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Page 1
Binary interactions of troponin subunits.
Ingraham RH, Swenson CA. Ingraham RH, et al. J Biol Chem. 1984 Aug 10;259(15):9544-8. J Biol Chem. 1984. PMID: 6204984 Free article.
The subunits were labeled with extrinsic fluorescence probes, either 5-(iodoacetamido)eosin (IAE) or dansylaziridine (DANZ), and the binding was detected by enhancement or quenching of the probe fluorescence. ...
The subunits were labeled with extrinsic fluorescence probes, either 5-(iodoacetamido)eosin (IAE) or dansylaziridine (DANZ), and the …
Determination of the free-energy coupling for binding of calcium ions and troponin I to calmodulin.
Keller CH, Olwin BB, LaPorte DC, Storm DR. Keller CH, et al. Biochemistry. 1982 Jan 5;21(1):156-62. doi: 10.1021/bi00530a027. Biochemistry. 1982. PMID: 7059575
The dissociation constant for the Ca2+4.calmodulin and troponin I complex was also determined directly by monitoring fluorescence intensity changes accompanying complex formation between Ca2+4.CaM and N-dansylaziridine-troponin I, a fluorescent derivative of troponin I. Ca …
The dissociation constant for the Ca2+4.calmodulin and troponin I complex was also determined directly by monitoring fluorescence intensity …
Fluorescence titration and fluorescence stopped-flow studies on skeletal troponin C labeled with fluorescent maleimide reagent or dansylaziridine.
Iio T, Kondo H. Iio T, et al. J Biochem. 1981 Jul;90(1):163-75. doi: 10.1093/oxfordjournals.jbchem.a133446. J Biochem. 1981. PMID: 7287674 Free article.
These dye-protein conjugates do not show fluorescence intensity change upon Ca2+ binding to the low affinity Ca2+-binding sites (sites I and II). Dansylaziridine (DANZ)-labeled TN-C shows more than 100% fluorescence increase upon Ca2+ binding to the low affinity Ca2+-bindi …
These dye-protein conjugates do not show fluorescence intensity change upon Ca2+ binding to the low affinity Ca2+-binding sites (sites I and …
Thiol-specific probes indicate that the beta-chain of platelet glycoprotein Ib is a transmembrane protein with a reactive endofacial sulfhydryl group.
Kalomiris EL, Coller BS. Kalomiris EL, et al. Biochemistry. 1985 Sep 24;24(20):5430-6. doi: 10.1021/bi00341a022. Biochemistry. 1985. PMID: 2934089
Intact platelets were reacted with 1-10 mM monobromobimane or monobromo(trimethylammonio)bimane or 50-100 microM N-dansylaziridine for 30-60 min at 37 degree C. The platelets were then washed, solubilized in 1% Triton X-100, and analyzed by nonreduced-reduced polyacrylamid …
Intact platelets were reacted with 1-10 mM monobromobimane or monobromo(trimethylammonio)bimane or 50-100 microM N-dansylaziridine fo …
Chemical modification of rabbit IgG with N-dansylaziridine. Investigation of the properties of dansylated antibodies.
Pikuleva IA, Turko IV, Adamovich TB, Chashchin VL. Pikuleva IA, et al. Mol Immunol. 1991 Apr-May;28(4-5):311-8. doi: 10.1016/0161-5890(91)90142-7. Mol Immunol. 1991. PMID: 2062314
To elucidate the effect of antigen binding fluorescent thiol reagent, N-dansylaziridine (DAZ), which is sensitive to the changes in the microenvironment, was used for modification of rabbit IgG hinge region cysteine residue. ...
To elucidate the effect of antigen binding fluorescent thiol reagent, N-dansylaziridine (DAZ), which is sensitive to the changes in t …
Comparison of the kinetic properties of troponin-C and dansylaziridine-labeled troponin-C1.
Iio T, Kondo H. Iio T, et al. J Biochem. 1980 Aug;88(2):547-56. doi: 10.1093/oxfordjournals.jbchem.a133002. J Biochem. 1980. PMID: 7419512 Free article.
Comparison of the kinetic properties of troponin-C and dansylaziridine-labeled troponin-C revealed that the calcium ion binding and removal reactions with the low affinity Ca2+-binding sites (sites I and II) and the resultant local conformational change are rapid processes …
Comparison of the kinetic properties of troponin-C and dansylaziridine-labeled troponin-C revealed that the calcium ion binding and r …
Interaction of a fluorescent N-dansylaziridine derivative of troponin I with calmodulin in the absence and presence of calcium.
Olwin BB, Keller CH, Storm DR. Olwin BB, et al. Biochemistry. 1982 Oct 26;21(22):5669-75. doi: 10.1021/bi00265a043. Biochemistry. 1982. PMID: 6293554
Rabbit skeletal muscle troponin I was covalently labeled with N-dansylaziridine, resulting in a fluorescent labeled protein. This derivative (DANZTnI) and native troponin I (TnI) inhibited calmodulin (CaM) stimulation of bovine heart Ca2+-sensitive cyclic nucleodite phosph …
Rabbit skeletal muscle troponin I was covalently labeled with N-dansylaziridine, resulting in a fluorescent labeled protein. This der …
Effect of rigor and cycling cross-bridges on the structure of troponin C and on the Ca2+ affinity of the Ca2+-specific regulatory sites in skinned rabbit psoas fibers.
Güth K, Potter JD. Güth K, et al. J Biol Chem. 1987 Oct 5;262(28):13627-35. J Biol Chem. 1987. PMID: 3654633 Free article.
Intrinsic troponin C (TnC) was extracted from small bundles of rabbit psoas fibers and replaced with TnC labeled with dansylaziridine (5-dimethylaminonaphthalene-1-sulfonyl). The flourescence of incorporated dansylaziridine-labeled TnC was enhanced by the binding of …
Intrinsic troponin C (TnC) was extracted from small bundles of rabbit psoas fibers and replaced with TnC labeled with dansylaziridine …
47 results