Isolation and characterization of a 36-kDa microfibril-associated glycoprotein by the newly synthesized isoquinolinesulfonamide affinity chromatography

Biochem Biophys Res Commun. 1994 Feb 15;198(3):1262-6. doi: 10.1006/bbrc.1994.1178.

Abstract

We have reported here the biochemical characterization of a newly identified microfibril-associated protein of 36-kDa (36-kDa MAP) from bovine aorta. Using Ca(2+)-dependent affinity chromatography on an isoquinolinesulfonamide derivative (CKA-1303)-coupled Sepharose, we obtained a pure form of 36-kDa MAP. This compound should serve as a useful tool for clarifying the physiological roles of 36-kDa MAP. In addition, 45Ca-autoradiography clearly indicated that 36-kDa MAP binds Ca2+. Partial amino acid sequence of 36-kDa MAP was determined. Our search of that published sequence against a 36-kDa MAP sequence revealed the resemblance to fibrinogen-related proteins of sea cucumber, cytotactin and tenascin which are substrate-adhesion proteins made at critical stages of histogenesis.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aorta / metabolism
  • Calcium / metabolism
  • Calcium-Binding Proteins / chemistry
  • Calcium-Binding Proteins / isolation & purification*
  • Calcium-Binding Proteins / metabolism
  • Cattle
  • Chromatography, Affinity / methods*
  • Cinnamates / chemical synthesis
  • Contractile Proteins*
  • Extracellular Matrix Proteins*
  • Glycoproteins / chemistry
  • Glycoproteins / isolation & purification*
  • Glycoproteins / metabolism
  • Humans
  • Indicators and Reagents
  • Isoquinolines / chemical synthesis
  • Molecular Sequence Data
  • Molecular Weight
  • Muscle, Smooth, Vascular / metabolism
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification
  • RNA Splicing Factors
  • Sequence Homology, Amino Acid

Substances

  • Calcium-Binding Proteins
  • Cinnamates
  • Contractile Proteins
  • Extracellular Matrix Proteins
  • Glycoproteins
  • Indicators and Reagents
  • Isoquinolines
  • Peptide Fragments
  • RNA Splicing Factors
  • microfibrillar protein
  • CKA 1303
  • Calcium