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Rafalowski A, Hassan BA, Lou K, Nguyen MC, Taylor EA. How Single Amino Acid Substitutions Can Disrupt a Protein Hetero-Dimer Interface: Computational and Experimental Studies of the LigAB Dioxygenase from Sphingobium sp. Strain SYK-6. Int J Mol Sci. 2023 Mar 28;24(7). doi: 10.3390/ijms24076319. PubMed PMID: 37047291; PubMed Central PMCID: PMC10094722.
Hassan BA, Liu ZA, Milicaj J, Kim MS, Tyson M, Sham YY, Taylor EA. Kinetic Characterization and Computational Modeling of Escherichia coli Heptosyltransferase II: Exploring the Role of Protein Dynamics in Catalysis for GT-B Glycosyltransferase. Biochemistry. 2022 Aug 2;61(15):1572-1584. doi: 10.1021/acs.biochem.2c00329. Epub 2022 Jul 21. PubMed PMID: 35861590.
Hassan B, Liu Z, Milicaj J, Kim M, Tyson M, Sham Y, Taylor E. Kinetic Characterization and Computational Modeling of the <i>Escherichia coli</i> Heptosyltransferase II: Exploring the Role of Protein Dynamics in Catalysis for a GT-B Glycosyltransferase. [preprint]. 2022 June. doi: 10.1101/2022.06.13.495986.
Milicaj J, Hassan BA, Cote JM, Ramirez-Mondragon CA, Jaunbocus N, Rafalowski A, Patel KR, Castro CD, Muthyala R, Sham YY, Taylor EA. Discovery of first-in-class nanomolar inhibitors of heptosyltransferase I reveals a new aminoglycoside target and potential alternative mechanism of action. Sci Rep. 2022 May 4;12(1):7302. doi: 10.1038/s41598-022-10776-x. PubMed PMID: 35508636; PubMed Central PMCID: PMC9068772.
Milicaj J, Dodda V, Patel K, Aragon I, O’Connell T, Muthyala R, Taylor E, Sham Y. Facile and Adaptable Synthesis of a Prazosin Analogue Library: Bringing Medicinal Chemistry into the Undergraduate Curriculum. Journal of Chemical Education. 2022 February; 99(3):1428-1434. doi: 10.1021/acs.jchemed.1c00822.
Hassan BA, Milicaj J, Ramirez-Mondragon CA, Sham YY, Taylor EA. Ligand-Induced Conformational and Dynamical Changes in a GT-B Glycosyltransferase: Molecular Dynamics Simulations of Heptosyltransferase I Complexes. J Chem Inf Model. 2022 Jan 24;62(2):324-339. doi: 10.1021/acs.jcim.1c00868. Epub 2021 Dec 30. PubMed PMID: 34967618; PubMed Central PMCID: PMC8864558.
Shukla N, Bembenek B, Taylor EA, Othon CM. Conformational Consequences for Compatible Osmolytes on Thermal Denaturation. Life (Basel). 2021 Dec 13;11(12). doi: 10.3390/life11121394. PubMed PMID: 34947925; PubMed Central PMCID: PMC8708791.
Milicaj J, Hassan B, Cote J, Ramirez-Mondragon C, Jaunbocus N, Rafalowski A, Patel K, Muthyala R, Sham Y, Taylor E. Discovery of Aminoglycosides as first in class, nanomolar inhibitors of Heptosyltransferase I. [preprint]. 2021 October. doi: 10.1101/2021.10.03.462930.
Milicaj J, Castro C, Jaunbocus N, Taylor E. Extraction of ADP-Heptose and Kdo2-Lipid A from E. coli Deficient in the Heptosyltransferase I Gene. Applied Sciences. 2021 September; 11(18):8314-. doi: 10.3390/app11188314.
Ramirez-Mondragon CA, Nguyen ME, Milicaj J, Hassan BA, Tucci FJ, Muthyala R, Gao J, Taylor EA, Sham YY. Conserved Conformational Hierarchy across Functionally Divergent Glycosyltransferases of the GT-B Structural Superfamily as Determined from Microsecond Molecular Dynamics. Int J Mol Sci. 2021 Apr 28;22(9). doi: 10.3390/ijms22094619. PubMed PMID: 33924837; PubMed Central PMCID: PMC8124905.
Li T, Tikad A, Fu H, Milicaj J, Castro CD, Lacritick M, Pan W, Taylor EA, Vincent SP. A General Strategy to Synthesize ADP-7-Azido-heptose and ADP-Azido-mannoses and Their Heptosyltransferase Binding Properties. Org Lett. 2021 Mar 5;23(5):1638-1642. doi: 10.1021/acs.orglett.1c00048. Epub 2021 Feb 23. PubMed PMID: 33620227.
Uchendu SN, Rafalowski A, Schick DL, Cohn EF, Hassan BA, Taylor EA. Identifying Metabolic Pathway Intermediates that Modulate Enzyme Activity: A Kinetic Analysis of the DesB Dioxygenase from Sphingobium sp. strain SYK-6. Process Biochemistry. 2021 March; 102:408-416. doi:
Cote JM, Hecht CJS, Patel KR, Ramirez-Mondragon CA, Sham YY, Taylor EA. Opposites Attract: Escherichia coli Heptosyltransferase I Conformational Changes Induced by Interactions between the Substrate and Positively Charged Residues. Biochemistry. 2020 Sep 1;59(34):3135-3147. doi: 10.1021/acs.biochem.9b01005. Epub 2020 Feb 10. PubMed PMID: 32011131; PubMed Central PMCID: PMC7582807.
Ramirez-Mondragon C, Nguyen M, Milicaj J, Tucci F, Muthyala R, Taylor E, Sham Y. Conserved conformational hierarchy across functionally divergent glycosyltransferases of the GT-B structural superfamily as determined from microsecond molecular dynamics. [preprint]. 2020 July. doi: 10.1101/2020.07.02.181073.
Taylor EA. Lignin Enzymology – Recent Efforts to Understand Lignin Monomer Catabolism. In: Begley TP, Liu H, editors. Comprehensive Natural Products III 3 ed. Oxford: Elsevier; 2020. p.373-398. 4238p.
Shukla N, Goeks J, Taylor EA, Othon CM. Hydration Dynamics in Solutions of Cyclic Polyhydroxyl Osmolytes. J Phys Chem B. 2019 Oct 10;123(40):8472-8479. doi: 10.1021/acs.jpcb.9b06861. Epub 2019 Oct 1. PubMed PMID: 31508961.
Henry RA, Kuo YM, Siegel ZS, Yen TJ, Rhodes J, Taylor EA, Andrews AJ. Discordant Effects of Putative Lysine Acetyltransferase Inhibitors in Biochemical and Living Systems. Cells. 2019 Sep 2;8(9). doi: 10.3390/cells8091022. PubMed PMID: 31480793; PubMed Central PMCID: PMC6770547.
Burroughs AM, Glasner ME, Barry KP, Taylor EA, Aravind L. Oxidative opening of the aromatic ring: Tracing the natural history of a large superfamily of dioxygenase domains and their relatives. J Biol Chem. 2019 Jun 28;294(26):10211-10235. doi: 10.1074/jbc.RA119.007595. Epub 2019 May 15. PubMed PMID: 31092555; PubMed Central PMCID: PMC6664185.
Uchendu SN, Rafalowski A, Cohn EF, Davoren LW, Taylor EA. Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition. J Vis Exp. 2018 Oct 3;(140). doi: 10.3791/58307. PubMed PMID: 30346405; PubMed Central PMCID: PMC6235412.
Nkosana NK, Czyzyk DJ, Siegel ZS, Cote JM, Taylor EA. Synthesis, kinetics and inhibition of Escherichia coli Heptosyltransferase I by monosaccharide analogues of Lipid A. Bioorg Med Chem Lett. 2018 Feb 15;28(4):594-600. doi: 10.1016/j.bmcl.2018.01.040. Epub 2018 Feb 2. PubMed PMID: 29398539; PubMed Central PMCID: PMC5885750.
Shukla N, Pomarico E, Hecht CJS, Taylor EA, Chergui M, Othon CM. Hydrophobic interactions of sucralose with protein structures. Arch Biochem Biophys. 2018 Feb 1;639:38-43. doi: 10.1016/ Epub 2017 Dec 26. PubMed PMID: 29288052.
Cote JM, Taylor EA. The Glycosyltransferases of LPS Core: A Review of Four Heptosyltransferase Enzymes in Context. Int J Mol Sci. 2017 Oct 27;18(11). doi: 10.3390/ijms18112256. Review. PubMed PMID: 29077008; PubMed Central PMCID: PMC5713226.
Cote JM, Ramirez-Mondragon CA, Siegel ZS, Czyzyk DJ, Gao J, Sham YY, Mukerji I, Taylor EA. The Stories Tryptophans Tell: Exploring Protein Dynamics of Heptosyltransferase I from Escherichia coli. Biochemistry. 2017 Feb 14;56(6):886-895. doi: 10.1021/acs.biochem.6b00850. Epub 2017 Jan 30. PubMed PMID: 28098447; PubMed Central PMCID: PMC5546316.
Sefcikova Chandrashekar C, Shellikeri A, Chandrashekar S, Taylor EA, Taylor DM. Visualizing electromagnetic fields in metals by MRI. AIP advances. 2017 February; 7. doi:
Chandrashekar C, Shellikeri A, Chandrashekar S, Taylor E, Taylor D. Visualizing Electromagnetic Vacuum by MRI. [preprint]. 2016 December. doi: 10.20944/preprints201610.0042.v3.
Chandrashekar C, Shellikeri A, Chandrashekar S, Taylor E, Taylor D. Visualizing Electromagnetic Vacuum by MRI. [preprint]. 2016 November. doi: 10.20944/preprints201610.0042.v2.
Chandrashekar C, Shellikeri A, Chandrashekar S, Taylor E, Taylor D. Visualizing Electromagnetic Vacuum by MRI. [preprint]. 2016 October. doi: 10.20944/preprints201610.0042.v1.
Christian T, Sakaguchi R, Perlinska AP, Lahoud G, Ito T, Taylor EA, Yokoyama S, Sulkowska JI, Hou YM. Methyl transfer by substrate signaling from a knotted protein fold. Nat Struct Mol Biol. 2016 Oct;23(10):941-948. doi: 10.1038/nsmb.3282. Epub 2016 Aug 29. PubMed PMID: 27571175; PubMed Central PMCID: PMC5429141.
Barry KP, Cohn EF, Ngu A, Taylor EA. Improving Alternate Lignin Catabolite Utilization of LigAB from Sphingobium sp. strain SYK-6 through Site Directed Mutagenesis. Process biochemistry (Barking, London, England). 2015 October; 50:1634-1639. doi: 10.1016/j.procbio.2015.05.024.
Mudapaka J, Taylor EA. Cloning and characterization of the Escherichia coli Heptosyltransferase III: Exploring substrate specificity in lipopolysaccharide core biosynthesis. FEBS Lett. 2015 Jun 4;589(13):1423-9. doi: 10.1016/j.febslet.2015.04.051. Epub 2015 May 7. PubMed PMID: 25957775.
Chen L, Shukla N, Cho I, Cohn E, Taylor EA, Othon CM. Sucralose Destabilization of Protein Structure. J Phys Chem Lett. 2015 Apr 16;6(8):1441-6. doi: 10.1021/acs.jpclett.5b00442. Epub 2015 Apr 2. PubMed PMID: 26263149.
Barry KP, Ngu A, Cohn EF, Cote JM, Burroughs AM, Gerbino JP, Taylor EA. Exploring allosteric activation of LigAB from Sphingobium sp. strain SYK-6 through kinetics, mutagenesis and computational studies. Arch Biochem Biophys. 2015 Feb 1;567:35-45. doi: 10.1016/ Epub 2015 Jan 3. PubMed PMID: 25562402.
Barry KP, Taylor EA. Characterizing the promiscuity of LigAB, a lignin catabolite degrading extradiol dioxygenase from Sphingomonas paucimobilis SYK-6. Biochemistry. 2013 Sep 24;52(38):6724-36. doi: 10.1021/bi400665t. Epub 2013 Sep 11. PubMed PMID: 23977959; PubMed Central PMCID: PMC3926093.
Czyzyk DJ, Sawant SS, Ramirez-Mondragon CA, Hingorani MM, Taylor EA. Escherichia coli heptosyltransferase I: investigation of protein dynamics of a GT-B structural enzyme. Biochemistry. 2013 Aug 6;52(31):5158-60. doi: 10.1021/bi400807r. Epub 2013 Jul 23. PubMed PMID: 23865375; PubMed Central PMCID: PMC3867311.
Czyzyk DJ, Liu C, Taylor EA. Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I. Biochemistry. 2011 Dec 13;50(49):10570-2. doi: 10.1021/bi201581b. Epub 2011 Nov 15. PubMed PMID: 22059588; PubMed Central PMCID: PMC3263931.
Czyzyk, Daniel J., Taylor, Erika A.. Heptosyltransferase I Lipid a Structure Activity Relationships (SAR). Biophysical Journal. 2011; 100(3):219.
Li L, Luo M, Ghanem M, Taylor EA, Schramm VL. Second-sphere amino acids contribute to transition-state structure in bovine purine nucleoside phosphorylase. Biochemistry. 2008 Feb 26;47(8):2577-83. doi: 10.1021/bi7021365. PubMed PMID: 18281958.
Taylor EA, Rinaldo-Matthis A, Li L, Ghanem M, Hazleton KZ, Cassera MB, Almo SC, Schramm VL. Anopheles gambiae purine nucleoside phosphorylase: catalysis, structure, and inhibition. Biochemistry. 2007 Oct 30;46(43):12405-15. doi: 10.1021/bi7010256. Epub 2007 Oct 6. PubMed PMID: 17918964.
Luo M, Singh V, Taylor EA, Schramm VL. Transition-state variation in human, bovine, and Plasmodium falciparum adenosine deaminases. J Am Chem Soc. 2007 Jun 27;129(25):8008-17. doi: 10.1021/ja072122y. Epub 2007 May 31. PubMed PMID: 17536804; PubMed Central PMCID: PMC2522313.
Taylor EA, Clinch K, Kelly PM, Li L, Evans GB, Tyler PC, Schramm VL. Acyclic ribooxacarbenium ion mimics as transition state analogues of human and malarial purine nucleoside phosphorylases. J Am Chem Soc. 2007 Jun 6;129(22):6984-5. doi: 10.1021/ja071087s. Epub 2007 May 11. PubMed PMID: 17497780.
Tyler PC, Taylor EA, Fröhlich RF, Schramm VL. Synthesis of 5'-methylthio coformycins: specific inhibitors for malarial adenosine deaminase. J Am Chem Soc. 2007 May 30;129(21):6872-9. doi: 10.1021/ja0708363. Epub 2007 May 8. PubMed PMID: 17488013; PubMed Central PMCID: PMC2522312.
Murkin AS, Birck MR, Rinaldo-Matthis A, Shi W, Taylor EA, Almo SC, Schramm VL. Neighboring group participation in the transition state of human purine nucleoside phosphorylase. Biochemistry. 2007 May 1;46(17):5038-49. doi: 10.1021/bi700147b. Epub 2007 Apr 4. PubMed PMID: 17407325; PubMed Central PMCID: PMC2526054.
Taylor Ringia EA, Tyler PC, Evans GB, Furneaux RH, Murkin AS, Schramm VL. Transition state analogue discrimination by related purine nucleoside phosphorylases. J Am Chem Soc. 2006 Jun 7;128(22):7126-7. doi: 10.1021/ja061403n. PubMed PMID: 16734442; PubMed Central PMCID: PMC2518100.
Clinch K, Evans GB, Fleet GW, Furneaux RH, Johnson SW, Lenz DH, Mee SP, Rands PR, Schramm VL, Taylor Ringia EA, Tyler PC. Syntheses and bio-activities of the L-enantiomers of two potent transition state analogue inhibitors of purine nucleoside phosphorylases. Org Biomol Chem. 2006 Mar 21;4(6):1131-9. doi: 10.1039/b517883e. Epub 2006 Jan 26. PubMed PMID: 16525558.
Fridman, Frida, Ringia, Erika A. Taylor, Schramm, Vern L.. Inhibitor screening for human purine nucleoside phosphorylase, bovine xanthine oxidase and E. coli thymidine phosphorylase. Abstracts of Papers of the American Chemical Society. 2006; 231.
Lewandowicz A, Ringia EA, Ting LM, Kim K, Tyler PC, Evans GB, Zubkova OV, Mee S, Painter GF, Lenz DH, Furneaux RH, Schramm VL. Energetic mapping of transition state analogue interactions with human and Plasmodium falciparum purine nucleoside phosphorylases. J Biol Chem. 2005 Aug 26;280(34):30320-8. doi: 10.1074/jbc.M505033200. Epub 2005 Jun 16. PubMed PMID: 15961383.
Ting LM, Shi W, Lewandowicz A, Singh V, Mwakingwe A, Birck MR, Ringia EA, Bench G, Madrid DC, Tyler PC, Evans GB, Furneaux RH, Schramm VL, Kim K. Targeting a novel Plasmodium falciparum purine recycling pathway with specific immucillins. J Biol Chem. 2005 Mar 11;280(10):9547-54. doi: 10.1074/jbc.M412693200. Epub 2004 Dec 2. PubMed PMID: 15576366.
Taylor Ringia EA, Schramm VL. Transition states and inhibitors of the purine nucleoside phosphorylase family. Curr Top Med Chem. 2005;5(13):1237-58. doi: 10.2174/156802605774463088. Review. PubMed PMID: 16305529.
Thoden JB, Taylor Ringia EA, Garrett JB, Gerlt JA, Holden HM, Rayment I. Evolution of enzymatic activity in the enolase superfamily: structural studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis. Biochemistry. 2004 May 18;43(19):5716-27. doi: 10.1021/bi0497897. PubMed PMID: 15134446.
Taylor Ringia EA, Garrett JB, Thoden JB, Holden HM, Rayment I, Gerlt JA. Evolution of enzymatic activity in the enolase superfamily: functional studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis. Biochemistry. 2004 Jan 13;43(1):224-9. doi: 10.1021/bi035815+. PubMed PMID: 14705949.
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