12-O-acetylphorbol-13-decanoate potently inhibits cytopathic effects of human immunodeficiency virus type 1 (HIV-1), without activation of protein kinase C

S el-Mekkawy; M R Meselhy; N Nakamura; M Hattori; T Kawahata; T Otake

doi:10.1248/cpb.47.1346

12-O-acetylphorbol-13-decanoate potently inhibits cytopathic effects of human immunodeficiency virus type 1 (HIV-1), without activation of protein kinase C

Chem Pharm Bull (Tokyo). 1999 Sep;47(9):1346-7. doi: 10.1248/cpb.47.1346.

Authors

S el-Mekkawy¹, M R Meselhy, N Nakamura, M Hattori, T Kawahata, T Otake

Affiliation

¹ Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Japan.

PMID: 10517016
DOI: 10.1248/cpb.47.1346

Abstract

Through bioactivity-guided fractionation, eight phorbol diesters, including five new ones (1-5), were isolated from the seeds of Croton tiglium collected in Egypt. 12-O-Acetylphorbol-13-decanoate (6) and 12-O-decanoylphorbol-13-(2-methylbutyrate) (4) potently inhibited the HIV-1-induced cytopathic effect on MT-4 cells (IC100 values of 7.6 ng/ml and 7.81 micrograms/ml, and CC0 values of 62.5 micrograms/ml and 31.3 micrograms/ml, respectively) without activating protein kinase C.

MeSH terms

Anti-HIV Agents / chemical synthesis*
Anti-HIV Agents / pharmacology
Cytopathogenic Effect, Viral / drug effects
Decanoates / chemical synthesis*
Decanoates / pharmacology
Egypt
Enzyme Activation / drug effects
HIV-1 / drug effects*
HIV-1 / enzymology
Humans
Phorbols / chemical synthesis*
Phorbols / pharmacology
Plants, Medicinal / chemistry
Protein Kinase C / metabolism*
Seeds / chemistry

Substances

Anti-HIV Agents
Decanoates
Phorbols
12-O-acetylphorbol-13-decanoate
Protein Kinase C