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Page 1
Incorporation of fluorotryptophans into proteins of escherichia coli.
Pratt EA, Ho C. Pratt EA, et al. Biochemistry. 1975 Jul;14(13):3035-40. doi: 10.1021/bi00684a037. Biochemistry. 1975. PMID: 1096937
When beta-galactosidase is induced at the time of addition of analog, it reaches 60% of the control specific activity in the presence of 4-fluorotryptophan, 10% of normal in the presence of 5- or 6-fluorotryptophan. Lactose permease activity is 35% of the control in …
When beta-galactosidase is induced at the time of addition of analog, it reaches 60% of the control specific activity in the presence of 4-f …
Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR.
Baugher AH, Goetz JM, McDowell LM, Huang H, Wooley KL, Schaefer J. Baugher AH, et al. Biophys J. 1998 Nov;75(5):2574-6. doi: 10.1016/S0006-3495(98)77702-1. Biophys J. 1998. PMID: 9788953 Free PMC article.
Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. …
Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complex …
Determination of serotonin turnover in the rat brain using 6-fluorotryptophan.
Miwa S, Fujiwara M, Lee K, Fujiwara M. Miwa S, et al. J Neurochem. 1987 May;48(5):1577-80. doi: 10.1111/j.1471-4159.1987.tb05704.x. J Neurochem. 1987. PMID: 2435850
After intraperitoneal injection of rats with 6-fluorotryptophan (6-FT), brain 5-hydroxytryptamine (5-HT) levels decreased exponentially over 1 h. ...
After intraperitoneal injection of rats with 6-fluorotryptophan (6-FT), brain 5-hydroxytryptamine (5-HT) levels decreased expo …
Ex Vivo Analysis of Tryptophan Metabolism Using 19F NMR.
Tombari RJ, Saunders CM, Wu CY, Dunlap LE, Tantillo DJ, Olson DE. Tombari RJ, et al. ACS Chem Biol. 2019 Sep 20;14(9):1866-1873. doi: 10.1021/acschembio.9b00548. Epub 2019 Aug 26. ACS Chem Biol. 2019. PMID: 31449387 Free PMC article.
There are three principal routes by which tryptophan is degraded, and thus methods for measuring metabolic flux through these pathways can be used to understand the factors that perturb tryptophan metabolism and potentially to measure disease biomarkers. Here, we describe a metho …
There are three principal routes by which tryptophan is degraded, and thus methods for measuring metabolic flux through these pathways can b …
19F NMR studies of tryptophan/serum albumin binding.
Cao B, Endsley S, Andersen NH. Cao B, et al. Bioorg Med Chem. 2003 Jan 2;11(1):69-75. doi: 10.1016/s0968-0896(02)00324-3. Bioorg Med Chem. 2003. PMID: 12467709
19F NMR provides direct measures of the Trp binding avidity of 'fatty acid free' bovine serum albumin when D- and L-6-fluorotryptophan are used as the probes. Both a high and low affinity binding site are present. ...Binding at this site appears to be hydrophobic an …
19F NMR provides direct measures of the Trp binding avidity of 'fatty acid free' bovine serum albumin when D- and L-6-fluorotrypto
2-Fluorotyrosine is a valuable but understudied amino acid for protein-observed 19F NMR.
Ycas PD, Wagner N, Olsen NM, Fu R, Pomerantz WCK. Ycas PD, et al. J Biomol NMR. 2020 Jan;74(1):61-69. doi: 10.1007/s10858-019-00290-0. Epub 2019 Nov 23. J Biomol NMR. 2020. PMID: 31760571
The study of (19)F labeled aromatic amino acids has largely focused on 4-,5-, or 6-fluorotryptophan, 4-fluorophenylalanine, (4,5, or 6FW) or 3-fluorotyrosine (3FY), whereas 2-fluorotyrosine (2FY) has remained largely understudied. ...
The study of (19)F labeled aromatic amino acids has largely focused on 4-,5-, or 6-fluorotryptophan, 4-fluorophenylalanine, (4 …
Nuclear magnetic resonance studies of 6-fluorotryptophan-substituted rat cellular retinol-binding protein II produced in Escherichia coli. Analysis of the apoprotein and the holoprotein containing bound all-trans-retinol and all-trans-retinal.
Li E, Quian SJ, Nader L, Yang NC, d'Avignon A, Sacchettini JC, Gordon JI. Li E, et al. J Biol Chem. 1989 Oct 15;264(29):17041-8. J Biol Chem. 1989. PMID: 2676998 Free article.
To further investigate ligand-protein interactions, we have utilized 19F nuclear magnetic resonance (NMR) spectroscopy of CRBP II labeled at its 4 tryptophan residues with 6-fluorotryptophan. Efficient incorporation of 6-fluorotryptophan (93%) was achi …
To further investigate ligand-protein interactions, we have utilized 19F nuclear magnetic resonance (NMR) spectroscopy of CRBP II labeled at …
Precursor-Directed Biosynthesis of Antialgal Fluorinated Bacillamide Derivatives in Bacillus atrophaeus.
Saalim M, Liu S, Bennett SD, Zaleta-Pinet DA, Poulin RX, Clark BR. Saalim M, et al. J Nat Prod. 2024 Feb 23;87(2):388-395. doi: 10.1021/acs.jnatprod.3c01178. Epub 2024 Feb 6. J Nat Prod. 2024. PMID: 38319739
Incorporation of fluorine into the bacillamides was carried out using a precursor-directed biosynthesis approach, with 4-, 5-, and 6-fluorotryptophan added to growing cultures of Bacillus atrophaeus IMG-11. ...
Incorporation of fluorine into the bacillamides was carried out using a precursor-directed biosynthesis approach, with 4-, 5-, and 6- …
77 results