Primary erythroblast culture response to cytokine-induced increase in fetal hemoglobin
Summary:
Analysis of primary CD34+ hematopoietic progenitor cells, cultured in low-HbF (EPO alone) or high-HbF conditions (EPO, SCF, and TGFbeta-1), on days 7 (proerythroblasts) and 14 (mature erythrocytes). Results provide insight into molecular mechanisms underlying cytokine-induced increases in HbF.
GPL570:
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Citations:
Sripichai O, Kiefer CM, Bhanu NV, Tanno T et al. Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming. Blood 2009 Sep 10;114(11):2299-306. PMID: 19597182
Tanno T, Bhanu NV, Oneal PA, Goh SH et al. High levels of GDF15 in thalassemia suppress expression of the iron regulatory protein hepcidin. Nat Med 2007 Sep;13(9):1096-101. PMID: 17721544
