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Items: 1 to 20 of 48


Site-Specific Incorporation of a Cu2+ Spin Label into Proteins for Measuring Distances by Pulsed Dipolar Electron Spin Resonance Spectroscopy.

Merz GE, Borbat PP, Muok AR, Srivastava M, Bunck DN, Freed JH, Crane BR.

J Phys Chem B. 2018 Oct 18;122(41):9443-9451. doi: 10.1021/acs.jpcb.8b05619. Epub 2018 Oct 3.


Open and Closed Form of Maltose Binding Protein in Its Native and Molten Globule State As Studied by Electron Paramagnetic Resonance Spectroscopy.

Selmke B, Borbat PP, Nickolaus C, Varadarajan R, Freed JH, Trommer WE.

Biochemistry. 2018 Sep 25;57(38):5507-5512. doi: 10.1021/acs.biochem.8b00322. Epub 2018 Sep 13.


A facile approach for the in vitro assembly of multimeric membrane transport proteins.

Riederer EA, Focke PJ, Georgieva ER, Akyuz N, Matulef K, Borbat PP, Freed JH, Blanchard SC, Boudker O, Valiyaveetil FI.

Elife. 2018 Jun 11;7. pii: e36478. doi: 10.7554/eLife.36478.


Structural basis for membrane anchoring and fusion regulation of the herpes simplex virus fusogen gB.

Cooper RS, Georgieva ER, Borbat PP, Freed JH, Heldwein EE.

Nat Struct Mol Biol. 2018 May;25(5):416-424. doi: 10.1038/s41594-018-0060-6. Epub 2018 May 4.


Key features of an Hsp70 chaperone allosteric landscape revealed by ion-mobility native mass spectrometry and double electron-electron resonance.

Lai AL, Clerico EM, Blackburn ME, Patel NA, Robinson CV, Borbat PP, Freed JH, Gierasch LM.

J Biol Chem. 2017 May 26;292(21):8773-8785. doi: 10.1074/jbc.M116.770404. Epub 2017 Apr 20.


Stability and Conformation of a Chemoreceptor HAMP Domain Chimera Correlates with Signaling Properties.

Sukomon N, Widom J, Borbat PP, Freed JH, Crane BR.

Biophys J. 2017 Apr 11;112(7):1383-1395. doi: 10.1016/j.bpj.2017.02.037.


Signature of an aggregation-prone conformation of tau.

Eschmann NA, Georgieva ER, Ganguly P, Borbat PP, Rappaport MD, Akdogan Y, Freed JH, Shea JE, Han S.

Sci Rep. 2017 Mar 17;7:44739. doi: 10.1038/srep44739.


Bacterial Energy Sensor Aer Modulates the Activity of the Chemotaxis Kinase CheA Based on the Redox State of the Flavin Cofactor.

Samanta D, Widom J, Borbat PP, Freed JH, Crane BR.

J Biol Chem. 2016 Dec 9;291(50):25809-25814. Epub 2016 Nov 1.


Conformational Response of Influenza A M2 Transmembrane Domain to Amantadine Drug Binding at Low pH (pH 5.5).

Georgieva ER, Borbat PP, Grushin K, Stoilova-McPhie S, Kulkarni NJ, Liang Z, Freed JH.

Front Physiol. 2016 Jul 29;7:317. doi: 10.3389/fphys.2016.00317. eCollection 2016.


Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.

Tang S, Henne WM, Borbat PP, Buchkovich NJ, Freed JH, Mao Y, Fromme JC, Emr SD.

Elife. 2015 Dec 15;4. pii: e12548. doi: 10.7554/eLife.12548.


Signal transduction in light-oxygen-voltage receptors lacking the adduct-forming cysteine residue.

Yee EF, Diensthuber RP, Vaidya AT, Borbat PP, Engelhard C, Freed JH, Bittl R, Möglich A, Crane BR.

Nat Commun. 2015 Dec 9;6:10079. doi: 10.1038/ncomms10079.


Pulsed Dipolar Spectroscopy Reveals That Tyrosyl Radicals Are Generated in Both Monomers of the Cyclooxygenase-2 Dimer.

Orlando BJ, Borbat PP, Georgieva ER, Freed JH, Malkowski MG.

Biochemistry. 2015 Dec 22;54(50):7309-12. doi: 10.1021/acs.biochem.5b00979. Epub 2015 Dec 7.


Mechanism of influenza A M2 transmembrane domain assembly in lipid membranes.

Georgieva ER, Borbat PP, Norman HD, Freed JH.

Sci Rep. 2015 Jul 20;5:11757. doi: 10.1038/srep11757.


Preformed Soluble Chemoreceptor Trimers That Mimic Cellular Assembly States and Activate CheA Autophosphorylation.

Greenswag AR, Li X, Borbat PP, Samanta D, Watts KJ, Freed JH, Crane BR.

Biochemistry. 2015 Jun 9;54(22):3454-68. doi: 10.1021/bi501570n. Epub 2015 May 28.


Pulse dipolar ESR of doubly labeled mini TAR DNA and its annealing to mini TAR RNA.

Sun Y, Borbat PP, Grigoryants VM, Myers WK, Freed JH, Scholes CP.

Biophys J. 2015 Feb 17;108(4):893-902. doi: 10.1016/j.bpj.2014.12.028.


Bacterial chemoreceptor dynamics correlate with activity state and are coupled over long distances.

Samanta D, Borbat PP, Dzikovski B, Freed JH, Crane BR.

Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2455-60. doi: 10.1073/pnas.1414155112. Epub 2015 Feb 9.


Assembly states of FliM and FliG within the flagellar switch complex.

Sircar R, Borbat PP, Lynch MJ, Bhatnagar J, Beyersdorf MS, Halkides CJ, Freed JH, Crane BR.

J Mol Biol. 2015 Feb 27;427(4):867-886. doi: 10.1016/j.jmb.2014.12.009. Epub 2014 Dec 20.


Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes.

Pratt AJ, Shin DS, Merz GE, Rambo RP, Lancaster WA, Dyer KN, Borbat PP, Poole FL 2nd, Adams MW, Freed JH, Crane BR, Tainer JA, Getzoff ED.

Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4568-76. doi: 10.1073/pnas.1308531111. Epub 2014 Oct 14.


Copper-based pulsed dipolar ESR spectroscopy as a probe of protein conformation linked to disease states.

Merz GE, Borbat PP, Pratt AJ, Getzoff ED, Freed JH, Crane BR.

Biophys J. 2014 Oct 7;107(7):1669-74. doi: 10.1016/j.bpj.2014.07.068.


Tau binds to lipid membrane surfaces via short amphipathic helices located in its microtubule-binding repeats.

Georgieva ER, Xiao S, Borbat PP, Freed JH, Eliezer D.

Biophys J. 2014 Sep 16;107(6):1441-52. doi: 10.1016/j.bpj.2014.07.046.

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