31399281; PubMed Central PMCID: PMC6904228.
Campbell AP, Smrcka AV. Targeting G protein-coupled receptor signalling by blocking G proteins. Nat Rev Drug Discov. 2018 Nov;17(11):789-803. doi: 10.1038/nrd.2018.135. Epub 2018 Sep 28. Review. PubMed PMID: 30262890; PubMed Central PMCID: PMC6409483.
de Rubio RG, Ransom RF, Malik S, Yule DI, Anantharam A, Smrcka AV. Phosphatidylinositol 4-phosphate is a major source of GPCR-stimulated phosphoinositide production. Sci Signal. 2018 Sep 11;11(547). doi: 10.1126/scisignal.aan1210. PubMed PMID: 30206135; PubMed Central PMCID: PMC6413320.
Nash CA, Brown LM, Malik S, Cheng X, Smrcka AV. Compartmentalized cyclic nucleotides have opposing effects on regulation of hypertrophic phospholipase Cε signaling in cardiac myocytes. J Mol Cell Cardiol. 2018 Aug;121:51-59. doi: 10.1016/j.yjmcc.2018.06.002. Epub 2018 Jun 7. PubMed PMID: 29885334; PubMed Central PMCID: PMC6373484.
Stoveken HM, Larsen SD, Smrcka AV, Tall GG. Gedunin- and Khivorin-Derivatives Are Small-Molecule Partial Agonists for Adhesion G Protein-Coupled Receptors GPR56/ADGRG1 and GPR114/ADGRG5. Mol Pharmacol. 2018 May;93(5):477-488. doi: 10.1124/mol.117.111476. Epub 2018 Feb 23. PubMed PMID: 29476042; PubMed Central PMCID: PMC5878671.
Madukwe JC, Garland-Kuntz EE, Lyon AM, Smrcka AV. G protein βγ subunits directly interact with and activate phospholipase Cϵ. J Biol Chem. 2018 Apr 27;293(17):6387-6397. doi: 10.1074/jbc.RA118.002354. Epub 2018 Mar 13. PubMed PMID: 29535186; PubMed Central PMCID: PMC5925795.
To JY, Smrcka AV. Activated heterotrimeric G protein αi subunits inhibit Rap-dependent cell adhesion and promote cell migration. J Biol Chem. 2018 Feb 2;293(5):1570-1578. doi: 10.1074/jbc.RA117.000964. Epub 2017 Dec 19. PubMed PMID: 29259127; PubMed Central PMCID: PMC5798289.
Kim KD, Bae S, Capece T, Nedelkovska H, de Rubio RG, Smrcka AV, Jun CD, Jung W, Park B, Kim TI, Kim M. Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment. Nat Commun. 2017 May 15;8:15365. doi: 10.1038/ncomms15365. PubMed PMID: 28504276; PubMed Central PMCID: PMC5440668.
Tong J, Liu X, Vickstrom C, Li Y, Yu L, Lu Y, Smrcka AV, Liu QS. The Epac-Phospholipase Cε Pathway Regulates Endocannabinoid Signaling and Cocaine-Induced Disinhibition of Ventral Tegmental Area Dopamine Neurons. J Neurosci. 2017 Mar 15;37(11):3030-3044. doi: 10.1523/JNEUROSCI.2810-16.2017. Epub 2017 Feb 16. PubMed PMID: 28209735; PubMed Central PMCID: PMC5354337.
Strazza M, Azoulay-Alfaguter I, Peled M, Smrcka AV, Skolnik EY, Srivastava S, Mor A. PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2693-2698. doi: 10.1073/pnas.1612900114. Epub 2017 Feb 17. PubMed PMID: 28213494; PubMed Central PMCID: PMC5347583.
DiStefano PV, Smrcka AV, Glading AJ. Phospholipase Cε Modulates Rap1 Activity and the Endothelial Barrier. PLoS One. 2016;11(9):e0162338. doi: 10.1371/journal.pone.0162338. eCollection 2016. PubMed PMID: 27612188; PubMed Central PMCID: PMC5017709.
Rangel-Moreno J, To JY, Owen T, Goldman BI, Smrcka AV, Anolik JH. Inhibition of G Protein βγ Subunit Signaling Abrogates Nephritis in Lupus-Prone Mice. Arthritis Rheumatol. 2016 Sep;68(9):2244-56. doi: 10.1002/art.39673. PubMed PMID: 26990948; PubMed Central PMCID: PMC5001921.
Stoveken HM, Bahr LL, Anders MW, Wojtovich AP, Smrcka AV, Tall GG. Dihydromunduletone Is a Small-Molecule Selective Adhesion G Protein-Coupled Receptor Antagonist. Mol Pharmacol. 2016 Sep;90(3):214-24. doi: 10.1124/mol.116.104828. Epub 2016 Jun 23. PubMed PMID: 27338081; PubMed Central PMCID: PMC4998661.
Bijli KM, Fazal F, Slavin SA, Leonard A, Grose V, Alexander WB, Smrcka AV, Rahman A. Phospholipase C-ε signaling mediates endothelial cell inflammation and barrier disruption in acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2016 Aug 1;311(2):L517-24. doi: 10.1152/ajplung.00069.2016. Epub 2016 Jul 1. PubMed PMID: 27371732; PubMed Central PMCID: PMC5243226.
Surve CR, To JY, Malik S, Kim M, Smrcka AV. Dynamic regulation of neutrophil polarity and migration by the heterotrimeric G protein subunits Gαi-GTP and Gβγ. Sci Signal. 2016 Feb 23;9(416):ra22. doi: 10.1126/scisignal.aad8163. PubMed PMID: 26905427; PubMed Central PMCID: PMC6364554.
Smrcka AV. Fingerprinting G protein-coupled receptor signaling. Sci Signal. 2015 Dec 1;8(405):fs20. doi: 10.1126/scisignal.aad8140. PubMed PMID: 26628679.
Dusaban SS, Kunkel MT, Smrcka AV, Brown JH. Thrombin promotes sustained signaling and inflammatory gene expression through the CDC25 and Ras-associating domains of phospholipase Cϵ. J Biol Chem. 2015 Oct 30;290(44):26776-83. doi: 10.1074/jbc.M115.676098. Epub 2015 Sep 8. PubMed PMID: 26350460; PubMed Central PMCID: PMC4646330.
Brand CS, Sadana R, Malik S, Smrcka AV, Dessauer CW. Adenylyl Cyclase 5 Regulation by Gβγ Involves Isoform-Specific Use of Multiple Interaction Sites. Mol Pharmacol. 2015 Oct;88(4):758-67. doi: 10.1124/mol.115.099556. Epub 2015 Jul 23. PubMed PMID: 26206488; PubMed Central PMCID: PMC4576683.
Kalwa H, Storch U, Demleitner J, Fiedler S, Mayer T, Kannler M, Fahlbusch M, Barth H, Smrcka A, Hildebrandt F, Gudermann T, Dietrich A. Phospholipase C epsilon (PLCε) induced TRPC6 activation: a common but redundant mechanism in primary podocytes. J Cell Physiol. 2015 Jun;230(6):1389-99. doi: 10.1002/jcp.24883. PubMed PMID: 25521631; PubMed Central PMCID: PMC4811033.
Malik S, deRubio RG, Trembley M, Irannejad R, Wedegaertner PB, Smrcka AV. G protein βγ subunits regulate cardiomyocyte hypertrophy through a perinuclear Golgi phosphatidylinositol 4-phosphate hydrolysis pathway. Mol Biol Cell. 2015 Mar 15;26(6):1188-98. doi: 10.1091/mbc.E14-10-1476. Epub 2015 Jan 21. PubMed PMID: 25609085; PubMed Central PMCID: PMC4357516.
Smrcka AV. Regulation of phosphatidylinositol-specific phospholipase C at the nuclear envelope in cardiac myocytes. J Cardiovasc Pharmacol. 2015 Mar;65(3):203-10. doi: 10.1097/FJC.0000000000000195. Review. PubMed PMID: 25658460; PubMed Central PMCID: PMC4355108.
Oldenburger A, Timens W, Bos S, Smit M, Smrcka AV, Laurent AC, Cao J, Hylkema M, Meurs H, Maarsingh H, Lezoualc'h F, Schmidt M. Epac1 and Epac2 are differentially involved in inflammatory and remodeling processes induced by cigarette smoke. FASEB J. 2014 Nov;28(11):4617-28. doi: 10.1096/fj.13-248930. Epub 2014 Aug 7. PubMed PMID: 25103224; PubMed Central PMCID: PMC4200332.
Le NT, Takei Y, Izawa-Ishizawa Y, Heo KS, Lee H, Smrcka AV, Miller BL, Ko KA, Ture S, Morrell C, Fujiwara K, Akaike M, Abe J. Identification of activators of ERK5 transcriptional activity by high-throughput screening and the role of endothelial ERK5 in vasoprotective effects induced by statins and antimalarial agents. J Immunol. 2014 Oct 1;193(7):3803-15. doi: 10.4049/jimmunol.1400571. Epub 2014 Sep 3. PubMed PMID: 25187658; PubMed Central PMCID: PMC4749510.
Surve CR, Lehmann D, Smrcka AV. A chemical biology approach demonstrates G protein βγ subunits are sufficient to mediate directional neutrophil chemotaxis. J Biol Chem. 2014 Jun 20;289(25):17791-801. doi: 10.1074/jbc.M114.576827. Epub 2014 May 7. PubMed PMID: 24808183; PubMed Central PMCID: PMC4067212.
Kamal FA, Mickelsen DM, Wegman KM, Travers JG, Moalem J, Hammes SR, Smrcka AV, Blaxall BC. Simultaneous adrenal and cardiac g-protein-coupled receptor-gβγ inhibition halts heart failure progression. J Am Coll Cardiol. 2014 Jun 17;63(23):2549-2557. doi: 10.1016/j.jacc.2014.02.587. Epub 2014 Apr 2. PubMed PMID: 24703913; PubMed Central PMCID: PMC4083020.
Kan W, Adjobo-Hermans M, Burroughs M, Faibis G, Malik S, Tall GG, Smrcka AV. M3 muscarinic receptor interaction with phospholipase C β3 determines its signaling efficiency. J Biol Chem. 2014 Apr 18;289(16):11206-18. doi: 10.1074/jbc.M113.538546. Epub 2014 Mar 4. PubMed PMID: 24596086; PubMed Central PMCID: PMC4036259.
Ruisanchez É, Dancs P, Kerék M, Németh T, Faragó B, Balogh A, Patil R, Jennings BL, Liliom K, Malik KU, Smrcka AV, Tigyi G, Benyó Z. Lysophosphatidic acid induces vasodilation mediated by LPA1 receptors, phospholipase C, and endothelial nitric oxide synthase. FASEB J. 2014 Feb;28(2):880-90. doi: 10.1096/fj.13-234997. Epub 2013 Nov 18. PubMed PMID: 24249637; PubMed Central PMCID: PMC3898652.
Xiang SY, Ouyang K, Yung BS, Miyamoto S, Smrcka AV, Chen J, Heller Brown J. PLCε, PKD1, and SSH1L transduce RhoA signaling to protect mitochondria from oxidative stress in the heart. Sci Signal. 2013 Dec 17;6(306):ra108. doi: 10.1126/scisignal.2004405. PubMed PMID: 24345679; PubMed Central PMCID: PMC4035240.
Easterhoff D, DiMaio JT, Liyanage W, Lo CW, Bae W, Doran TM, Smrcka A, Nilsson BL, Dewhurst S. Fluorescence detection of cationic amyloid fibrils in human semen. Bioorg Med Chem Lett. 2013 Sep 15;23(18):5199-202. doi: 10.1016/j.bmcl.2013.06.097. Epub 2013 Jul 8. PubMed PMID: 23910594; PubMed Central PMCID: PMC3895278.
Sun Z, Smrcka AV, Chen S. WDR26 functions as a scaffolding protein to promote Gβγ-mediated phospholipase C β2 (PLCβ2) activation in leukocytes. J Biol Chem. 2013 Jun 7;288(23):16715-25. doi: 10.1074/jbc.M113.462564. Epub 2013 Apr 26. PubMed PMID: 23625927; PubMed Central PMCID: PMC3675605.
Smrcka AV. Molecular targeting of Gα and Gβγ subunits: a potential approach for cancer therapeutics. Trends Pharmacol Sci. 2013 May;34(5):290-8. doi: 10.1016/j.tips.2013.02.006. Epub 2013 Apr 1. Review. PubMed PMID: 23557963; PubMed Central PMCID: PMC3924570.
Zhang L, Malik S, Pang J, Wang H, Park KM, Yule DI, Blaxall BC, Smrcka AV. Phospholipase Cε hydrolyzes perinuclear phosphatidylinositol 4-phosphate to regulate cardiac hypertrophy. Cell. 2013 Mar 28;153(1):216-27. doi: 10.1016/j.cell.2013.02.047. PubMed PMID: 23540699; PubMed Central PMCID: PMC3615249.
Dusaban SS, Purcell NH, Rockenstein E, Masliah E, Cho MK, Smrcka AV, Brown JH. Phospholipase C epsilon links G protein-coupled receptor activation to inflammatory astrocytic responses. Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3609-14. doi: 10.1073/pnas.1217355110. Epub 2013 Feb 11. PubMed PMID: 23401561; PubMed Central PMCID: PMC3587233.
Dbouk HA, Vadas O, Shymanets A, Burke JE, Salamon RS, Khalil BD, Barrett MO, Waldo GL, Surve C, Hsueh C, Perisic O, Harteneck C, Shepherd PR, Harden TK, Smrcka AV, Taussig R, Bresnick AR, Nürnberg B, Williams RL, Backer JM. G protein-coupled receptor-mediated activation of p110β by Gβγ is required for cellular transformation and invasiveness. Sci Signal. 2012 Dec 4;5(253):ra89. doi: 10.1126/scisignal.2003264. PubMed PMID: 23211529; PubMed Central PMCID: PMC3979326.
McCoy KL, Gyoneva S, Vellano CP, Smrcka AV, Traynelis SF, Hepler JR. Protease-activated receptor 1 (PAR1) coupling to G(q/11) but not to G(i/o) or G(12/13) is mediated by discrete amino acids within the receptor second intracellular loop. Cell Signal. 2012 Jun;24(6):1351-60. doi: 10.1016/j.cellsig.2012.01.011. Epub 2012 Jan 28. PubMed PMID: 22306780; PubMed Central PMCID: PMC3319227.
Smrcka AV, Brown JH, Holz GG. Role of phospholipase Cε in physiological phosphoinositide signaling networks. Cell Signal. 2012 Jun;24(6):1333-43. doi: 10.1016/j.cellsig.2012.01.009. Epub 2012 Jan 20. Review. PubMed PMID: 22286105; PubMed Central PMCID: PMC3325758.
Kamal FA, Smrcka AV, Blaxall BC. Taking the heart failure battle inside the cell: small molecule targeting of Gβγ subunits. J Mol Cell Cardiol. 2011 Oct;51(4):462-7. doi: 10.1016/j.yjmcc.2011.01.006. Epub 2011 Jan 21. Review. PubMed PMID: 21256851; PubMed Central PMCID: PMC3137754.
Lin Y, Smrcka AV. Understanding molecular recognition by G protein βγ subunits on the path to pharmacological targeting. Mol Pharmacol. 2011 Oct;80(4):551-7. doi: 10.1124/mol.111.073072. Epub 2011 Jul 7. Review. PubMed PMID: 21737569; PubMed Central PMCID: PMC3187535.
Seneviratne AM, Burroughs M, Giralt E, Smrcka AV. Direct-reversible binding of small molecules to G protein βγ subunits. Biochim Biophys Acta. 2011 Sep;1814(9):1210-8. doi: 10.1016/j.bbapap.2011.05.010. Epub 2011 May 18. PubMed PMID: 21621014; PubMed Central PMCID: PMC3140432.
Zhang L, Malik S, Kelley GG, Kapiloff MS, Smrcka AV. Phospholipase C epsilon scaffolds to muscle-specific A kinase anchoring protein (mAKAPbeta) and integrates multiple hypertrophic stimuli in cardiac myocytes. J Biol Chem. 2011 Jul 1;286(26):23012-21. doi: 10.1074/jbc.M111.231993. Epub 2011 May 5. PubMed PMID: 21550986; PubMed Central PMCID: PMC3123069.
Dzhura I, Chepurny OG, Leech CA, Roe MW, Dzhura E, Xu X, Lu Y, Schwede F, Genieser HG, Smrcka AV, Holz GG. Phospholipase C-ε links Epac2 activation to the potentiation of glucose-stimulated insulin secretion from mouse islets of Langerhans. Islets. 2011 May-Jun;3(3):121-8. doi: 10.4161/isl.3.3.15507. Epub 2011 May 1. PubMed PMID: 21478675; PubMed Central PMCID: PMC3116928.
Park MS, Smrcka AV, Stern HA. Conformational flexibility and binding interactions of the G protein βγ heterodimer. Proteins. 2011 Feb;79(2):518-27. doi: 10.1002/prot.22899. PubMed PMID: 21064128; PubMed Central PMCID: PMC3974715.
Chan P, Gabay M, Wright FA, Kan W, Oner SS, Lanier SM, Smrcka AV, Blumer JB, Tall GG. Purification of heterotrimeric G protein alpha subunits by GST-Ric-8 association: primary characterization of purified G alpha(olf). J Biol Chem. 2011 Jan 28;286(4):2625-35. doi: 10.1074/jbc.M110.178897. Epub 2010 Nov 29. PubMed PMID: 21115479; PubMed Central PMCID: PMC3024758.
Dessal AL, Prades R, Giralt E, Smrcka AV. Rational design of a selective covalent modifier of G protein βγ subunits. Mol Pharmacol. 2011 Jan;79(1):24-33. doi: 10.1124/mol.110.068155. Epub 2010 Sep 29. PubMed PMID: 20881007; PubMed Central PMCID: PMC3014279.
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