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Year Number of Results
1983 8
1984 11
1985 31
1986 41
1987 69
1988 88
1989 104
1990 116
1991 111
1992 130
1993 132
1994 111
1995 95
1996 105
1997 70
1998 72
1999 49
2000 46
2001 41
2002 25
2003 33
2004 18
2005 17
2006 21
2007 16
2008 20
2009 13
2010 16
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2013 13
2014 12
2015 11
2016 3
2017 8
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2024 1

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1,648 results

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Page 1
New purification method for glucocorticoid receptors.
Okamoto K, Isohashi F. Okamoto K, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 25;797(1-2):367-71. doi: 10.1016/s1570-0232(03)00429-x. J Chromatogr B Analyt Technol Biomed Life Sci. 2003. PMID: 14630162 Review.
This method is based upon a new finding that activated GR binds to both Mono Q and Mono S columns at the same pH. This method enables us to purify recombinant GR within 3 h. ...
This method is based upon a new finding that activated GR binds to both Mono Q and Mono S columns at the same pH. This method …
Trypsin-chymotrypsin inhibitors from Vigna mungo seeds.
Cheung AH, Wong JH, Ng TB. Cheung AH, et al. Protein Pept Lett. 2009;16(3):277-84. doi: 10.2174/092986609787601714. Protein Pept Lett. 2009. PMID: 19275741
Three trypsin-chymotrypsin inhibitors were isolated from seeds of the black gram (Vigna mungo) with a procedure that entailed cation exchange chromatography on SP-Sepharose, anion exchange chromatography on Q-Sepharose, ion exchange chromatography by fast protein liquid chromatog …
Three trypsin-chymotrypsin inhibitors were isolated from seeds of the black gram (Vigna mungo) with a procedure that entailed cation exchang …
Hepatic production of 1,5-anhydrofructose and 1,5-anhydroglucitol in rat by the third glycogenolytic pathway.
Kametani S, Shiga Y, Akanuma H. Kametani S, et al. Eur J Biochem. 1996 Dec 15;242(3):832-8. doi: 10.1111/j.1432-1033.1996.0832r.x. Eur J Biochem. 1996. PMID: 9022716 Free article.
We also demonstrated that 1,5AnFru is produced in the cytosol fraction of rat liver homogenate when an alpha-1,4-glucan or glycogen was added; 1,5AnFru was readily reduced to 1,5-anhydroglucitol with NADPH or at a reduced efficiency with NADH in the presence of a Mono Q …
We also demonstrated that 1,5AnFru is produced in the cytosol fraction of rat liver homogenate when an alpha-1,4-glucan or glycogen was adde …
Flavanone-7-O-glucosyltransferase activity from Petunia hybrida.
Durren RL, McIntosh CA. Durren RL, et al. Phytochemistry. 1999 Nov;52(5):793-8. doi: 10.1016/s0031-9422(99)00307-6. Phytochemistry. 1999. PMID: 10626374
This activity was partially purified 89-fold via ammonium sulfate fractionation followed by FPLC on Superose 12 and Mono Q yielding three chromatographically separate peaks of activity. The enzymes in the peak fractions glucosylated flavanone, flavonol, and flavone …
This activity was partially purified 89-fold via ammonium sulfate fractionation followed by FPLC on Superose 12 and Mono Q yie …
Purification of epidermal plasminogen activator inhibitor.
Hibino T, Izaki S, Izaki M. Hibino T, et al. FEBS Lett. 1986 Nov 24;208(2):273-7. doi: 10.1016/0014-5793(86)81031-6. FEBS Lett. 1986. PMID: 3096778 Free article.
A plasminogen activator inhibitor was purified from human cornified cell extract by DEAE-Sepharose, Sephacryl S-200, and high-performance liquid chromatographies on hydroxyapatite HPHT and anion-exchanger Mono Q at pH 7.2 and 8.0. The purified inhibitor showed Mr 43 …
A plasminogen activator inhibitor was purified from human cornified cell extract by DEAE-Sepharose, Sephacryl S-200, and high-performance li …
Structural analysis of a human glial variant laminin.
LeMosy EK, Lightner VA, Erickson HP. LeMosy EK, et al. Exp Cell Res. 1996 Aug 25;227(1):80-8. doi: 10.1006/excr.1996.0252. Exp Cell Res. 1996. PMID: 8806454 Free article.
We previously reported the presence of an astrocyte-derived variant laminin in media conditioned by human U251 MG astrocytoma cells. This laminin was partially purified in a highly anionic Mono Q fraction with strong adhesion activity for fibroblasts and glial cells …
We previously reported the presence of an astrocyte-derived variant laminin in media conditioned by human U251 MG astrocytoma cells. This la …
Separation of assembly-competent tubulin from brain microtubule protein preparations using a fast-performance liquid chromatography procedure.
Roychowdhury S, Gaskin F. Roychowdhury S, et al. J Neurochem. 1986 May;46(5):1399-405. doi: 10.1111/j.1471-4159.1986.tb01754.x. J Neurochem. 1986. PMID: 3007670
The Mono Q-purified tubulin fraction showed trace high-molecular-weight components, which were removed on Mono S (cation exchanger) columns. ...Tubulin, trace nontubulin proteins, and several enzymatic activities came off in the void volume. A combination of Mono …
The Mono Q-purified tubulin fraction showed trace high-molecular-weight components, which were removed on Mono S (cation excha …
Purification and characterization of glycosyl-phosphatidylinositol-specific phospholipase D.
Huang KS, Li S, Fung WJ, Hulmes JD, Reik L, Pan YC, Low MG. Huang KS, et al. J Biol Chem. 1990 Oct 15;265(29):17738-45. J Biol Chem. 1990. PMID: 2170394 Free article.
The enzyme was initially purified by a procedure consisting of 9% polyethylene glycol precipitation, Q Sepharose anion-exchange chromatography, S-300 gel filtration, wheat germ lectin-Sepharose, hydroxylapatite agarose, zinc chelate matrix, Mono Q-high performance l …
The enzyme was initially purified by a procedure consisting of 9% polyethylene glycol precipitation, Q Sepharose anion-exchange chromatograp …
A cytokine-inducing hemagglutinin from small taros.
Chan YS, Wong JH, Ng TB. Chan YS, et al. Protein Pept Lett. 2010 Jul;17(7):823-30. doi: 10.2174/092986610791306742. Protein Pept Lett. 2010. PMID: 19807671
'Small Taro' by employing a purification protocol that involved ion exchange chromatography on Q-Sepharose, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono Q, and FPLC-gel filtration on Superdex 75. The hemagglutinin was isolated from t …
'Small Taro' by employing a purification protocol that involved ion exchange chromatography on Q-Sepharose, fast protein liquid chromatograp …
Interleukin-1 activates a novel protein kinase that phosphorylates the epidermal-growth-factor receptor peptide T669.
Kracht M, Shiroo M, Marshall CJ, Hsuan JJ, Saklatvala J. Kracht M, et al. Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):897-905. doi: 10.1042/bj3020897. Biochem J. 1994. PMID: 7945218 Free PMC article.
The enzyme, which had an apparent molecular mass of 45 kDa on gel-filtration chromatography, was purified 170,000-fold from cytosolic extracts by sequential chromatography on Mono Q, Mono S, phenyl-Sepharose, Superose 12, ATP-Sepharose and Mono Q. ...
The enzyme, which had an apparent molecular mass of 45 kDa on gel-filtration chromatography, was purified 170,000-fold from cytosolic extrac …
1,648 results