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Gene Ther. 2010 Jun;17(6):811-4. doi: 10.1038/gt.2010.22. Epub 2010 Mar 4.

Cutaneous vaccination using microneedles coated with hepatitis C DNA vaccine.

Author information

1
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Abstract

The skin is potentially an excellent organ for vaccine delivery because of accessibility and the presence of immune cells. However, no simple and inexpensive cutaneous vaccination method is available. Micron-scale needles coated with DNA were tested as a simple, inexpensive device for skin delivery. Vaccination with a plasmid encoding hepatitis C virus nonstructural 3/4A protein using microneedles effectively primed specific cytotoxic T lymphocytes (CTLs). Importantly, the minimally invasive microneedles were as efficient in priming CTLs as more complicated or invasive delivery techniques, such as gene gun and hypodermic needles. Thus, microneedles may offer a promising technology for DNA vaccination.

PMID:
20200562
PMCID:
PMC2914565
DOI:
10.1038/gt.2010.22
[Indexed for MEDLINE]
Free PMC Article

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