Table 6Analytic validity of simultaneous detection (multiplex) of FVL and prothrombin G20210A mutations

Experimental TestFRET + melting Curve Analysis (Light Cycler)Mutagenically Separated PCR Followed by Gel ElectrophoresisMultiplex PCR- RFLPMultiplex PCR – Reverse Hybridization Line Probe AssayMultiplex AS-PCR - Ion-Pair Reversed Phase HPLCMultiplex PCR with ASO Hybridization Using the 5′ Nuclease Assay
Reference StandardPCR-RFLPPCR-RFLPPCR-RFLP or Light CyclerPCR-RFLPPCR-RFLPPCR-RFLP
Studies (ref. no.)van den Bergh, 200092
Hobson- Peters, 200593
Ameziane, 200394
Endler, 200156Baris, 200495
Angeline, 200551
Huber, 200096
Lucotte, 200397
Koksal, 200757
Leyte, 200086Nietzel, 200399Ugozzoli, 2004100
Total Population*47 + 43 + 200153408 + 72 + 205 + 175 + 10499> 10052
Concordance100%100%100%100%100%100%
Source of Zygosity Discordance**NANANANANANA
Did Repeating Resolve the Discordance**?NANANANANANA
*

The order of the counts corresponds to the order of studies in the row above;

**

if <100% concordance

ASO = allele-specific oligonucleotide; AS-PCR = allele-specific PCR; FRET = fluorescence resonance energy transfer; HPLC = high performance (pressure) liquid chromatography; NA = not applicable; PCR = polymerase chain reaction; RFLP = restriction fragment length polymorphism

From: 3, Results

Cover of Outcomes of Genetic Testing in Adults with a History of Venous Thromboembolism
Outcomes of Genetic Testing in Adults with a History of Venous Thromboembolism.
Evidence Reports/Technology Assessments, No. 180.
Segal JB, Brotman DJ, Emadi A, et al.

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