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Isoe J, Rascón AA Jr, Kunz S, Miesfeld RL. Molecular genetic analysis of midgut serine proteases in Aedes aegypti mosquitoes. Insect Biochem Mol Biol. 2009 Dec;39(12):903-12. doi: 10.1016/j.ibmb.2009.10.008. Epub 2009 Nov 3. PubMed PMID: 19883761; PubMed Central PMCID: PMC2818436.
Rascón AA Jr, Gearin J, Isoe J, Miesfeld RL. In vitro activation and enzyme kinetic analysis of recombinant midgut serine proteases from the Dengue vector mosquito Aedes aegypti. BMC Biochem. 2011 Aug 9;12:43. doi: 10.1186/1471-2091-12-43. PubMed PMID: 21827688; PubMed Central PMCID: PMC3162888.
Rascón AA Jr, McKerrow JH. Synthetic and natural protease inhibitors provide insights into parasite development, virulence and pathogenesis. Curr Med Chem. 2013;20(25):3078-102. doi: 10.2174/0929867311320250005. Review. PubMed PMID: 23514418.
Bulman CA, Bidlow CM, Lustigman S, Cho-Ngwa F, Williams D, Rascón AA Jr, Tricoche N, Samje M, Bell A, Suzuki B, Lim KC, Supakorndej N, Supakorndej P, Wolfe AR, Knudsen GM, Chen S, Wilson C, Ang KH, Arkin M, Gut J, Franklin C, Marcellino C, McKerrow JH, Debnath A, Sakanari JA. Repurposing auranofin as a lead candidate for treatment of lymphatic filariasis and onchocerciasis. PLoS Negl Trop Dis. 2015 Feb;9(2):e0003534. doi: 10.1371/journal.pntd.0003534. eCollection 2015 Feb. PubMed PMID: 25700363; PubMed Central PMCID: PMC4336141.
Long T, Rojo-Arreola L, Shi D, El-Sakkary N, Jarnagin K, Rock F, Meewan M, Rascón AA Jr, Lin L, Cunningham KA, Lemieux GA, Podust L, Abagyan R, Ashrafi K, McKerrow JH, Caffrey CR. Phenotypic, chemical and functional characterization of cyclic nucleotide phosphodiesterase 4 (PDE4) as a potential anthelmintic drug target. PLoS Negl Trop Dis. 2017 Jul;11(7):e0005680. doi: 10.1371/journal.pntd.0005680. eCollection 2017 Jul. PubMed PMID: 28704396; PubMed Central PMCID: PMC5526615.
Nguyen JT, Fong J, Fong D, Fong T, Lucero RM, Gallimore JM, Burata OE, Parungao K, Rascón AA Jr. Soluble expression of recombinant midgut zymogen (native propeptide) proteases from the Aedes aegypti Mosquito Utilizing E. coli as a host. BMC Biochem. 2018 Dec 18;19(1):12. doi: 10.1186/s12858-018-0101-0. PubMed PMID: 30563449; PubMed Central PMCID: PMC6299515.
Isoe J, Koch LE, Isoe YE, Rascón AA Jr, Brown HE, Massani BB, Miesfeld RL. Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes. PLoS Biol. 2019 Jan;17(1):e3000068. doi: 10.1371/journal.pbio.3000068. eCollection 2019 Jan. PubMed PMID: 30620728; PubMed Central PMCID: PMC6324781.
Isoe J, Simington CJ, Oscherwitz ME, Peterson AJ, Rascón AA Jr, Massani BB, Miesfeld RL, Riehle MA. Characterization of essential eggshell proteins from Aedes aegypti mosquitoes. BMC Biol. 2023 Oct 13;21(1):214. doi: 10.1186/s12915-023-01721-z. PubMed PMID: 37833714; PubMed Central PMCID: PMC10576393.
O'Donoghue AJ, Liu C, Simington CJ, Montermoso S, Moreno-Galvez E, Serafim MSM, Burata OE, Lucero RM, Nguyen JT, Fong D, Tran K, Millan N, Gallimore JM, Parungao K, Fong J, Suzuki BM, Jiang Z, Isoe J, Rascón AA Jr. Comprehensive proteolytic profiling of Aedes aegypti mosquito midgut extracts: Unraveling the blood meal protein digestion system. PLoS Negl Trop Dis. 2025 Feb;19(2):e0012555. doi: 10.1371/journal.pntd.0012555. eCollection 2025 Feb. PubMed PMID: 39913535; PubMed Central PMCID: PMC11838913.
Ramirez AG, Isoe J, Serafim MSM, Fong D, Le MA, Nguyen JT, Burata OE, Lucero RM, Spangler RK, Rascón AA Jr. Biochemical and physiological characterization of Aedes aegypti midgut chymotrypsin. Sci Rep. 2025 Mar 20;15(1):9685. doi: 10.1038/s41598-025-93413-7. PubMed PMID: 40113878; PubMed Central PMCID: PMC11926125.
Ramirez AG, Fong D, Le MA, Isoe J, Rascón AA Jr. Protocol for the detection of proteolytic activity in female Aedes aegypti mosquito midgut extracts using fluorogenic AMC protease substrates. STAR Protoc. 2025 Sep 19;6(3):103899. doi: 10.1016/j.xpro.2025.103899. Epub 2025 Jun 16. PubMed PMID: 40531626; PubMed Central PMCID: PMC12213286.
Rascón AA Jr. Improving the Capstone Biochemistry Lab and Evolution to a Course-Based Undergraduate Research Experience: Lessons Learned from the COVID-19 Online Modality. J Coll Sci Teach. 2025 Aug 4;. doi: 10.1080/0047231x.2025.2535961. [Epub ahead of print] PubMed PMID: 40823440; PubMed Central PMCID: PMC12356177.
Fong D, Millan N, Le MA, Moreno-Galvez E, Derisier K, Ramirez AG, Pluta K, Houghton K, Rascón AA Jr. Low Inducer Concentrations at 10°C Promotes Soluble Recombinant Expression of Aedes aegypti Mosquito Midgut Proteases in E. coli. Res Sq. 2025 Dec 11;. doi: 10.21203/rs.3.rs-8217026/v1. PubMed PMID: 41510259; PubMed Central PMCID: PMC12776528.
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