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Page 1
SPASM and twitch domains in S-adenosylmethionine (SAM) radical enzymes.
Grell TA, Goldman PJ, Drennan CL. Grell TA, et al. J Biol Chem. 2015 Feb 13;290(7):3964-71. doi: 10.1074/jbc.R114.581249. Epub 2014 Dec 4. J Biol Chem. 2015. PMID: 25477505 Free PMC article. Review.
The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non-SPASM proteins, butirosin biosynthetic enzyme 2-deoxy-scyllo-inosamine dehydrogenase (BtrN) and molybdenum cofa …
The first structure of a SPASM-containing enzyme, anaerobic sulfatase-maturating enzyme (anSME), revealed unexpected similarities to two non …
Characterization of L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from Streptomyces tenebrarius.
Kharel MK, Subba B, Lee HC, Liou K, Sohng JK. Kharel MK, et al. Bioorg Med Chem Lett. 2005 Jan 3;15(1):89-92. doi: 10.1016/j.bmcl.2004.10.028. Bioorg Med Chem Lett. 2005. PMID: 15582417
A gene encoding L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from the tobramycin producer Streptomyces tenebrarius was expressed heterologously in Escherichia coli. The conversions of 2-deoxy-scyllo-inosose to 2-deoxy-scyllo-inosamine and …
A gene encoding L-glutamine:2-deoxy-scyllo-inosose aminotransferase (tbmB) from the tobramycin producer Streptomyces tenebrarius was express …
Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.
Tamegai H, Nango E, Kuwahara M, Yamamoto H, Ota Y, Kuriki H, Eguchi T, Kakinuma K. Tamegai H, et al. J Antibiot (Tokyo). 2002 Aug;55(8):707-14. doi: 10.7164/antibiotics.55.707. J Antibiot (Tokyo). 2002. PMID: 12374384 Free article.
The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-in …
The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2 …
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K. Kudo F, et al. J Antibiot (Tokyo). 2005 Dec;58(12):766-74. doi: 10.1038/ja.2005.104. J Antibiot (Tokyo). 2005. PMID: 16506694
NeoC was functionally characterized as 2-deoxy-scyllo-inosose synthase, which catalyzes the carbocycle formation from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose. Further, NeoA appeared to catalyze the oxidation of 2-deoxy-scyllo-inosamine (DOIA) w …
NeoC was functionally characterized as 2-deoxy-scyllo-inosose synthase, which catalyzes the carbocycle formation from D-glucose-6-phosphate …
Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis.
Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB. Popovic B, et al. Proteins. 2006 Oct 1;65(1):220-30. doi: 10.1002/prot.21076. Proteins. 2006. PMID: 16894611
The aminotransferase (BtrR), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-DOS)-containing aminoglycoside antibiotic produced by Bacillus circulans, catalyses the pyridoxal phosphate (PLP)-dependent transamination reaction both of 2-deoxy-scyllo-inos …
The aminotransferase (BtrR), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-DOS)-containing aminoglycoside anti …
Mechanistic study on the reaction of a radical SAM dehydrogenase BtrN by electron paramagnetic resonance spectroscopy.
Yokoyama K, Ohmori D, Kudo F, Eguchi T. Yokoyama K, et al. Biochemistry. 2008 Aug 26;47(34):8950-60. doi: 10.1021/bi800509x. Epub 2008 Aug 2. Biochemistry. 2008. PMID: 18672902
BtrN is a radical SAM ( S-adenosyl- l-methionine) enzyme that catalyzes the oxidation of 2-deoxy- scyllo-inosamine (DOIA) into 3-amino-2,3-dideoxy- scyllo-inosose (amino-DOI) during the biosynthesis of 2-deoxystreptamine (DOS) in the butirosin producer …
BtrN is a radical SAM ( S-adenosyl- l-methionine) enzyme that catalyzes the oxidation of 2-deoxy- scyllo-inosamine …
X-ray analysis of butirosin biosynthetic enzyme BtrN redefines structural motifs for AdoMet radical chemistry.
Goldman PJ, Grove TL, Booker SJ, Drennan CL. Goldman PJ, et al. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):15949-54. doi: 10.1073/pnas.1312228110. Epub 2013 Sep 18. Proc Natl Acad Sci U S A. 2013. PMID: 24048029 Free PMC article.
The 2-deoxy-scyllo-inosamine (DOIA) dehydrogenases are key enzymes in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. ...
The 2-deoxy-scyllo-inosamine (DOIA) dehydrogenases are key enzymes in the biosynthesis of 2-deoxystreptamine-con …
Electrochemical Resolution of the [4Fe-4S] Centers of the AdoMet Radical Enzyme BtrN: Evidence of Proton Coupling and an Unusual, Low-Potential Auxiliary Cluster.
Maiocco SJ, Grove TL, Booker SJ, Elliott SJ. Maiocco SJ, et al. J Am Chem Soc. 2015 Jul 15;137(27):8664-7. doi: 10.1021/jacs.5b03384. Epub 2015 Jun 25. J Am Chem Soc. 2015. PMID: 26088836
Here, BtrN, an AdoMet radical dehydrogenase that catalyzes the two-electron oxidation of 2-deoxy-scyllo-inosamine to amino-dideoxy-scyllo-inosose, an intermediate in the biosynthesis of 2-deoxystreptamine antibiotics, is examined through direct electro …
Here, BtrN, an AdoMet radical dehydrogenase that catalyzes the two-electron oxidation of 2-deoxy-scyllo-inosamine …
Characterization and mechanistic study of a radical SAM dehydrogenase in the biosynthesis of butirosin.
Yokoyama K, Numakura M, Kudo F, Ohmori D, Eguchi T. Yokoyama K, et al. J Am Chem Soc. 2007 Dec 12;129(49):15147-55. doi: 10.1021/ja072481t. Epub 2007 Nov 15. J Am Chem Soc. 2007. PMID: 18001019
Its gene disruption in the butirosin producer Bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (DOIA) and 2-deoxystreptamine (DOS). Further, in vitro assay of the overexpressed enzyme revealed that …
Its gene disruption in the butirosin producer Bacillus circulans caused the interruption of the biosynthetic pathway between 2-deo …
14 results