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Items: 1 to 20 of 129


Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface.

Limsakul P, Peng Q, Wu Y, Allen ME, Liang J, Remacle AG, Lopez T, Ge X, Kay BK, Zhao H, Strongin AY, Yang XL, Lu S, Wang Y.

Cell Chem Biol. 2018 Apr 19;25(4):370-379.e4. doi: 10.1016/j.chembiol.2018.01.002. Epub 2018 Jan 27.


Generation of recombinant affinity reagents against a two-phosphosite epitope of ATF2.

McGinnis J, Kay BK.

N Biotechnol. 2017 Oct 26. pii: S1871-6784(17)30450-8. doi: 10.1016/j.nbt.2017.10.004. [Epub ahead of print]


Tandem phage-display for the identification of non-overlapping binding pairs of recombinant affinity reagents.

Gorman KT, Roby LC, Giuffre A, Huang R, Kay BK.

Nucleic Acids Res. 2017 Oct 13;45(18):e158. doi: 10.1093/nar/gkx688.


Targeted Delivery of Deoxycytidine Kinase to Her2-Positive Cells Enhances the Efficacy of the Nucleoside Analog Fludarabine.

Koduvayur SP, Su Y, Kay BK, Lavie A.

PLoS One. 2016 Jun 9;11(6):e0157114. doi: 10.1371/journal.pone.0157114. eCollection 2016.


pMINERVA: A donor-acceptor system for the in vivo recombineering of scFv into IgG molecules.

Batonick M, Kiss MM, Fuller EP, Magadan CM, Holland EG, Zhao Q, Wang D, Kay BK, Weiner MP.

J Immunol Methods. 2016 Apr;431:22-30. doi: 10.1016/j.jim.2016.02.003. Epub 2016 Feb 3.


A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides.

Venegas LA, Pershad K, Bankole O, Shah N, Kay BK.

N Biotechnol. 2016 Sep 25;33(5 Pt A):537-43. doi: 10.1016/j.nbt.2015.12.006. Epub 2016 Jan 6.


Directed Evolution of a Highly Specific FN3 Monobody to the SH3 Domain of Human Lyn Tyrosine Kinase.

Huang R, Fang P, Hao Z, Kay BK.

PLoS One. 2016 Jan 5;11(1):e0145872. doi: 10.1371/journal.pone.0145872. eCollection 2016.


Platform for high-throughput antibody selection using synthetically-designed antibody libraries.

Batonick M, Holland EG, Busygina V, Alderman D, Kay BK, Weiner MP, Kiss MM.

N Biotechnol. 2016 Sep 25;33(5 Pt A):565-73. doi: 10.1016/j.nbt.2015.11.005. Epub 2015 Nov 24.


Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step.

Huang R, Gorman KT, Vinci CR, Dobrovetsky E, Gräslund S, Kay BK.

Int J Mol Sci. 2015 Sep 30;16(10):23587-603. doi: 10.3390/ijms161023587.


Generating Recombinant Antibodies against Putative Biomarkers of Retinal Injury.

Kierny MR, Cunningham TD, Bouhenni RA, Edward DP, Kay BK.

PLoS One. 2015 Apr 22;10(4):e0124492. doi: 10.1371/journal.pone.0124492. eCollection 2015.


Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries and alanine scanning.

Kokoszka ME, Kay BK.

Methods Mol Biol. 2015;1248:173-88. doi: 10.1007/978-1-4939-2020-4_12. Review.


In vivo elimination of parental clones in general and site-directed mutagenesis.

Holland EG, Acca FE, Belanger KM, Bylo ME, Kay BK, Weiner MP, Kiss MM.

J Immunol Methods. 2015 Feb;417:67-75. doi: 10.1016/j.jim.2014.12.008. Epub 2014 Dec 15.


Generation of recombinant antibodies to rat GABAA receptor subunits by affinity selection on synthetic peptides.

Koduvayur SP, Gussin HA, Parthasarathy R, Hao Z, Kay BK, Pepperberg DR.

PLoS One. 2014 Feb 19;9(2):e87964. doi: 10.1371/journal.pone.0087964. eCollection 2014.


AXM mutagenesis: an efficient means for the production of libraries for directed evolution of proteins.

Holland EG, Buhr DL, Acca FE, Alderman D, Bovat K, Busygina V, Kay BK, Weiner MP, Kiss MM.

J Immunol Methods. 2013 Aug 30;394(1-2):55-61. doi: 10.1016/j.jim.2013.05.003. Epub 2013 May 13.


Single chain variable fragment antibodies block aggregation and toxicity induced by familial ALS-linked mutant forms of SOD1.

Ghadge GD, Pavlovic JD, Koduvayur SP, Kay BK, Roos RP.

Neurobiol Dis. 2013 Aug;56:74-8. doi: 10.1016/j.nbd.2013.04.007. Epub 2013 Apr 20. Erratum in: Neurobiol Dis. 2013 Oct;58:115.


Generating thermal stable variants of protein domains through phage display.

Pershad K, Kay BK.

Methods. 2013 Mar 15;60(1):38-45. doi: 10.1016/j.ymeth.2012.12.009. Epub 2012 Dec 29.


Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage display.

Pershad K, Wypisniak K, Kay BK.

J Mol Biol. 2012 Nov 23;424(1-2):88-103. doi: 10.1016/j.jmb.2012.09.006. Epub 2012 Sep 15.


Improvements to the Kunkel mutagenesis protocol for constructing primary and secondary phage-display libraries.

Huang R, Fang P, Kay BK.

Methods. 2012 Sep;58(1):10-7. doi: 10.1016/j.ymeth.2012.08.008. Epub 2012 Aug 30.


Detection of biomarkers using recombinant antibodies coupled to nanostructured platforms.

Kierny MR, Cunningham TD, Kay BK.

Nano Rev. 2012;3. doi: 10.3402/nano.v3i0.17240. Epub 2012 Jul 23.


Use of micro-emulsion technology for the directed evolution of antibodies.

Buhr DL, Acca FE, Holland EG, Johnson K, Maksymiuk GM, Vaill A, Kay BK, Weitz DA, Weiner MP, Kiss MM.

Methods. 2012 Sep;58(1):28-33. doi: 10.1016/j.ymeth.2012.07.007. Epub 2012 Jul 20.


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