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A general RNA-capping ribozyme retains stereochemistry during cap exchange.
Zaher HS, Unrau PJ. Zaher HS, et al. J Am Chem Soc. 2006 Oct 25;128(42):13894-900. doi: 10.1021/ja0639822. J Am Chem Soc. 2006. PMID: 17044717
A ribozyme prepared with an Rp adenosine(5')alpha-thiotetraphosphate cap was found to exchange this cap for an Rp 4-thiouridine(5')alpha-thiotetraphosphate cap when incubated with 4-thiouridine triphosphate. The same Rp capped construct, when incubated with [ …
A ribozyme prepared with an Rp adenosine(5')alpha-thiotetraphosphate cap was found to exchange this cap for an Rp 4-thiouridine(5')alpha-thi …
Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analogues.
Tanner NK, Hanna MM, Abelson J. Tanner NK, et al. Biochemistry. 1988 Nov 29;27(24):8852-61. doi: 10.1021/bi00424a025. Biochemistry. 1988. PMID: 2853971
When irradiated with long ultraviolet light, RNA containing these analogues can form specific covalent bonds with associated proteins. In this paper, we show that 4-thiouridine triphosphate and 5-bromouridine triphosphate were readily incorporated into a prec …
When irradiated with long ultraviolet light, RNA containing these analogues can form specific covalent bonds with associated proteins. In th …
The environment of 5S rRNA in the ribosome: cross-links to 23S rRNA from sites within helices II and III of the 5S molecule.
Osswald M, Brimacombe R. Osswald M, et al. Nucleic Acids Res. 1999 Jun 1;27(11):2283-90. doi: 10.1093/nar/27.11.2283. Nucleic Acids Res. 1999. PMID: 10325415 Free PMC article.
The central fragment, comprising residues 33-71 of the molecule, was transcribed in the presence of 4-thiouridine triphosphate together with [32P]UTP. The three transcripts were ligated together, yielding a 5S rRNA analogue carrying 4-thiouridine residues at …
The central fragment, comprising residues 33-71 of the molecule, was transcribed in the presence of 4-thiouridine triphosph …
Yeast RNA polymerase I. Derivatization of the 190 and 135 subunits by 4-thiouridine monophosphate positioned uniquely at the 3' terminus of an enzyme-bound 32P-containing transcript initiated by a triribonucleotide primer on synthetic single-stranded DNA.
Kelly S, Sheng N, Dennis D. Kelly S, et al. J Biol Chem. 1990 May 15;265(14):7787-92. J Biol Chem. 1990. PMID: 2159457 Free article.
The templates were designed to limit the incorporation of a photoprobe, 4-thiouridine triphosphate, to a single unique position at the 3' terminus of the product RNA (position 12, 13, 14, or 15). ...
The templates were designed to limit the incorporation of a photoprobe, 4-thiouridine triphosphate, to a single unique …
11 results