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Int Urol Nephrol. 2016 Dec;48(12):2051-2059. Epub 2016 Aug 29.

Nested case-control study reveals increased levels of urinary proteins from human kidney toxicity panels in women predicted to develop preeclampsia.

Author information

1
Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6 Ejido la Escondida, Zacatecas, CP 98160, Mexico.
2
Catedras Program, Consejo Nacional de Ciencia y Tecnología (CONACYT), Ciudad de Mexico, Mexico.
3
Bioengineering Laboratory, Centro de Investigacion e Innovacion Tecnologica Industrial, Unidad Academica de Ingenieria Electrica, Universidad Autonoma de Zacatecas, Zacatecas, Mexico.
4
Grupo de Investigacion Regional Emergente (GIRE), Zacatecas, Mexico.
5
Facultad de Medicina, Universidad Autonoma de Colima, Colima, Mexico.
6
Servicios de Salud del Estado de Colima, Instituto Estatal de Cancerologia, Colima, Mexico.
7
Departamento de Genetica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon, Monterrey, Nuevo Leon, Mexico.
8
Molecular Medicine Laboratory, Unidad Academica de Medicina Humana y Ciencias de la Salud, Universidad Autonoma de Zacatecas, Carretera Zacatecas-Guadalajara Km 6 Ejido la Escondida, Zacatecas, CP 98160, Mexico. margaritamf@uaz.edu.mx.
9
Bioengineering Laboratory, Centro de Investigacion e Innovacion Tecnologica Industrial, Unidad Academica de Ingenieria Electrica, Universidad Autonoma de Zacatecas, Zacatecas, Mexico. margaritamf@uaz.edu.mx.
10
Grupo de Investigacion Regional Emergente (GIRE), Zacatecas, Mexico. margaritamf@uaz.edu.mx.

Abstract

PURPOSE:

The aim of this study was to evaluate the usefulness of urine concentrations of 12 proteins as a risk parameter for developing preeclampsia (PE).

METHODS:

A nested case-control study was designed to determine protein concentrations in urine from women predicted to develop PE (WPD-PE) and normotensive pregnancies (controls). Protein profiles were determined at 12, 16 and 20 gestational weeks (GW) using the Bio-Plex Pro human kidney toxicity Panel 1 and Panel 2 (Bio-Rad). Receiver operating characteristic (ROC) curve analyses were performed. Correlations between proteins and clinical parameters at the time of PE diagnosis were also assessed.

RESULTS:

Significant differences were observed in urine cystatin C (Cys C) levels at 16 and 20 GW and clusterin at 20 GW between WPD-PE and controls (P < 0.05). ROC analysis revealed that Cys C at 16 GW had the highest area under the ROC curve (0.758). At 16 GW, patients with urine Cys C levels above 73.7 ng/mL had eightfold increased odds for developing PE (odds ratio 7.92; 95 % CI 1.3-47.5; P = 0.027). A positive correlation was found between urinary Cys C (at 16 and 20 GW) and leukocyte counts, total proteins, aspartate aminotransferase, alanine aminotransferase, bilirubin and lactate dehydrogenase at the time of PE diagnosis (P value < 0.05).

CONCLUSIONS:

Urinary Cys C and clusterin showed predictive value for PE development in our cohort. Further studies are needed to validate their use as predictive biomarkers for PE and/or their participation in PE pathogenesis.

KEYWORDS:

Biomarkers; Clusterin; Cystatin C; Preeclampsia; Urine

PMID:
27571961
DOI:
10.1007/s11255-016-1397-6
[Indexed for MEDLINE]

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