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Items: 1 to 20 of 44

1.

A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery.

Rodrigues TA, Francisco T, Dias AF, Pedrosa AG, Grou CP, Azevedo JE.

Nat Protoc. 2016 Dec;11(12):2454-2469. doi: 10.1038/nprot.2016.147.

PMID:
27831570
2.

Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases.

Mendes AV, Grou CP, Azevedo JE, Pinto MP.

Biochim Biophys Acta. 2016 Jan;1863(1):139-47. doi: 10.1016/j.bbamcr.2015.10.020.

3.

The first minutes in the life of a peroxisomal matrix protein.

Dias AF, Francisco T, Rodrigues TA, Grou CP, Azevedo JE.

Biochim Biophys Acta. 2016 May;1863(5):814-20. doi: 10.1016/j.bbamcr.2015.09.025. Review.

PMID:
26408939
4.

The de novo synthesis of ubiquitin: identification of deubiquitinases acting on ubiquitin precursors.

Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE.

Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.

5.

Revisiting the intraperoxisomal pathway of mammalian PEX7.

Rodrigues TA, Grou CP, Azevedo JE.

Sci Rep. 2015 Jul 3;5:11806. doi: 10.1038/srep11806.

6.

Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.

Nordgren M, Francisco T, Lismont C, Hennebel L, Brees C, Wang B, Van Veldhoven PP, Azevedo JE, Fransen M.

Autophagy. 2015;11(8):1326-40. doi: 10.1080/15548627.2015.1061846.

7.

The peroxisomal protein import machinery displays a preference for monomeric substrates.

Freitas MO, Francisco T, Rodrigues TA, Lismont C, Domingues P, Pinto MP, Grou CP, Fransen M, Azevedo JE.

Open Biol. 2015 Apr;5(4):140236. doi: 10.1098/rsob.140236.

8.

Intracellular trafficking of AIP56, an NF-κB-cleaving toxin from Photobacterium damselae subsp. piscicida.

Pereira LM, Pinto RD, Silva DS, Moreira AR, Beitzinger C, Oliveira P, Sampaio P, Benz R, Azevedo JE, dos Santos NM, do Vale A.

Infect Immun. 2014 Dec;82(12):5270-85. doi: 10.1128/IAI.02623-14.

9.

A PEX7-centered perspective on the peroxisomal targeting signal type 2-mediated protein import pathway.

Rodrigues TA, Alencastre IS, Francisco T, Brites P, Fransen M, Grou CP, Azevedo JE.

Mol Cell Biol. 2014 Aug;34(15):2917-28. doi: 10.1128/MCB.01727-13.

10.

PEX5, the shuttling import receptor for peroxisomal matrix proteins, is a redox-sensitive protein.

Apanasets O, Grou CP, Van Veldhoven PP, Brees C, Wang B, Nordgren M, Dodt G, Azevedo JE, Fransen M.

Traffic. 2014 Jan;15(1):94-103. doi: 10.1111/tra.12129.

11.

A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway.

Francisco T, Rodrigues TA, Freitas MO, Grou CP, Carvalho AF, Sá-Miranda C, Pinto MP, Azevedo JE.

J Biol Chem. 2013 Oct 4;288(40):29151-9. doi: 10.1074/jbc.M113.487140.

12.

Ubiquitin in the peroxisomal protein import pathway.

Francisco T, Rodrigues TA, Pinto MP, Carvalho AF, Azevedo JE, Grou CP.

Biochimie. 2014 Mar;98:29-35. doi: 10.1016/j.biochi.2013.08.003. Review.

PMID:
23954799
13.

The apoptogenic toxin AIP56 is a metalloprotease A-B toxin that cleaves NF-κb P65.

Silva DS, Pereira LM, Moreira AR, Ferreira-da-Silva F, Brito RM, Faria TQ, Zornetta I, Montecucco C, Oliveira P, Azevedo JE, Pereira PJ, Macedo-Ribeiro S, do Vale A, dos Santos NM.

PLoS Pathog. 2013 Feb;9(2):e1003128. doi: 10.1371/journal.ppat.1003128.

14.

Caspase-1 and IL-1β processing in a teleost fish.

Reis MI, do Vale A, Pereira PJ, Azevedo JE, Dos Santos NM.

PLoS One. 2012;7(11):e50450. doi: 10.1371/journal.pone.0050450.

15.

Heat shock induces a massive but differential inactivation of SUMO-specific proteases.

Pinto MP, Carvalho AF, Grou CP, Rodríguez-Borges JE, Sá-Miranda C, Azevedo JE.

Biochim Biophys Acta. 2012 Oct;1823(10):1958-66. doi: 10.1016/j.bbamcr.2012.07.010.

16.

Identification of ubiquitin-specific protease 9X (USP9X) as a deubiquitinase acting on ubiquitin-peroxin 5 (PEX5) thioester conjugate.

Grou CP, Francisco T, Rodrigues TA, Freitas MO, Pinto MP, Carvalho AF, Domingues P, Wood SA, Rodríguez-Borges JE, Sá-Miranda C, Fransen M, Azevedo JE.

J Biol Chem. 2012 Apr 13;287(16):12815-27. doi: 10.1074/jbc.M112.340158.

17.

High-yield expression in Escherichia coli and purification of mouse ubiquitin-activating enzyme E1.

Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sá-Miranda C, Azevedo JE.

Mol Biotechnol. 2012 Jul;51(3):254-61. doi: 10.1007/s12033-011-9463-x.

PMID:
22012022
18.

PEX5 protein binds monomeric catalase blocking its tetramerization and releases it upon binding the N-terminal domain of PEX14.

Freitas MO, Francisco T, Rodrigues TA, Alencastre IS, Pinto MP, Grou CP, Carvalho AF, Fransen M, Sá-Miranda C, Azevedo JE.

J Biol Chem. 2011 Nov 25;286(47):40509-19. doi: 10.1074/jbc.M111.287201.

19.

The cytosolic domain of PEX3, a protein involved in the biogenesis of peroxisomes, binds membrane lipids.

Pinto MP, Grou CP, Fransen M, Sá-Miranda C, Azevedo JE.

Biochim Biophys Acta. 2009 Nov;1793(11):1669-75. doi: 10.1016/j.bbamcr.2009.08.007.

20.

Mapping the cargo protein membrane translocation step into the PEX5 cycling pathway.

Alencastre IS, Rodrigues TA, Grou CP, Fransen M, Sá-Miranda C, Azevedo JE.

J Biol Chem. 2009 Oct 2;284(40):27243-51. doi: 10.1074/jbc.M109.032565.

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