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Items: 1 to 20 of 35


Confirmation of intersubunit connectivity and topology of designed protein complexes by native MS.

Sahasrabuddhe A, Hsia Y, Busch F, Sheffler W, King NP, Baker D, Wysocki VH.

Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):1268-1273. doi: 10.1073/pnas.1713646115. Epub 2018 Jan 19.


Evolution of a designed protein assembly encapsulating its own RNA genome.

Butterfield GL, Lajoie MJ, Gustafson HH, Sellers DL, Nattermann U, Ellis D, Bale JB, Ke S, Lenz GH, Yehdego A, Ravichandran R, Pun SH, King NP, Baker D.

Nature. 2017 Dec 21;552(7685):415-420. doi: 10.1038/nature25157. Epub 2017 Dec 13.


Multivalent Display of Antifreeze Proteins by Fusion to Self-Assembling Protein Cages Enhances Ice-Binding Activities.

Phippen SW, Stevens CA, Vance TD, King NP, Baker D, Davies PL.

Biochemistry. 2016 Dec 13;55(49):6811-6820. Epub 2016 Nov 29.


Designed proteins induce the formation of nanocage-containing extracellular vesicles.

Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King NP, Sundquist WI.

Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub 2016 Nov 30.


Corrigendum: Design of a hyperstable 60-subunit protein icosahedron.

Hsia Y, Bale JB, Gonen S, Shi D, Sheffler W, Fong KK, Nattermann U, Xu C, Huang PS, Ravichandran R, Yi S, Davis TN, Gonen T, King NP, Baker D.

Nature. 2016 Dec 1;540(7631):150. doi: 10.1038/nature20108. Epub 2016 Oct 19. No abstract available.


Accurate design of megadalton-scale two-component icosahedral protein complexes.

Bale JB, Gonen S, Liu Y, Sheffler W, Ellis D, Thomas C, Cascio D, Yeates TO, Gonen T, King NP, Baker D.

Science. 2016 Jul 22;353(6297):389-94. doi: 10.1126/science.aaf8818.


Design of a hyperstable 60-subunit protein dodecahedron. [corrected].

Hsia Y, Bale JB, Gonen S, Shi D, Sheffler W, Fong KK, Nattermann U, Xu C, Huang PS, Ravichandran R, Yi S, Davis TN, Gonen T, King NP, Baker D.

Nature. 2016 Jul 7;535(7610):136-9. doi: 10.1038/nature18010. Epub 2016 Jun 15. Erratum in: Nature. 2016 Dec 1;540(7631):150.


Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM.

Heinze K, Sasaki E, King NP, Baker D, Hilvert D, Wuite GJ, Roos WH.

J Phys Chem B. 2016 Jul 7;120(26):5945-52. doi: 10.1021/acs.jpcb.6b01464. Epub 2016 May 17.


Discovery and Characterization of Human-Urine Utilization by Asymptomatic-Bacteriuria-Causing Streptococcus agalactiae.

Ipe DS, Ben Zakour NL, Sullivan MJ, Beatson SA, Ulett KB, Benjamin WH Jr, Davies MR, Dando SJ, King NP, Cripps AW, Schembri MA, Dougan G, Ulett GC.

Infect Immun. 2015 Nov 9;84(1):307-19. doi: 10.1128/IAI.00938-15.


Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.

Bale JB, Park RU, Liu Y, Gonen S, Gonen T, Cascio D, King NP, Yeates TO, Baker D.

Protein Sci. 2015 Oct;24(10):1695-701. doi: 10.1002/pro.2748. Epub 2015 Aug 6.


Soluble NSF attachment protein receptor molecular mimicry by a Legionella pneumophila Dot/Icm effector.

King NP, Newton P, Schuelein R, Brown DL, Petru M, Zarsky V, Dolezal P, Luo L, Bugarcic A, Stanley AC, Murray RZ, Collins BM, Teasdale RD, Hartland EL, Stow JL.

Cell Microbiol. 2015 Jun;17(6):767-84. doi: 10.1111/cmi.12405. Epub 2015 Jan 24.


Accurate design of co-assembling multi-component protein nanomaterials.

King NP, Bale JB, Sheffler W, McNamara DE, Gonen S, Gonen T, Yeates TO, Baker D.

Nature. 2014 Jun 5;510(7503):103-8. doi: 10.1038/nature13404. Epub 2014 May 25.


Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry.

Luo L, King NP, Yeo JC, Jones A, Stow JL.

Proteomics. 2014 Jan;14(1):19-23. doi: 10.1002/pmic.201300315.


Practical approaches to designing novel protein assemblies.

King NP, Lai YT.

Curr Opin Struct Biol. 2013 Aug;23(4):632-8. doi: 10.1016/ Epub 2013 Jul 1. Review.


Structural and functional characterization of ScsC, a periplasmic thioredoxin-like protein from Salmonella enterica serovar Typhimurium.

Shepherd M, Heras B, Achard ME, King GJ, Argente MP, Kurth F, Taylor SL, Howard MJ, King NP, Schembri MA, McEwan AG.

Antioxid Redox Signal. 2013 Nov 1;19(13):1494-506. doi: 10.1089/ars.2012.4939. Epub 2013 Aug 9.


Principles for designing ordered protein assemblies.

Lai YT, King NP, Yeates TO.

Trends Cell Biol. 2012 Dec;22(12):653-61. doi: 10.1016/j.tcb.2012.08.004. Epub 2012 Sep 10. Review.


Computational design of self-assembling protein nanomaterials with atomic level accuracy.

King NP, Sheffler W, Sawaya MR, Vollmar BS, Sumida JP, André I, Gonen T, Yeates TO, Baker D.

Science. 2012 Jun 1;336(6085):1171-4. doi: 10.1126/science.1219364.


Molecular characterization of the EhaG and UpaG trimeric autotransporter proteins from pathogenic Escherichia coli.

Totsika M, Wells TJ, Beloin C, Valle J, Allsopp LP, King NP, Ghigo JM, Schembri MA.

Appl Environ Microbiol. 2012 Apr;78(7):2179-89. doi: 10.1128/AEM.06680-11. Epub 2012 Jan 27.


Characterisation of a cell wall-anchored protein of Staphylococcus saprophyticus associated with linoleic acid resistance.

King NP, Sakinç T, Ben Zakour NL, Totsika M, Heras B, Simerska P, Shepherd M, Gatermann SG, Beatson SA, Schembri MA.

BMC Microbiol. 2012 Jan 15;12:8. doi: 10.1186/1471-2180-12-8.


Protein stabilization in a highly knotted protein polymer.

Sayre TC, Lee TM, King NP, Yeates TO.

Protein Eng Des Sel. 2011 Aug;24(8):627-30. doi: 10.1093/protein/gzr024. Epub 2011 Jun 13.

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