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Clin Infect Dis. 2012 May;54 Suppl 4:S273-9. doi: 10.1093/cid/cir982.

Evaluation of in-house genotyping assay performance using dried blood spot specimens in the Global World Health Organization laboratory network.

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1
Data First Consulting, Belmont, California 94002, USA. nparkin34@gmail.com

Abstract

In resource-limited settings, there is increased demand for human immunodeficiency virus type 1 drug resistance testing. Because preservation of plasma specimens is often not feasible in resource-limited settings, use of dried blood spots (DBSs) is being adopted. We used 2 panels of DBSs for genotyping assay validation and proficiency testing in selected laboratories in the World Health Organization laboratory network in 14 countries. An amplification sensitivity of 1000 copies/mL was achieved by 2 laboratories. Reproducibility and accuracy of nucleotide sequence determination and resistance-associated mutation identification from DBSs was similar to that previously determined for plasma. International shipping at ambient temperature had no significant effect on amplification success. These studies indicate that DBS-based genotyping is equally reproducible and reliable, although slightly less sensitive, compared with plasma.

PMID:
22544187
PMCID:
PMC3338305
DOI:
10.1093/cid/cir982
[Indexed for MEDLINE]
Free PMC Article
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