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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1922 1
1934 1
1935 1
1938 1
1939 1
1940 1
1941 2
1945 5
1946 1
1947 4
1950 1
1951 4
1952 2
1953 3
1954 4
1955 2
1956 5
1957 1
1958 8
1959 5
1960 4
1961 3
1962 8
1963 4
1964 7
1965 10
1966 12
1967 12
1968 19
1969 9
1970 17
1971 15
1972 30
1973 73
1974 76
1975 88
1976 68
1977 63
1978 86
1979 76
1980 86
1981 100
1982 95
1983 123
1984 118
1985 170
1986 165
1987 165
1988 178
1989 193
1990 169
1991 147
1992 161
1993 173
1994 146
1995 127
1996 118
1997 130
1998 111
1999 97
2000 104
2001 94
2002 90
2003 65
2004 80
2005 77
2006 78
2007 84
2008 62
2009 61
2010 37
2011 44
2012 47
2013 48
2014 45
2015 47
2016 32
2017 29
2018 25
2019 24
2020 21
2021 28
2022 28
2023 22
2024 8

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4,667 results

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Page 1
Digitonin does not flip across cholesterol-poor membranes.
Fan HY, Heerklotz H. Fan HY, et al. J Colloid Interface Sci. 2017 Oct 15;504:283-293. doi: 10.1016/j.jcis.2017.05.034. Epub 2017 May 17. J Colloid Interface Sci. 2017. PMID: 28551523
Digitonin is commonly used to permeabilize cell membranes and solubilize membrane components. ...Beyond a digitonin/lipid ratio of 0.1 in the outer leaflet, digitonin micelles coexist with vesicles without solubilizing them-even at high digitonin conce …
Digitonin is commonly used to permeabilize cell membranes and solubilize membrane components. ...Beyond a digitonin/lipid rati …
Interactions of OSW-1 with Lipid Bilayers in Comparison with Digitonin and Soyasaponin.
Malabed R, Hanashima S, Murata M, Sakurai K. Malabed R, et al. Langmuir. 2020 Apr 7;36(13):3600-3610. doi: 10.1021/acs.langmuir.9b03957. Epub 2020 Mar 30. Langmuir. 2020. PMID: 32160747
Digitonin showed the highest binding affinity for the POPC-Chol membrane (K(app) = 0.38 muM(-1)) and the strongest membrane disruptivity in the bound saponin-lipid ratio at the point of 50% calcein leakage (r(50) = 0.47) occurrence. ...At the higher OSW-1 concentration (58 …
Digitonin showed the highest binding affinity for the POPC-Chol membrane (K(app) = 0.38 muM(-1)) and the strongest membrane disruptiv …
Radioimmunoassay of digitonin.
Brooker G, Fleming JW, Ross EM. Brooker G, et al. Biochim Biophys Acta. 1982 Feb 8;685(1):39-43. doi: 10.1016/0005-2736(82)90032-3. Biochim Biophys Acta. 1982. PMID: 7059589
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for binding to anti-digoxin antiserum. ...It should be useful for the monitoring of digitonin concentrations in solubilized biochemical prep …
We describe a radioimmunoassay for digitonin which utilizes the ability of digotonin to compete with 125I-labeled digoxigenin for bin …
Structural basis for sterol sensing by Scap and Insig.
Yan R, Cao P, Song W, Li Y, Wang T, Qian H, Yan C, Yan N. Yan R, et al. Cell Rep. 2021 Jun 29;35(13):109299. doi: 10.1016/j.celrep.2021.109299. Cell Rep. 2021. PMID: 34192549 Free article.
In this study, combining cryogenic electron microscopy (cryo-EM) analysis and artificial intelligence-facilitated structural prediction, we report the structure of the human Scap/Insig-2 complex purified in digitonin. The luminal domain loop 1 and a co-folded segment in lo …
In this study, combining cryogenic electron microscopy (cryo-EM) analysis and artificial intelligence-facilitated structural prediction, we …
Disordering Effects of Digitonin on Phospholipid Monolayers.
Orczyk M, Wojciechowski K, Brezesinski G. Orczyk M, et al. Langmuir. 2017 Apr 18;33(15):3871-3881. doi: 10.1021/acs.langmuir.6b04613. Epub 2017 Apr 5. Langmuir. 2017. PMID: 28333465
The observed increase in surface pressure (pi) suggests that digitonin can adsorb at the air/water interface, both bare and covered with the uncompressed phospholipid monolayers. ...Nevertheless, it should be noted that in no case did digitonin cause any disruptive …
The observed increase in surface pressure (pi) suggests that digitonin can adsorb at the air/water interface, both bare and covered w …
Solubilization, purification, and characterization of the hexameric form of phosphatidylserine synthase from Candida albicans.
Zhou Y, Syed JH, Semchonok DA, Wright E, Kyrilis FL, Hamdi F, Kastritis PL, Bruce BD, Reynolds TB. Zhou Y, et al. J Biol Chem. 2023 Jun;299(6):104756. doi: 10.1016/j.jbc.2023.104756. Epub 2023 Apr 26. J Biol Chem. 2023. PMID: 37116705 Free PMC article.
Blue native PAGE and immunoblot analysis revealed a single band corresponding to Cho1 in all detergent-solubilized fractions, while two bands were present in the SMA2000-solubilized fraction. Our enzymatic assay suggests that digitonin- or DDM-solubilized enzyme has the mo …
Blue native PAGE and immunoblot analysis revealed a single band corresponding to Cho1 in all detergent-solubilized fractions, while two band …
On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers.
Wojciechowski K, Orczyk M, Gutberlet T, Brezesinski G, Geue T, Fontaine P. Wojciechowski K, et al. Langmuir. 2016 Sep 6;32(35):9064-73. doi: 10.1021/acs.langmuir.6b01737. Epub 2016 Aug 26. Langmuir. 2016. PMID: 27518122
The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10(-4) M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and …
The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10(-4) M) by s …
Nuclear Transport Assays in Permeabilized Mouse Cortical Neurons.
Hayes LR, Duan L, Vidensky S, Kalab P. Hayes LR, et al. J Vis Exp. 2021 Jul 9;(173). doi: 10.3791/62710. J Vis Exp. 2021. PMID: 34309603
Permeabilized cell assays, in which the plasma membrane is selectively perforated by digitonin, are widely used to study passive and active nuclear transport in immortalized cell lines but have not been applied to neuronal cultures. In our initial attempts, we observed the …
Permeabilized cell assays, in which the plasma membrane is selectively perforated by digitonin, are widely used to study passive and …
Nuclear Protein Transport in Digitonin Permeabilized Cells.
Adam SA. Adam SA. Methods Mol Biol. 2016;1411:479-87. doi: 10.1007/978-1-4939-3530-7_29. Methods Mol Biol. 2016. PMID: 27147060
In this chapter, we describe the basic method to use digitonin permeabilized cells to reconstitute the transport of proteins containing nuclear localization signals into the nucleus. The assay requires only a target cell line that can be permeabilized with digitonin …
In this chapter, we describe the basic method to use digitonin permeabilized cells to reconstitute the transport of proteins containi …
Control of exocytosis from adrenal chromaffin cells.
Holz RW. Holz RW. Cell Mol Neurobiol. 1988 Sep;8(3):259-68. doi: 10.1007/BF00711168. Cell Mol Neurobiol. 1988. PMID: 3066486 Free article. Review.
Calcium-dependent exocytosis of catecholamines from intact and digitonin-permeabilized bovine adrenal chromaffin cells was investigated. 2. 45Ca2+ uptake and secretion induced by nicotinic stimulation or depolarization in intact cells were closely correlated. ...The plasma …
Calcium-dependent exocytosis of catecholamines from intact and digitonin-permeabilized bovine adrenal chromaffin cells was investigat …
4,667 results