Format

Send to

Choose Destination

See 1 citation found by title matching your search:

Nano Lett. 2008 Jan;8(1):350-61. Epub 2007 Dec 13.

Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.

Author information

1
CRO-IRCCS National Cancer Institute, Aviano, Italy.

Abstract

Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

PMID:
18076201
PMCID:
PMC2877922
DOI:
10.1021/nl072174l
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center