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Labeling the (Ca(2+)-Mg2+)-ATPase of sarcoplasmic reticulum at Glu-439 with 5-(bromomethyl)fluorescein.
Stefanova HI, Mata AM, Gore MG, East JM, Lee AG. Stefanova HI, et al. Biochemistry. 1993 Jun 15;32(23):6095-103. doi: 10.1021/bi00074a022. Biochemistry. 1993. PMID: 8099497
The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoichiometry of one label per ATPase molecule was found, which was unaffected by the presence of ATP. ...
The (Ca(2+)-Mg2+)-ATPase of skeletal muscle sarcoplasmic reticulum was labeled with 5-(bromomethyl)fluorescein. A stoic …
5-Bromomethyl fluorescein (5-BMF) for derivatization of carboxyl containing analytes for use with laser-induced fluorescence detection.
Mukherjee PS, DeSilva KH, Karnes HT. Mukherjee PS, et al. Pharm Res. 1995 Jun;12(6):930-6. doi: 10.1023/a:1016285709682. Pharm Res. 1995. PMID: 7667203
PURPOSE: 5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. ...
PURPOSE: 5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent fo …
Metal ion-catalyzed nucleic acid alkylation and fragmentation.
Browne KA. Browne KA. J Am Chem Soc. 2002 Jul 10;124(27):7950-62. doi: 10.1021/ja017746x. J Am Chem Soc. 2002. PMID: 12095339
In model studies with 26 nucleotide-long RNA and DNA oligomers using ultraviolet/visible and fluorescence spectroscopies as well as high-pressure liquid chromatography and mass spectrometry, addition of both alkylating agents (5-(bromomethyl)fluorescein, 5- o …
In model studies with 26 nucleotide-long RNA and DNA oligomers using ultraviolet/visible and fluorescence spectroscopies as well as high-pre …
High-throughput capillary electrophoretic method for determination of total aminothiols in plasma and urine.
Lochman P, Adam T, Friedecký D, Hlídková E, Skopková Z. Lochman P, et al. Electrophoresis. 2003 Apr;24(7-8):1200-7. doi: 10.1002/elps.200390154. Electrophoresis. 2003. PMID: 12707912
Samples were reduced with tris(carboxyethyl)phosphine and labeled with 5-(bromomethyl)fluorescein. Capillary electrophoretic separations were performed in 60 mmol/L borate - 15 mmol/L sodium dodecyl sulfate - 2-amino-2-methyl-1-propanol, pH 10.0, with laser-i …
Samples were reduced with tris(carboxyethyl)phosphine and labeled with 5-(bromomethyl)fluorescein. Capillary electropho …
Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag.
Lee JJ, Son J, Ha HH, Chang YT. Lee JJ, et al. Mol Biosyst. 2011 Apr;7(4):1270-6. doi: 10.1039/c0mb00327a. Epub 2011 Feb 10. Mol Biosyst. 2011. PMID: 21311795
We report a novel protein tagging method that provides a convenient and specific fluorescent labeling of membrane proteins. omega tag is developed by employing a mammalian enzyme glutathione sulfur-transferase omega 1 (GSTO1) and its partner dye, 5-bromomethyl fl …
We report a novel protein tagging method that provides a convenient and specific fluorescent labeling of membrane proteins. omega tag is dev …
Quantification of carboxyl groups in carbodiimide cross-linked collagen sponges.
Everaerts F, Torrianni M, Hendriks M, Feijen J. Everaerts F, et al. J Biomed Mater Res A. 2007 Dec 15;83(4):1176-1183. doi: 10.1002/jbm.a.31398. J Biomed Mater Res A. 2007. PMID: 17595021
Reconstituted dermal bovine collagen matrices (RDBC) were cross-linked with a water soluble CDI and N-hydroxysuccinimide (NHS) and residual carboxyl groups were labeled using 5-bromomethyl fluorescein. Subsequently, the fluorescent label was released by mild …
Reconstituted dermal bovine collagen matrices (RDBC) were cross-linked with a water soluble CDI and N-hydroxysuccinimide (NHS) and residual …
Localization of the hinge region of the Ca(2+)-ATPase of sarcoplasmic reticulum using resonance energy transfer.
Baker KJ, East JM, Lee AG. Baker KJ, et al. Biochim Biophys Acta. 1994 Jun 1;1192(1):53-60. doi: 10.1016/0005-2736(94)90142-2. Biochim Biophys Acta. 1994. PMID: 8204650
Resonance energy transfer has been used to measure the distance between Cys-670/Cys-674 and Glu-439 labelled with 5-(bromomethyl)fluorescein as 40 A. The height of Cys-670/Cys-674 above the phospholipid/water interface has been measured by resonance energy tr …
Resonance energy transfer has been used to measure the distance between Cys-670/Cys-674 and Glu-439 labelled with 5-(bromomethyl …
11 results