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Determination of the biogenic amines and their major metabolites in single human brain tissue samples using a combined extraction procedure and high-performance liquid chromatography with electrochemical detection.
Herregodts P, Michotte Y, Ebinger G. Herregodts P, et al. J Chromatogr. 1985 Nov 29;345(1):33-42. doi: 10.1016/0378-4347(85)80132-8. J Chromatogr. 1985. PMID: 4086586
In a second step, 3-methoxytyramine and 5-hydroxytryptamine are isolated from the aqueous phase with di(2-ethylhexyl)phosphoric acid as counter-ion in chloroform. Dihydroxybenzylamine, isohomovanillic acid and 5-hydroxy-N-methyltryptamine are used as the internal st …
In a second step, 3-methoxytyramine and 5-hydroxytryptamine are isolated from the aqueous phase with di(2-ethylhexyl)phosphoric acid as coun …