Preparation and antigenic property of 3-dehydrolithocholylglycine 3-(O-carboxymethyl) oxime-bovine serum albumin conjugate

Steroids. 1983 Feb;41(2):165-72. doi: 10.1016/0039-128x(83)90005-3.

Abstract

The preparation and antigenic property of 3-dehydrolithocholyglycine-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-carboxymethyl) oxime bridge at the C-3 position on the steroid nucleus is described. Antibody raised against antigen in the rabbit possessed high titer and specificity to lithocholylglycine, exhibiting no significant cross-reaction with free lithocholic acid or lithocholyltaurine.

MeSH terms

  • Antigen-Antibody Complex
  • Epitopes / analysis
  • Haptens
  • Immune Sera
  • Indicators and Reagents
  • Lithocholic Acid / analogs & derivatives*
  • Lithocholic Acid / chemical synthesis
  • Magnetic Resonance Spectroscopy
  • Serum Albumin, Bovine
  • Spectrophotometry, Infrared

Substances

  • Antigen-Antibody Complex
  • Epitopes
  • Haptens
  • Immune Sera
  • Indicators and Reagents
  • Serum Albumin, Bovine
  • Lithocholic Acid
  • 3-dehydrolithocholylglycine