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A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples.

Battin C, Hennig A, Mayrhofer P, Kunert R, Zlabinger GJ, Steinberger P, Paster W.

PLoS One. 2017 May 24;12(5):e0178220. doi: 10.1371/journal.pone.0178220. eCollection 2017.


Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression.

Mayrhofer P, Kunert R.

Methods Mol Biol. 2017;1603:57-69. doi: 10.1007/978-1-4939-6972-2_4.


Biphasic cultivation strategy to avoid Epo-Fc aggregation and optimize protein expression.

Kaisermayer C, Reinhart D, Gili A, Chang M, Aberg PM, Castan A, Kunert R.

J Biotechnol. 2016 Jun 10;227:3-9. doi: 10.1016/j.jbiotec.2016.03.054. Epub 2016 Apr 2.


Advances in recombinant antibody manufacturing.

Kunert R, Reinhart D.

Appl Microbiol Biotechnol. 2016 Apr;100(8):3451-61. doi: 10.1007/s00253-016-7388-9. Epub 2016 Mar 3. Review.


Proteomic differences in recombinant CHO cells producing two similar antibody fragments.

Sommeregger W, Mayrhofer P, Steinfellner W, Reinhart D, Henry M, Clynes M, Meleady P, Kunert R.

Biotechnol Bioeng. 2016 Sep;113(9):1902-12. doi: 10.1002/bit.25957. Epub 2016 Mar 16.


Antibody humanization by molecular dynamics simulations-in-silico guided selection of critical backmutations.

Margreitter C, Mayrhofer P, Kunert R, Oostenbrink C.

J Mol Recognit. 2016 Jun;29(6):266-75. doi: 10.1002/jmr.2527. Epub 2016 Jan 8.


Heterologous protein production using euchromatin-containing expression vectors in mammalian cells.

Zboray K, Sommeregger W, Bogner E, Gili A, Sterovsky T, Fauland K, Grabner B, Stiedl P, Moll HP, Bauer A, Kunert R, Casanova E.

Nucleic Acids Res. 2015 Sep 18;43(16):e102. doi: 10.1093/nar/gkv475. Epub 2015 May 14.


Benchmarking of commercially available CHO cell culture media for antibody production.

Reinhart D, Damjanovic L, Kaisermayer C, Kunert R.

Appl Microbiol Biotechnol. 2015 Jun;99(11):4645-57. doi: 10.1007/s00253-015-6514-4. Epub 2015 Apr 7.


Improved protocol for rapid identification of certain spa types using high resolution melting curve analysis.

Mayerhofer B, Stöger A, Pietzka AT, Fernandez HL, Prewein B, Sorschag S, Kunert R, Allerberger F, Ruppitsch W.

PLoS One. 2015 Mar 13;10(3):e0116713. doi: 10.1371/journal.pone.0116713. eCollection 2015.


Introduction of germline residues improves the stability of anti-HIV mAb 2G12-IgM.

Chromikova V, Mader A, Hofbauer S, Göbl C, Madl T, Gach JS, Bauernfried S, Furtmüller PG, Forthal DN, Mach L, Obinger C, Kunert R.

Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1536-44. doi: 10.1016/j.bbapap.2015.02.018. Epub 2015 Mar 5.


Upstream and downstream processing of recombinant IgA.

Reinhart D, Kunert R.

Biotechnol Lett. 2015 Feb;37(2):241-51. doi: 10.1007/s10529-014-1686-z. Epub 2014 Sep 26. Review.


Accurate comparison of antibody expression levels by reproducible transgene targeting in engineered recombination-competent CHO cells.

Mayrhofer P, Kratzer B, Sommeregger W, Steinfellner W, Reinhart D, Mader A, Turan S, Qiao J, Bode J, Kunert R.

Appl Microbiol Biotechnol. 2014 Dec;98(23):9723-33. doi: 10.1007/s00253-014-6011-1. Epub 2014 Aug 27.


Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.

Maccani A, Hackl M, Leitner C, Steinfellner W, Graf AB, Tatto NE, Karbiener M, Scheideler M, Grillari J, Mattanovich D, Kunert R, Borth N, Grabherr R, Ernst W.

Appl Microbiol Biotechnol. 2014 Sep;98(17):7535-48. doi: 10.1007/s00253-014-5911-4. Epub 2014 Jul 23.


Generation of a canine anti-EGFR (ErbB-1) antibody for passive immunotherapy in dog cancer patients.

Singer J, Fazekas J, Wang W, Weichselbaumer M, Matz M, Mader A, Steinfellner W, Meitz S, Mechtcheriakova D, Sobanov Y, Willmann M, Stockner T, Spillner E, Kunert R, Jensen-Jarolim E.

Mol Cancer Ther. 2014 Jul;13(7):1777-90. doi: 10.1158/1535-7163.MCT-13-0288. Epub 2014 Apr 22.


Evaluating the bottlenecks of recombinant IgM production in mammalian cells.

Chromikova V, Mader A, Steinfellner W, Kunert R.

Cytotechnology. 2015 Mar;67(2):343-56. doi: 10.1007/s10616-014-9693-4. Epub 2014 Mar 11.


In search of expression bottlenecks in recombinant CHO cell lines--a case study.

Reinhart D, Sommeregger W, Debreczeny M, Gludovacz E, Kunert R.

Appl Microbiol Biotechnol. 2014 Jul;98(13):5959-65. doi: 10.1007/s00253-014-5584-z. Epub 2014 Feb 21.


Novel human renal proximal tubular cell line for the production of complex proteins.

Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R.

J Biotechnol. 2014 Apr 20;176:29-39. doi: 10.1016/j.jbiotec.2014.02.001. Epub 2014 Feb 16.


Novel bifunctional single-chain variable antibody fragments to enhance virolysis by complement: generation and proof-of-concept.

Huber G, Bánki Z, Kunert R, Stoiber H.

Biomed Res Int. 2014;2014:971345. doi: 10.1155/2014/971345. Epub 2014 Jan 12.


Stable overexpression of miR-17 enhances recombinant protein production of CHO cells.

Jadhav V, Hackl M, Klanert G, Hernandez Bort JA, Kunert R, Grillari J, Borth N.

J Biotechnol. 2014 Apr 10;175:38-44. doi: 10.1016/j.jbiotec.2014.01.032. Epub 2014 Feb 8.


Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteins.

Maccani A, Landes N, Stadlmayr G, Maresch D, Leitner C, Maurer M, Gasser B, Ernst W, Kunert R, Mattanovich D.

Biotechnol J. 2014 Apr;9(4):526-37. doi: 10.1002/biot.201300305. Epub 2014 Jan 27.

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