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Items: 1 to 20 of 51

1.

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR.

Busby E, Whale AS, Ferns RB, Grant PR, Morley G, Campbell J, Foy CA, Nastouli E, Huggett JF, Garson JA.

Sci Rep. 2017 Apr 26;7(1):1209. doi: 10.1038/s41598-017-01221-5.

2.

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA.

Pavšič J, Devonshire A, Blejec A, Foy CA, Van Heuverswyn F, Jones GM, Schimmel H, Žel J, Huggett JF, Redshaw N, Karczmarczyk M, Mozioğlu E, Akyürek S, Akgöz M, Milavec M.

Anal Bioanal Chem. 2017 Apr;409(10):2601-2614. doi: 10.1007/s00216-017-0206-0. Epub 2017 Jan 26.

3.

International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant.

Whale AS, Devonshire AS, Karlin-Neumann G, Regan J, Javier L, Cowen S, Fernandez-Gonzalez A, Jones GM, Redshaw N, Beck J, Berger AW, Combaret V, Dahl Kjersgaard N, Davis L, Fina F, Forshew T, Fredslund Andersen R, Galbiati S, González Hernández Á, Haynes CA, Janku F, Lacave R, Lee J, Mistry V, Pender A, Pradines A, Proudhon C, Saal LH, Stieglitz E, Ulrich B, Foy CA, Parkes H, Tzonev S, Huggett JF.

Anal Chem. 2017 Feb 7;89(3):1724-1733. doi: 10.1021/acs.analchem.6b03980. Epub 2017 Jan 18.

PMID:
27935690
4.

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis.

Devonshire AS, O'Sullivan DM, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo DM, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis CL, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy CA, McHugh TD, Huggett JF.

BMC Infect Dis. 2016 Aug 3;16:366. doi: 10.1186/s12879-016-1696-7.

5.

An international comparability study on quantification of mRNA gene expression ratios: CCQM-P103.1.

Devonshire AS, Sanders R, Whale AS, Nixon GJ, Cowen S, Ellison SL, Parkes H, Pine PS, Salit M, McDaniel J, Munro S, Lund S, Matsukura S, Sekiguchi Y, Kawaharasaki M, Granjeiro JM, Falagan-Lotsch P, Saraiva AM, Couto P, Yang I, Kwon H, Park SR, Demšar T, Žel J, Blejec A, Milavec M, Dong L, Zhang L, Sui Z, Wang J, Viroonudomphol D, Prawettongsopon C, Partis L, Baoutina A, Emslie K, Takatsu A, Akyurek S, Akgoz M, Vonsky M, Konopelko LA, Cundapi EM, Urquiza MP, Huggett JF, Foy CA.

Biomol Detect Quantif. 2016 Jun 6;8:15-28. doi: 10.1016/j.bdq.2016.05.003. eCollection 2016 Jun.

6.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Whale AS, Bushell CA, Grant PR, Cowen S, Gutierrez-Aguirre I, O'Sullivan DM, Žel J, Milavec M, Foy CA, Nastouli E, Garson JA, Huggett JF.

J Clin Microbiol. 2016 Feb;54(2):392-400. doi: 10.1128/JCM.02611-15. Epub 2015 Dec 9.

7.

Highly reproducible absolute quantification of Mycobacterium tuberculosis complex by digital PCR.

Devonshire AS, Honeyborne I, Gutteridge A, Whale AS, Nixon G, Wilson P, Jones G, McHugh TD, Foy CA, Huggett JF.

Anal Chem. 2015 Apr 7;87(7):3706-13. doi: 10.1021/ac5041617. Epub 2015 Mar 10.

PMID:
25646934
8.

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis.

Redshaw N, Huggett JF, Taylor MS, Foy CA, Devonshire AS.

BMC Genomics. 2014 Dec 23;15:1174. doi: 10.1186/1471-2164-15-1174.

9.

Assessing the accuracy of quantitative molecular microbial profiling.

O'Sullivan DM, Laver T, Temisak S, Redshaw N, Harris KA, Foy CA, Studholme DJ, Huggett JF.

Int J Mol Sci. 2014 Nov 21;15(11):21476-91. doi: 10.3390/ijms151121476.

10.

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.

Nixon GJ, Svenstrup HF, Donald CE, Carder C, Stephenson JM, Morris-Jones S, Huggett JF, Foy CA.

Biomol Detect Quantif. 2014 Nov 22;2:4-10. eCollection 2014 Dec.

11.

Standardization of Nucleic Acid Tests for Clinical Measurements of Bacteria and Viruses.

Pavšič J, Devonshire AS, Parkes H, Schimmel H, Foy CA, Karczmarczyk M, Gutiérrez-Aguirre I, Honeyborne I, Huggett JF, McHugh TD, Milavec M, Zeichhardt H, Žel J.

J Clin Microbiol. 2015 Jul;53(7):2008-14. doi: 10.1128/JCM.02136-14. Epub 2014 Nov 12. Review.

12.

Considerations for digital PCR as an accurate molecular diagnostic tool.

Huggett JF, Cowen S, Foy CA.

Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22. Review.

13.

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples.

Sanders R, Mason DJ, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6471-83. doi: 10.1007/s00216-014-7857-x. Epub 2014 May 25. Review.

14.

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification.

Devonshire AS, Whale AS, Gutteridge A, Jones G, Cowen S, Foy CA, Huggett JF.

Anal Bioanal Chem. 2014 Oct;406(26):6499-512. doi: 10.1007/s00216-014-7835-3. Epub 2014 May 24.

15.
16.

Validation of high-throughput single cell analysis methodology.

Devonshire AS, Baradez MO, Morley G, Marshall D, Foy CA.

Anal Biochem. 2014 May 1;452:103-13. doi: 10.1016/j.ab.2014.03.001. Epub 2014 Mar 11.

PMID:
24631519
17.

Long-term outcome following carpal wedge osteotomy in the arthrogrypotic patient.

Foy CA, Mills J, Wheeler L, Ezaki M, Oishi SN.

J Bone Joint Surg Am. 2013 Oct 16;95(20):e150. doi: 10.2106/JBJS.L.01122.

PMID:
24132365
18.

Evaluation of digital PCR for absolute RNA quantification.

Sanders R, Mason DJ, Foy CA, Huggett JF.

PLoS One. 2013 Sep 20;8(9):e75296. doi: 10.1371/journal.pone.0075296. eCollection 2013.

19.

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.

Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA.

Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9.

20.

A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability.

Redshaw N, Wilkes T, Whale A, Cowen S, Huggett J, Foy CA.

Biotechniques. 2013 Mar;54(3):155-64. doi: 10.2144/000114002.

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