My Bibliography

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Fenton BA, Tomberg J, Sciandra CA, Nicholas RA, Davies C, Zhou P. Mutations in PBP2 from ceftriaxone-resistant Neisseria gonorrhoeae alter the dynamics of the β3-β4 loop to favor a low-affinity drug-binding state. J Biol Chem. 2021 Oct;297(4):101188. doi: 10.1016/j.jbc.2021.101188. Epub 2021 Sep 13. PubMed PMID: 34529975; PubMed Central PMCID: PMC8503634.

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Murphy TC, Mortimer TD, Nicholas RA, Wadsworth CB. Draft Genome Sequences of Three Penicillin-Resistant Neisseria gonorrhoeae Strains Isolated in Cincinnati, Ohio, in 1994. Microbiol Resour Announc. 2021 Mar 18;10(11). doi: 10.1128/MRA.00074-21. PubMed PMID: 33737358; PubMed Central PMCID: PMC7975876.

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Turner JM, Connolly KL, Aberman KE, Fonseca JC 2nd, Singh A, Jerse AE, Nicholas RA, Davies C. Molecular Features of Cephalosporins Important for Activity against Antimicrobial-Resistant Neisseria gonorrhoeae. ACS Infect Dis. 2021 Feb 12;7(2):293-308. doi: 10.1021/acsinfecdis.0c00400. Epub 2021 Feb 3. PubMed PMID: 33533239; PubMed Central PMCID: PMC9847585.

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Singh A, Turner JM, Tomberg J, Fedarovich A, Unemo M, Nicholas RA, Davies C. Mutations in penicillin-binding protein 2 from cephalosporin-resistant Neisseria gonorrhoeae hinder ceftriaxone acylation by restricting protein dynamics. J Biol Chem. 2020 May 22;295(21):7529-7543. doi: 10.1074/jbc.RA120.012617. Epub 2020 Apr 6. PubMed PMID: 32253235; PubMed Central PMCID: PMC7247294.

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Singh A, Tomberg J, Nicholas RA, Davies C. Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2. J Biol Chem. 2019 Sep 20;294(38):14020-14032. doi: 10.1074/jbc.RA119.009942. Epub 2019 Jul 30. PubMed PMID: 31362987; PubMed Central PMCID: PMC6755799.

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Cristillo AD, Bristow CC, Torrone E, Dillon JA, Kirkcaldy RD, Dong H, Grad YH, Nicholas RA, Rice PA, Lawrence K, Oldach D, Shafer WM, Zhou P, Wi TE, Morris SR, Klausner JD. Antimicrobial Resistance in Neisseria gonorrhoeae: Proceedings of the STAR Sexually Transmitted Infection-Clinical Trial Group Programmatic Meeting. Sex Transm Dis. 2019 Mar;46(3):e18-e25. doi: 10.1097/OLQ.0000000000000929. PubMed PMID: 30363025; PubMed Central PMCID: PMC6370498.

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Baarda BI, Zielke RA, Nicholas RA, Sikora AE. PubMLST for Antigen Allele Mining to Inform Development of Gonorrhea Protein-Based Vaccines. Front Microbiol. 2018;9:2971. doi: 10.3389/fmicb.2018.02971. eCollection 2018. PubMed PMID: 30581422; PubMed Central PMCID: PMC6292995.

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Zhu W, Tomberg J, Knilans KJ, Anderson JE, McKinnon KP, Sempowski GD, Nicholas RA, Duncan JA. Properly folded and functional PorB from Neisseria gonorrhoeae inhibits dendritic cell stimulation of CD4(+) T cell proliferation. J Biol Chem. 2018 Jul 13;293(28):11218-11229. doi: 10.1074/jbc.RA117.001209. Epub 2018 May 11. PubMed PMID: 29752412; PubMed Central PMCID: PMC6052219.

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Vincent LR, Kerr SR, Tan Y, Tomberg J, Raterman EL, Dunning Hotopp JC, Unemo M, Nicholas RA, Jerse AE. In Vivo-Selected Compensatory Mutations Restore the Fitness Cost of Mosaic penA Alleles That Confer Ceftriaxone Resistance in Neisseria gonorrhoeae. mBio. 2018 Apr 3;9(2). doi: 10.1128/mBio.01905-17. PubMed PMID: 29615507; PubMed Central PMCID: PMC5885032.

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Rouquette-Loughlin CE, Zalucki YM, Dhulipala VL, Balthazar JT, Doyle RG, Nicholas RA, Begum AA, Raterman EL, Jerse AE, Shafer WM. Control of gdhR Expression in Neisseria gonorrhoeae via Autoregulation and a Master Repressor (MtrR) of a Drug Efflux Pump Operon. mBio. 2017 Apr 11;8(2). doi: 10.1128/mBio.00449-17. PubMed PMID: 28400529; PubMed Central PMCID: PMC5388806.

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Tomberg J, Fedarovich A, Vincent LR, Jerse AE, Unemo M, Davies C, Nicholas RA. Alanine 501 Mutations in Penicillin-Binding Protein 2 from Neisseria gonorrhoeae: Structure, Mechanism, and Effects on Cephalosporin Resistance and Biological Fitness. Biochemistry. 2017 Feb 28;56(8):1140-1150. doi: 10.1021/acs.biochem.6b01030. Epub 2017 Feb 16. PubMed PMID: 28145684; PubMed Central PMCID: PMC5502787.

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Nemmara VV, Nicholas RA, Pratt RF. Synthesis and Kinetic Analysis of Two Conformationally Restricted Peptide Substrates of Escherichia coli Penicillin-Binding Protein 5. Biochemistry. 2016 Jul 26;55(29):4065-76. doi: 10.1021/acs.biochem.6b00576. Epub 2016 Jul 15. PubMed PMID: 27420403.

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Abrams AJ, Trees DL, Nicholas RA. Complete Genome Sequences of Three Neisseria gonorrhoeae Laboratory Reference Strains, Determined Using PacBio Single-Molecule Real-Time Technology. Genome Announc. 2015 Sep 10;3(5). doi: 10.1128/genomeA.01052-15. PubMed PMID: 26358608; PubMed Central PMCID: PMC4566190.

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Nandi S, Swanson S, Tomberg J, Nicholas RA. Diffusion of antibiotics through the PilQ secretin in Neisseria gonorrhoeae occurs through the immature, sodium dodecyl sulfate-labile form. J Bacteriol. 2015 Apr;197(8):1308-21. doi: 10.1128/JB.02628-14. Epub 2015 Jan 20. PubMed PMID: 25605303; PubMed Central PMCID: PMC4372736.

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Nicholas RA. Insights into platelet P2Y12 receptor activation. Blood. 2015 Feb 5;125(6):893-5. doi: 10.1182/blood-2014-12-615336. PubMed PMID: 25655454.

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Fedarovich A, Cook E, Tomberg J, Nicholas RA, Davies C. Structural effect of the Asp345a insertion in penicillin-binding protein 2 from penicillin-resistant strains of Neisseria gonorrhoeae. Biochemistry. 2014 Dec 9;53(48):7596-603. doi: 10.1021/bi5011317. Epub 2014 Dec 1. PubMed PMID: 25403720; PubMed Central PMCID: PMC4263433.

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Morrow GB, Nicholas RA, Kennedy C. UTP is not a biased agonist at human P2Y(11) receptors. Purinergic Signal. 2014 Dec;10(4):581-5. doi: 10.1007/s11302-014-9418-3. PubMed PMID: 25015314; PubMed Central PMCID: PMC4272372.

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Qi AD, Harden TK, Nicholas RA. Is GPR17 a P2Y/leukotriene receptor? examination of uracil nucleotides, nucleotide sugars, and cysteinyl leukotrienes as agonists of GPR17. J Pharmacol Exp Ther. 2013 Oct;347(1):38-46. doi: 10.1124/jpet.113.207647. Epub 2013 Aug 1. PubMed PMID: 23908386; PubMed Central PMCID: PMC3781415.

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Tomberg J, Unemo M, Ohnishi M, Davies C, Nicholas RA. Identification of amino acids conferring high-level resistance to expanded-spectrum cephalosporins in the penA gene from Neisseria gonorrhoeae strain H041. Antimicrob Agents Chemother. 2013 Jul;57(7):3029-36. doi: 10.1128/AAC.00093-13. Epub 2013 Apr 15. PubMed PMID: 23587946; PubMed Central PMCID: PMC3697319.

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DuBose DR, Wolff SC, Qi AD, Naruszewicz I, Nicholas RA. Apical targeting of the P2Y(4) receptor is directed by hydrophobic and basic residues in the cytoplasmic tail. Am J Physiol Cell Physiol. 2013 Feb 1;304(3):C228-39. doi: 10.1152/ajpcell.00251.2012. Epub 2012 Oct 10. PubMed PMID: 23054062; PubMed Central PMCID: PMC3566436.

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Unemo M, Nicholas RA. Emergence of multidrug-resistant, extensively drug-resistant and untreatable gonorrhea. Future Microbiol. 2012 Dec;7(12):1401-22. doi: 10.2217/fmb.12.117. Review. PubMed PMID: 23231489; PubMed Central PMCID: PMC3629839.

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Fedarovich A, Djordjevic KA, Swanson SM, Peterson YK, Nicholas RA, Davies C. High-throughput screening for novel inhibitors of Neisseria gonorrhoeae penicillin-binding protein 2. PLoS One. 2012;7(9):e44918. doi: 10.1371/journal.pone.0044918. Epub 2012 Sep 25. PubMed PMID: 23049763; PubMed Central PMCID: PMC3458020.

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Fedarovich A, Nicholas RA, Davies C. The role of the β5-α11 loop in the active-site dynamics of acylated penicillin-binding protein A from Mycobacterium tuberculosis. J Mol Biol. 2012 May 18;418(5):316-30. doi: 10.1016/j.jmb.2012.02.021. Epub 2012 Feb 23. PubMed PMID: 22365933; PubMed Central PMCID: PMC3327805.

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Tomberg J, Temple B, Fedarovich A, Davies C, Nicholas RA. A highly conserved interaction involving the middle residue of the SXN active-site motif is crucial for function of class B penicillin-binding proteins: mutational and computational analysis of PBP 2 from N. gonorrhoeae. Biochemistry. 2012 Apr 3;51(13):2775-84. doi: 10.1021/bi2017987. Epub 2012 Mar 22. PubMed PMID: 22397678; PubMed Central PMCID: PMC3338128.

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Unemo M, Golparian D, Nicholas R, Ohnishi M, Gallay A, Sednaoui P. High-level cefixime- and ceftriaxone-resistant Neisseria gonorrhoeae in France: novel penA mosaic allele in a successful international clone causes treatment failure. Antimicrob Agents Chemother. 2012 Mar;56(3):1273-80. doi: 10.1128/AAC.05760-11. Epub 2011 Dec 12. PubMed PMID: 22155830; PubMed Central PMCID: PMC3294892.

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Nemmara VV, Dzhekieva L, Sarkar KS, Adediran SA, Duez C, Nicholas RA, Pratt RF. Substrate specificity of low-molecular mass bacterial DD-peptidases. Biochemistry. 2011 Nov 22;50(46):10091-101. doi: 10.1021/bi201326a. Epub 2011 Oct 27. PubMed PMID: 22029692.

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El-Tayeb A, Qi A, Nicholas RA, Müller CE. Structural modifications of UMP, UDP, and UTP leading to subtype-selective agonists for P2Y2, P2Y4, and P2Y6 receptors. J Med Chem. 2011 Apr 28;54(8):2878-90. doi: 10.1021/jm1016297. Epub 2011 Mar 21. PubMed PMID: 21417463.

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Qi AD, Houston-Cohen D, Naruszewicz I, Harden TK, Nicholas RA. Ser352 and Ser354 in the carboxyl terminus of the human P2Y(1) receptor are required for agonist-promoted phosphorylation and internalization in MDCK cells. Br J Pharmacol. 2011 Mar;162(6):1304-13. doi: 10.1111/j.1476-5381.2010.01135.x. PubMed PMID: 21108629; PubMed Central PMCID: PMC3058163.

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Lee CJ, Liang X, Chen X, Zeng D, Joo SH, Chung HS, Barb AW, Swanson SM, Nicholas RA, Li Y, Toone EJ, Raetz CR, Zhou P. Species-specific and inhibitor-dependent conformations of LpxC: implications for antibiotic design. Chem Biol. 2011 Jan 28;18(1):38-47. doi: 10.1016/j.chembiol.2010.11.011. Epub 2010 Dec 16. PubMed PMID: 21167751; PubMed Central PMCID: PMC3149848.

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Nicola G, Tomberg J, Pratt RF, Nicholas RA, Davies C. Crystal structures of covalent complexes of β-lactam antibiotics with Escherichia coli penicillin-binding protein 5: toward an understanding of antibiotic specificity. Biochemistry. 2010 Sep 21;49(37):8094-104. doi: 10.1021/bi100879m. PubMed PMID: 20726582; PubMed Central PMCID: PMC2947372.

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Tomberg J, Unemo M, Davies C, Nicholas RA. Molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in Neisseria gonorrhoeae: role of epistatic mutations. Biochemistry. 2010 Sep 21;49(37):8062-70. doi: 10.1021/bi101167x. PubMed PMID: 20704258; PubMed Central PMCID: PMC2939205.

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Wolff SC, Qi AD, Harden TK, Nicholas RA. Charged residues in the C-terminus of the P2Y1 receptor constitute a basolateral-sorting signal. J Cell Sci. 2010 Jul 15;123(Pt 14):2512-20. doi: 10.1242/jcs.060723. PubMed PMID: 20592187; PubMed Central PMCID: PMC2894661.

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Fedarovich A, Nicholas RA, Davies C. Unusual conformation of the SxN motif in the crystal structure of penicillin-binding protein A from Mycobacterium tuberculosis. J Mol Biol. 2010 Apr 23;398(1):54-65. doi: 10.1016/j.jmb.2010.02.046. Epub 2010 Mar 3. PubMed PMID: 20206184; PubMed Central PMCID: PMC2854034.

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Zhao S, Duncan M, Tomberg J, Davies C, Unemo M, Nicholas RA. Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae. Antimicrob Agents Chemother. 2009 Sep;53(9):3744-51. doi: 10.1128/AAC.00304-09. Epub 2009 Jun 15. PubMed PMID: 19528266; PubMed Central PMCID: PMC2737842.

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Peddi S, Nicholas RA, Gutheil WG. Neisseria gonorrhoeae penicillin-binding protein 3 demonstrates a pronounced preference for N(epsilon)-acylated substrates. Biochemistry. 2009 Jun 23;48(24):5731-7. doi: 10.1021/bi9003099. PubMed PMID: 19413336; PubMed Central PMCID: PMC2756183.

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Hillmann P, Ko GY, Spinrath A, Raulf A, von Kügelgen I, Wolff SC, Nicholas RA, Kostenis E, Höltje HD, Müller CE. Key determinants of nucleotide-activated G protein-coupled P2Y(2) receptor function revealed by chemical and pharmacological experiments, mutagenesis and homology modeling. J Med Chem. 2009 May 14;52(9):2762-75. doi: 10.1021/jm801442p. PubMed PMID: 19419204.

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Sesma JI, Esther CR Jr, Kreda SM, Jones L, O'Neal W, Nishihara S, Nicholas RA, Lazarowski ER. Endoplasmic reticulum/golgi nucleotide sugar transporters contribute to the cellular release of UDP-sugar signaling molecules. J Biol Chem. 2009 May 1;284(18):12572-83. doi: 10.1074/jbc.M806759200. Epub 2009 Mar 10. PubMed PMID: 19276090; PubMed Central PMCID: PMC2673323.

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Powell AJ, Tomberg J, Deacon AM, Nicholas RA, Davies C. Crystal structures of penicillin-binding protein 2 from penicillin-susceptible and -resistant strains of Neisseria gonorrhoeae reveal an unexpectedly subtle mechanism for antibiotic resistance. J Biol Chem. 2009 Jan 9;284(2):1202-12. doi: 10.1074/jbc.M805761200. Epub 2008 Nov 4. PubMed PMID: 18986991; PubMed Central PMCID: PMC2613624.

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Magnone M, Basile G, Bruzzese D, Guida L, Signorello MG, Chothi MP, Bruzzone S, Millo E, Qi AD, Nicholas RA, Kassack MU, Leoncini G, Zocchi E. Adenylic dinucleotides produced by CD38 are negative endogenous modulators of platelet aggregation. J Biol Chem. 2008 Sep 5;283(36):24460-8. doi: 10.1074/jbc.M710568200. Epub 2008 Jul 7. PubMed PMID: 18606819; PubMed Central PMCID: PMC3259817.

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Vernel-Pauillac F, Nandi S, Nicholas RA, Goarant C. Genotyping as a tool for antibiotic resistance surveillance of Neisseria gonorrhoeae in New Caledonia: evidence of a novel genotype associated with reduced penicillin susceptibility. Antimicrob Agents Chemother. 2008 Sep;52(9):3293-300. doi: 10.1128/AAC.00020-08. Epub 2008 Jun 30. PubMed PMID: 18591264; PubMed Central PMCID: PMC2533457.

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Fricks IP, Maddileti S, Carter RL, Lazarowski ER, Nicholas RA, Jacobson KA, Harden TK. UDP is a competitive antagonist at the human P2Y14 receptor. J Pharmacol Exp Ther. 2008 May;325(2):588-94. doi: 10.1124/jpet.108.136309. Epub 2008 Feb 5. PubMed PMID: 18252808; PubMed Central PMCID: PMC2918431.

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Moreschi I, Bruzzone S, Bodrato N, Usai C, Guida L, Nicholas RA, Kassack MU, Zocchi E, De Flora A. NAADP+ is an agonist of the human P2Y11 purinergic receptor. Cell Calcium. 2008 Apr;43(4):344-55. doi: 10.1016/j.ceca.2007.06.006. Epub 2007 Aug 17. PubMed PMID: 17707504.

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Folster JP, Dhulipala V, Nicholas RA, Shafer WM. Differential regulation of ponA and pilMNOPQ expression by the MtrR transcriptional regulatory protein in Neisseria gonorrhoeae. J Bacteriol. 2007 Jul;189(13):4569-77. doi: 10.1128/JB.00286-07. Epub 2007 May 4. PubMed PMID: 17483228; PubMed Central PMCID: PMC1913451.

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Lindberg R, Fredlund H, Nicholas R, Unemo M. Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponA. Antimicrob Agents Chemother. 2007 Jun;51(6):2117-22. doi: 10.1128/AAC.01604-06. Epub 2007 Apr 9. PubMed PMID: 17420216; PubMed Central PMCID: PMC1891421.

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Josephine HR, Charlier P, Davies C, Nicholas RA, Pratt RF. Reactivity of penicillin-binding proteins with peptidoglycan-mimetic beta-lactams: what's wrong with these enzymes?. Biochemistry. 2006 Dec 26;45(51):15873-83. doi: 10.1021/bi061804f. Epub 2006 Dec 1. PubMed PMID: 17176110.

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Moreschi I, Bruzzone S, Nicholas RA, Fruscione F, Sturla L, Benvenuto F, Usai C, Meis S, Kassack MU, Zocchi E, De Flora A. Extracellular NAD+ is an agonist of the human P2Y11 purinergic receptor in human granulocytes. J Biol Chem. 2006 Oct 20;281(42):31419-29. doi: 10.1074/jbc.M606625200. Epub 2006 Aug 22. PubMed PMID: 16926152.

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Fedarovich A, Tomberg J, Nicholas RA, Davies C. Structure of the N-terminal domain of human CEACAM1: binding target of the opacity proteins during invasion of Neisseria meningitidis and N. gonorrhoeae. Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):971-9. doi: 10.1107/S0907444906020737. Epub 2006 Aug 19. PubMed PMID: 16929097.

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Okada SF, Nicholas RA, Kreda SM, Lazarowski ER, Boucher RC. Physiological regulation of ATP release at the apical surface of human airway epithelia. J Biol Chem. 2006 Aug 11;281(32):22992-3002. doi: 10.1074/jbc.M603019200. Epub 2006 Jun 5. PubMed PMID: 16754672; PubMed Central PMCID: PMC2924190.

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Powell AJ, Liu ZJ, Nicholas RA, Davies C. Crystal structures of the lytic transglycosylase MltA from N.gonorrhoeae and E.coli: insights into interdomain movements and substrate binding. J Mol Biol. 2006 May 26;359(1):122-36. doi: 10.1016/j.jmb.2006.03.023. Epub 2006 Mar 29. PubMed PMID: 16618494.

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Olesky M, Zhao S, Rosenberg RL, Nicholas RA. Porin-mediated antibiotic resistance in Neisseria gonorrhoeae: ion, solute, and antibiotic permeation through PIB proteins with penB mutations. J Bacteriol. 2006 Apr;188(7):2300-8. doi: 10.1128/JB.188.7.2300-2308.2006. PubMed PMID: 16547016; PubMed Central PMCID: PMC1428387.