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Combining Metabolic Engineering and Lipid Droplet Storage Engineering for Improved alpha-Bisabolene Production in Yarrowia Lipolytica.
Lu Z, Wang Y, Li Z, Zhang Y, He S, Zhang Z, Leong S, Wong A, Zhang CY, Yu A. Lu Z, et al. J Agric Food Chem. 2023 Aug 2;71(30):11534-11543. doi: 10.1021/acs.jafc.3c02472. Epub 2023 Jul 18. J Agric Food Chem. 2023. PMID: 37463315
Microbial production offers a green, efficient, and sustainable alternative. In this study, we focused on improving the titers of alpha-bisabolene in Yarrowia lipolytica by applying two strategies, (i) optimizing the metabolic flux of alpha-bisabolene …
Microbial production offers a green, efficient, and sustainable alternative. In this study, we focused on improving the titers of alpha …
High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.
Zhao Y, Zhu K, Li J, Zhao Y, Li S, Zhang C, Xiao D, Yu A. Zhao Y, et al. Microb Biotechnol. 2021 Nov;14(6):2497-2513. doi: 10.1111/1751-7915.13768. Epub 2021 Feb 19. Microb Biotechnol. 2021. PMID: 33605546 Free PMC article.
In this proof-of-principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce alpha-bisabolene, beta-bisabolene and gamma-bisabolene through heterologous expression of the alpha-bisabolene synthase from Abies g …
In this proof-of-principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce alpha-bisa …
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-alpha-bisabolene.
Ichinose H, Ukeba S, Kitaoka T. Ichinose H, et al. Enzyme Microb Technol. 2022 Aug;158:110037. doi: 10.1016/j.enzmictec.2022.110037. Epub 2022 Mar 29. Enzyme Microb Technol. 2022. PMID: 35378352
Metabolic studies revealed that seven of the PcSTSs produce a series of sesquiterpene scaffolds, including (E)-alpha-bisabolene. Furthermore, we constructed a co-expression system of (E)-alpha-bisabolene synthase and P450 from P. chrysosporium (PcCYP). …
Metabolic studies revealed that seven of the PcSTSs produce a series of sesquiterpene scaffolds, including (E)-alpha-bisabolene …
An IDS-Type Sesquiterpene Synthase Produces the Pheromone Precursor (Z)-alpha-Bisabolene in Nezara viridula.
Lancaster J, Lehner B, Khrimian A, Muchlinski A, Luck K, Köllner TG, Weber DC, Gundersen-Rindal DE, Tholl D. Lancaster J, et al. J Chem Ecol. 2019 Feb;45(2):187-197. doi: 10.1007/s10886-018-1019-0. Epub 2018 Sep 29. J Chem Ecol. 2019. PMID: 30267360
Here we provide evidence for de novo biosynthesis of the sex pheromone trans-/cis-(Z)-alpha-bisabolene epoxide of the Southern green stink bug, Nezara viridula. We show that an enzyme (NvTPS) related to isoprenyl diphosphate synthases (IDSs) of the core terpene meta …
Here we provide evidence for de novo biosynthesis of the sex pheromone trans-/cis-(Z)-alpha-bisabolene epoxide of the Southern …
Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002.
Davies FK, Work VH, Beliaev AS, Posewitz MC. Davies FK, et al. Front Bioeng Biotechnol. 2014 Jun 19;2:21. doi: 10.3389/fbioe.2014.00021. eCollection 2014. Front Bioeng Biotechnol. 2014. PMID: 25152894 Free PMC article.
The plant terpenoids limonene (C10H16) and alpha-bisabolene (C15H24) are hydrocarbon precursors to a range of industrially relevant chemicals. ...PCC 7002 to produce yields of 4 mg L(-1) limonene and 0.6 mg L(-1) alpha-bisabolene through heterologous e …
The plant terpenoids limonene (C10H16) and alpha-bisabolene (C15H24) are hydrocarbon precursors to a range of industrially rel …
69 results