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Marichal-Gallardo P, Börner K, Pieler MM, Sonntag-Buck V, Obr M, Bejarano D, Wolff MW, Kräusslich HG, Reichl U, Grimm D. Single-Use Capture Purification of Adeno-Associated Viral Gene Transfer Vectors by Membrane-Based Steric Exclusion Chromatography. Hum Gene Ther. 2021 Mar 30;. doi: 10.1089/hum.2019.284. [Epub ahead of print] PubMed PMID: 33554722.
Hoffmann MD, Mathony J, Upmeier Zu Belzen J, Harteveld Z, Aschenbrenner S, Stengl C, Grimm D, Correia BE, Eils R, Niopek D. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. Nucleic Acids Res. 2021 Mar 18;49(5):e29. doi: 10.1093/nar/gkaa1198. PubMed PMID: 33330940; PubMed Central PMCID: PMC7969004.
Medert R, Jungmann A, Hildebrand S, Busch M, Grimm D, Flockerzi V, Müller OJ, Most P, Schumacher D, Freichel M. Development of an AAV9-RNAi-mediated silencing strategy to abrogate TRPM4 expression in the adult heart. Pflugers Arch. 2021 Mar;473(3):533-546. doi: 10.1007/s00424-021-02521-6. Epub 2021 Feb 13. PubMed PMID: 33580817; PubMed Central PMCID: PMC7940300.
Bubeck F, Grimm D. 'Hit and run' therapy averts macular degeneration. Nat Biomed Eng. 2021 Feb;5(2):132-133. doi: 10.1038/s41551-021-00690-4. PubMed PMID: 33580229.
El Andari J, Grimm D. Production, Processing, and Characterization of Synthetic AAV Gene Therapy Vectors. Biotechnol J. 2021 Jan;16(1):e2000025. doi: 10.1002/biot.202000025. Epub 2020 Oct 7. Review. PubMed PMID: 32975881.
Weinmann J, Weis S, Sippel J, Tulalamba W, Remes A, El Andari J, Herrmann AK, Pham QH, Borowski C, Hille S, Schönberger T, Frey N, Lenter M, VandenDriessche T, Müller OJ, Chuah MK, Lamla T, Grimm D. Identification of a myotropic AAV by massively parallel in vivo evaluation of barcoded capsid variants. Nat Commun. 2020 Oct 28;11(1):5432. doi: 10.1038/s41467-020-19230-w. PubMed PMID: 33116134; PubMed Central PMCID: PMC7595228.
Fakhiri J, Landegger LD, Grimm D. Breaking the sound barrier: Towards next-generation AAV vectors for gene therapy of hearing disorders. Hear Res. 2020 Oct 15;:108092. doi: 10.1016/j.heares.2020.108092. [Epub ahead of print] Review. PubMed PMID: 33268240.
Klein S, Müller TG, Khalid D, Sonntag-Buck V, Heuser AM, Glass B, Meurer M, Morales I, Schillak A, Freistaedter A, Ambiel I, Winter SL, Zimmermann L, Naumoska T, Bubeck F, Kirrmaier D, Ullrich S, Barreto Miranda I, Anders S, Grimm D, Schnitzler P, Knop M, Kräusslich HG, Dao Thi VL, Börner K, Chlanda P. SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP. Viruses. 2020 Aug 7;12(8). doi: 10.3390/v12080863. PubMed PMID: 32784757; PubMed Central PMCID: PMC7472728.
Mathony J, Harteveld Z, Schmelas C, Upmeier Zu Belzen J, Aschenbrenner S, Sun W, Hoffmann MD, Stengl C, Scheck A, Georgeon S, Rosset S, Wang Y, Grimm D, Eils R, Correia BE, Niopek D. Computational design of anti-CRISPR proteins with improved inhibition potency. Nat Chem Biol. 2020 Jul;16(7):725-730. doi: 10.1038/s41589-020-0518-9. Epub 2020 Apr 13. PubMed PMID: 32284602.
Fakhiri J, Linse KP, Mietzsch M, Xu M, Schneider MA, Meister M, Schildgen O, Schnitzler P, Soderlund-Venermo M, Agbandje-McKenna M, Grimm D. Impact of Natural or Synthetic Singletons in the Capsid of Human Bocavirus 1 on Particle Infectivity and Immunoreactivity. J Virol. 2020 May 18;94(11). doi: 10.1128/JVI.00170-20. Print 2020 May 18. PubMed PMID: 32213611; PubMed Central PMCID: PMC7269442.
Michler T, Kosinska AD, Festag J, Bunse T, Su J, Ringelhan M, Imhof H, Grimm D, Steiger K, Mogler C, Heikenwalder M, Michel ML, Guzman CA, Milstein S, Sepp-Lorenzino L, Knolle P, Protzer U. Knockdown of Virus Antigen Expression Increases Therapeutic Vaccine Efficacy in High-Titer Hepatitis B Virus Carrier Mice. Gastroenterology. 2020 May;158(6):1762-1775.e9. doi: 10.1053/j.gastro.2020.01.032. Epub 2020 Jan 28. PubMed PMID: 32001321.
Börner K, Kienle E, Huang LY, Weinmann J, Sacher A, Bayer P, Stüllein C, Fakhiri J, Zimmermann L, Westhaus A, Beneke J, Beil N, Wiedtke E, Schmelas C, Miltner D, Rau A, Erfle H, Kräusslich HG, Müller M, Agbandje-McKenna M, Grimm D. Pre-arrayed Pan-AAV Peptide Display Libraries for Rapid Single-Round Screening. Mol Ther. 2020 Apr 8;28(4):1016-1032. doi: 10.1016/j.ymthe.2020.02.009. Epub 2020 Feb 13. PubMed PMID: 32105604; PubMed Central PMCID: PMC7132618.
Tulalamba W, Weinmann J, Pham QH, El Andari J, VandenDriessche T, Chuah MK, Grimm D. Distinct transduction of muscle tissue in mice after systemic delivery of AAVpo1 vectors. Gene Ther. 2020 Apr;27(3-4):170-179. doi: 10.1038/s41434-019-0106-3. Epub 2019 Oct 17. PubMed PMID: 31624368.
Krooss SA, Dai Z, Schmidt F, Rovai A, Fakhiri J, Dhingra A, Yuan Q, Yang T, Balakrishnan A, Steinbrück L, Srivaratharajan S, Manns MP, Schambach A, Grimm D, Bohne J, Sharma AD, Büning H, Ott M. Ex Vivo/In vivo Gene Editing in Hepatocytes Using "All-in-One" CRISPR-Adeno-Associated Virus Vectors with a Self-Linearizing Repair Template. iScience. 2020 Jan 24;23(1):100764. doi: 10.1016/j.isci.2019.100764. Epub 2019 Dec 12. PubMed PMID: 31887661; PubMed Central PMCID: PMC6941859.
Hentzschel F, Mitesser V, Fraschka SA, Krzikalla D, Carrillo EH, Berkhout B, Bártfai R, Mueller AK, Grimm D. Gene knockdown in malaria parasites via non-canonical RNAi. Nucleic Acids Res. 2020 Jan 10;48(1):e2. doi: 10.1093/nar/gkz927. PubMed PMID: 31680162; PubMed Central PMCID: PMC7145648.
Haar J, Krämer C, Grimm D. Lab-Scale Production of Recombinant Adeno-Associated Viruses (AAV) for Expression of Optogenetic Elements. Methods Mol Biol. 2020;2173:83-100. doi: 10.1007/978-1-0716-0755-8_5. PubMed PMID: 32651911.
Eichhoff AM, Börner K, Albrecht B, Schäfer W, Baum N, Haag F, Körbelin J, Trepel M, Braren I, Grimm D, Adriouch S, Koch-Nolte F. Nanobody-Enhanced Targeting of AAV Gene Therapy Vectors. Mol Ther Methods Clin Dev. 2019 Dec 13;15:211-220. doi: 10.1016/j.omtm.2019.09.003. eCollection 2019 Dec 13. PubMed PMID: 31687421; PubMed Central PMCID: PMC6819893.
Domenger C, Grimm D. Next-generation AAV vectors-do not judge a virus (only) by its cover. Hum Mol Genet. 2019 Oct 1;28(R1):R3-R14. doi: 10.1093/hmg/ddz148. Review. PubMed PMID: 31261383.
Tabatabai J, Fakhiri J, Meyburg J, Linse KP, Xu M, Söderlund-Venermo M, Grimm D, Schnitzler P. Severe Human Bocavirus 1 Respiratory Tract Infection in an Immunodeficient Child With Fatal Outcome. Pediatr Infect Dis J. 2019 Sep;38(9):e219-e222. doi: 10.1097/INF.0000000000002354. PubMed PMID: 31033910.
Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Börner K, Eils R, Grimm D, Niopek D. Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Nucleic Acids Res. 2019 Jul 26;47(13):e75. doi: 10.1093/nar/gkz271. PubMed PMID: 30982889; PubMed Central PMCID: PMC6648350.
Fakhiri J, Schneider MA, Puschhof J, Stanifer M, Schildgen V, Holderbach S, Voss Y, El Andari J, Schildgen O, Boulant S, Meister M, Clevers H, Yan Z, Qiu J, Grimm D. Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses. Mol Ther Methods Clin Dev. 2019 Mar 15;12:202-222. doi: 10.1016/j.omtm.2019.01.003. eCollection 2019 Mar 15. PubMed PMID: 30766894; PubMed Central PMCID: PMC6360332.
Herrmann AK, Bender C, Kienle E, Grosse S, El Andari J, Botta J, Schürmann N, Wiedtke E, Niopek D, Grimm D. A Robust and All-Inclusive Pipeline for Shuffling of Adeno-Associated Viruses. ACS Synth Biol. 2019 Jan 18;8(1):194-206. doi: 10.1021/acssynbio.8b00373. Epub 2019 Jan 8. PubMed PMID: 30513195.
Fanucchi S, Fok ET, Dalla E, Shibayama Y, Börner K, Chang EY, Stoychev S, Imakaev M, Grimm D, Wang KC, Li G, Sung WK, Mhlanga MM. Immune genes are primed for robust transcription by proximal long noncoding RNAs located in nuclear compartments. Nat Genet. 2019 Jan;51(1):138-150. doi: 10.1038/s41588-018-0298-2. Epub 2018 Dec 10. PubMed PMID: 30531872.
Herrmann AK, Große S, Börner K, Krämer C, Wiedtke E, Gunkel M, Grimm D. Impact of the Assembly-Activating Protein on Molecular Evolution of Synthetic Adeno-Associated Virus Capsids. Hum Gene Ther. 2019 Jan;30(1):21-35. doi: 10.1089/hum.2018.085. Epub 2018 Dec 18. PubMed PMID: 29978729.
Wolff G, Taranko AE, Meln I, Weinmann J, Sijmonsma T, Lerch S, Heide D, Billeter AT, Tews D, Krunic D, Fischer-Posovszky P, Müller-Stich BP, Herzig S, Grimm D, Heikenwälder M, Kao WW, Vegiopoulos A. Diet-dependent function of the extracellular matrix proteoglycan Lumican in obesity and glucose homeostasis. Mol Metab. 2019 Jan;19:97-106. doi: 10.1016/j.molmet.2018.10.007. Epub 2018 Oct 23. PubMed PMID: 30409703; PubMed Central PMCID: PMC6323191.
Fakhiri J, Nickl M, Grimm D. Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors. Methods Mol Biol. 2019;1961:111-126. doi: 10.1007/978-1-4939-9170-9_8. PubMed PMID: 30912043.
Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods. 2018 Nov;15(11):924-927. doi: 10.1038/s41592-018-0178-9. Epub 2018 Oct 30. PubMed PMID: 30377362.
Schmelas C, Grimm D. Split Cas9, Not Hairs - Advancing the Therapeutic Index of CRISPR Technology. Biotechnol J. 2018 Sep;13(9):e1700432. doi: 10.1002/biot.201700432. Epub 2018 Feb 2. Review. PubMed PMID: 29316283.
Herrmann AK, Grimm D. High-Throughput Dissection of AAV-Host Interactions: The Fast and the Curious. J Mol Biol. 2018 Aug 17;430(17):2626-2640. doi: 10.1016/j.jmb.2018.05.022. Epub 2018 May 19. Review. PubMed PMID: 29782834.
Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, Fronza R, Landerer H, Abad M, Niopek D, Schmidt M, Serrano M, Grimm D. AAVvector-mediated in vivo reprogramming into pluripotency. Nat Commun. 2018 Jul 9;9(1):2651. doi: 10.1038/s41467-018-05059-x. PubMed PMID: 29985406; PubMed Central PMCID: PMC6037684.
Cordas Dos Santos DM, Eilers J, Sosa Vizcaino A, Orlova E, Zimmermann M, Stanulla M, Schrappe M, Börner K, Grimm D, Muckenthaler MU, Kulozik AE, Kunz JB. MAP3K7 is recurrently deleted in pediatric T-lymphoblastic leukemia and affects cell proliferation independently of NF-κB. BMC Cancer. 2018 Jun 18;18(1):663. doi: 10.1186/s12885-018-4525-0. PubMed PMID: 29914415; PubMed Central PMCID: PMC6006985.
Kunze C, Börner K, Kienle E, Orschmann T, Rusha E, Schneider M, Radivojkov-Blagojevic M, Drukker M, Desbordes S, Grimm D, Brack-Werner R. Synthetic AAV/CRISPR vectors for blocking HIV-1 expression in persistently infected astrocytes. Glia. 2018 Feb;66(2):413-427. doi: 10.1002/glia.23254. Epub 2017 Nov 9. PubMed PMID: 29119608.
Lou WP, Mateos A, Koch M, Klussman S, Yang C, Lu N, Kumar S, Limpert S, Göpferich M, Zschaetzsch M, Sliwinski C, Kenzelmann M, Seedorf M, Maillo C, Senis E, Grimm D, Puttagunta R, Mendez R, Liu K, Hassan BA, Martin-Villalba A. Regulation of Adult CNS Axonal Regeneration by the Post-transcriptional Regulator Cpeb1. Front Mol Neurosci. 2017;10:445. doi: 10.3389/fnmol.2017.00445. eCollection 2017. PubMed PMID: 29379413; PubMed Central PMCID: PMC5770975.
Amoasii L, Long C, Li H, Mireault AA, Shelton JM, Sanchez-Ortiz E, McAnally JR, Bhattacharyya S, Schmidt F, Grimm D, Hauschka SD, Bassel-Duby R, Olson EN. Single-cut genome editing restores dystrophin expression in a new mouse model of muscular dystrophy. Sci Transl Med. 2017 Nov 29;9(418). doi: 10.1126/scitranslmed.aan8081. PubMed PMID: 29187645; PubMed Central PMCID: PMC5749406.
Grimm D, Büning H. Small But Increasingly Mighty: Latest Advances in AAV Vector Research, Design, and Evolution. Hum Gene Ther. 2017 Nov;28(11):1075-1086. doi: 10.1089/hum.2017.172. Epub 2017 Aug 23. Review. PubMed PMID: 28835125.
Grosse S, Penaud-Budloo M, Herrmann AK, Börner K, Fakhiri J, Laketa V, Krämer C, Wiedtke E, Gunkel M, Ménard L, Ayuso E, Grimm D. Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells. J Virol. 2017 Oct 15;91(20). doi: 10.1128/JVI.01198-17. Print 2017 Oct 15. PubMed PMID: 28768875; PubMed Central PMCID: PMC5625497.
Weinmann J, Grimm D. Next-generation AAV vectors for clinical use: an ever-accelerating race. Virus Genes. 2017 Oct;53(5):707-713. doi: 10.1007/s11262-017-1502-7. Epub 2017 Jul 31. Review. PubMed PMID: 28762205.
Lempp FA, Wiedtke E, Qu B, Roques P, Chemin I, Vondran FWR, Le Grand R, Grimm D, Urban S. Sodium taurocholate cotransporting polypeptide is the limiting host factor of hepatitis B virus infection in macaque and pig hepatocytes. Hepatology. 2017 Sep;66(3):703-716. doi: 10.1002/hep.29112. Epub 2017 Jul 18. PubMed PMID: 28195359.
Pervolaraki K, Stanifer ML, Münchau S, Renn LA, Albrecht D, Kurzhals S, Senís E, Grimm D, Schröder-Braunstein J, Rabin RL, Boulant S. Type I and Type III Interferons Display Different Dependency on Mitogen-Activated Protein Kinases to Mount an Antiviral State in the Human Gut. Front Immunol. 2017;8:459. doi: 10.3389/fimmu.2017.00459. eCollection 2017. PubMed PMID: 28484457; PubMed Central PMCID: PMC5399069.
Sá E Cunha C, Nyboer B, Heiss K, Sanches-Vaz M, Fontinha D, Wiedtke E, Grimm D, Przyborski JM, Mota MM, Prudêncio M, Mueller AK. Plasmodium berghei EXP-1 interacts with host Apolipoprotein H during Plasmodium liver-stage development. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1138-E1147. doi: 10.1073/pnas.1606419114. Epub 2017 Jan 30. PubMed PMID: 28137845; PubMed Central PMCID: PMC5320984.
Senís E, Mockenhaupt S, Rupp D, Bauer T, Paramasivam N, Knapp B, Gronych J, Grosse S, Windisch MP, Schmidt F, Theis FJ, Eils R, Lichter P, Schlesner M, Bartenschlager R, Grimm D. TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus. Nucleic Acids Res. 2017 Jan 9;45(1):e3. doi: 10.1093/nar/gkw805. Epub 2016 Sep 9. PubMed PMID: 27614072; PubMed Central PMCID: PMC5224498.
Michler T, Große S, Mockenhaupt S, Röder N, Stückler F, Knapp B, Ko C, Heikenwalder M, Protzer U, Grimm D. Blocking sense-strand activity improves potency, safety and specificity of anti-hepatitis B virus short hairpin RNA. EMBO Mol Med. 2016 Sep;8(9):1082-98. doi: 10.15252/emmm.201506172. Print 2016 Sep. PubMed PMID: 27473329; PubMed Central PMCID: PMC5009812.
Ebrahimi-Fakhari D, Wahlster L, Bartz F, Werenbeck-Ueding J, Praggastis M, Zhang J, Joggerst-Thomalla B, Theiss S, Grimm D, Ory DS, Runz H. Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells. Hum Mol Genet. 2016 Aug 15;25(16):3588-3599. doi: 10.1093/hmg/ddw204. Epub 2016 Jul 4. PubMed PMID: 27378690; PubMed Central PMCID: PMC5179952.
Pujol FM, Laketa V, Schmidt F, Mukenhirn M, Müller B, Boulant S, Grimm D, Keppler OT, Fackler OT. HIV-1 Vpu Antagonizes CD317/Tetherin by Adaptor Protein-1-Mediated Exclusion from Virus Assembly Sites. J Virol. 2016 Aug 1;90(15):6709-6723. doi: 10.1128/JVI.00504-16. Print 2016 Aug 1. PubMed PMID: 27170757; PubMed Central PMCID: PMC4944280.
Hentzschel F, Herrmann AK, Mueller AK, Grimm D. Plasmodium meets AAV-the (un)likely marriage of parasitology and virology, and how to make the match. FEBS Lett. 2016 Jul;590(13):2027-45. doi: 10.1002/1873-3468.12187. Epub 2016 May 9. Review. PubMed PMID: 27117587.
Rezvani M, Español-Suñer R, Malato Y, Dumont L, Grimm AA, Kienle E, Bindman JG, Wiedtke E, Hsu BY, Naqvi SJ, Schwabe RF, Corvera CU, Grimm D, Willenbring H. In Vivo Hepatic Reprogramming of Myofibroblasts with AAV Vectors as a Therapeutic Strategy for Liver Fibrosis. Cell Stem Cell. 2016 Jun 2;18(6):809-816. doi: 10.1016/j.stem.2016.05.005. PubMed PMID: 27257763; PubMed Central PMCID: PMC5325707.
Liesche C, Venkatraman L, Aschenbrenner S, Grosse S, Grimm D, Eils R, Beaudouin J. Death receptor-based enrichment of Cas9-expressing cells. BMC Biotechnol. 2016 Feb 16;16:17. doi: 10.1186/s12896-016-0250-4. PubMed PMID: 26883910; PubMed Central PMCID: PMC4755021.
Grimm D, Zolotukhin S. E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal--Tailored Acceleration of AAV Evolution. Mol Ther. 2015 Dec;23(12):1819-31. doi: 10.1038/mt.2015.173. Epub 2015 Sep 21. Review. PubMed PMID: 26388463; PubMed Central PMCID: PMC4700111.
Singer M, Marshall J, Heiss K, Mair GR, Grimm D, Mueller AK, Frischknecht F. Zinc finger nuclease-based double-strand breaks attenuate malaria parasites and reveal rare microhomology-mediated end joining. Genome Biol. 2015 Nov 17;16:249. doi: 10.1186/s13059-015-0811-1. PubMed PMID: 26573820; PubMed Central PMCID: PMC4647826.
Mockenhaupt S, Grosse S, Rupp D, Bartenschlager R, Grimm D. Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys. Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):E4007-16. doi: 10.1073/pnas.1510476112. Epub 2015 Jul 13. PubMed PMID: 26170322; PubMed Central PMCID: PMC4522753.
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