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1.
Int J Biol Macromol. 2018 May;111:393-399. doi: 10.1016/j.ijbiomac.2018.01.014. Epub 2018 Jan 5.

Microwave functionalization of titanium oxide nanoparticles with chitosan nanolayer for instantaneous microwave sorption of Cu(II) and Cd(II) from water.

Author information

1
Faculty of Sciences, Chemistry Department, Alexandria University, P.O. Box 426, Ibrahimia, 21321 Alexandria, Egypt. Electronic address: memahmoud10@alexu.edu.eg.
2
Faculty of Education, Chemistry and Physics Department, Alexandria University, El-Shatby, Alexandria, Egypt.

Abstract

The present study is aimed to evaluate the microwave-enforced sorption approach (MES) for instantaneous extraction and removal of trace concentration of metal ions using microwave-synthesized titanium oxide nanoparticles-bonded-chitosan nanolayer (NTiO2-NCh). The proposed and designed nanocomposite was characterized by different techniques. The coveted ions were allowed to heat in presence of NTiO2-NCh nanocomposite inside a microwave apparatus for 5-20s to execute the sorption process. The contribution of microwave warming time, nanocomposite dose, concentration of Cd(II) and Cu(II) ions, pH and interfering ions were explored and optimized. Sorption of Cd(II) was characterized as 1050 and 1150μmolg-1 and those of Cu(II) were identified as 450 and 800μmolg-1 using 5 and 20s of microwave heating time, respectively. Optimization of the nanocomposite dose factor was found to enhance the metal uptake values of Cd(II) to 1800μmolg-1 using 5.0mg. The potential utilization of MES technique for removal and extraction of Cd(II) and Cu(II) at low concentration levels (mgL-1) from water samples was also explored. The percentage extraction values of Cu(II) and Cd(II) from water and wastewater samples were ranged as 86.80-88.01% and 72.56-70.67%, respectively using 60-70s heating via MES technique.

KEYWORDS:

Chitosan nanolayer; Covalent bonding; Heavy metals; MES technique; Sorption; Titanium oxide nanoparticles

2.
Int J Biol Macromol. 2017 Aug;101:230-240. doi: 10.1016/j.ijbiomac.2017.03.049. Epub 2017 Mar 11.

Immobilization of chitosan nanolayers on the surface of nano-titanium oxide as a novel nanocomposite for efficient removal of La(III) from water.

Author information

1
Faculty of Sciences, Chemistry Department, Alexandria University, P.O. Box 426, Ibrahimia, 21321, Alexandria, Egypt. Electronic address: memahmoud10@yahoo.com.
2
Faculty of Education, Chemistry and Physics Department, Alexandria University, El-Shatby, Alexandria, Egypt.
3
Faculty of Sciences, Chemistry Department, Alexandria University, P.O. Box 426, Ibrahimia, 21321, Alexandria, Egypt.
4
National Institute of Oceanography and Fisheries (NIOF), Marine Biotechnology and Natural Products Extraction, Alexandria, Egypt.

Abstract

A novel nanocomposite has been designed and synthesized via surface crosslinking of chitosan nanolayers (NChit) with titanium oxide nanoparticles (NTiO2) using glutaraldehyde (Glu) as the crosslinking agent. A simple and green surface chemical reaction was accomplished by the aid of microwave heating process to enforce surface encapsulation and functionalization for the production of the aimed NTiO2-Glu-NChit nanocomposite. The average particles size of nanocomposite was characterized in the range of 52-58nm using SEM and confirmed by the HR-TEM. The XRD, TGA and FTIR were also employed to assure the immobilization and crosslinking processes. NTiO2-Glu-NChit was studied to estimate the sorption efficiency towards La(III) from aqueous solution by the batch technique under different experimental controlling physicochemical parameters such as, initial pH of metal ion solution, contact time, nanocomposite dosage and initial metal ion concentration. The optimum sorption condition for La(III) as the target metal ion was identified at pH=1.0, 3.0 and 6.0. The adsorption process of La(III) was characterized to follow the postulates of Langmuir and Freundlich isotherm models and the adsorption mechanisms were identified to obey the pseudo-second order kinetic model based on the best compatible results with the experimental data.

KEYWORDS:

Chitosan; Crosslinking; Lanthanum (III); Nano-titanium oxide; Nanocomposite

PMID:
28300588
DOI:
10.1016/j.ijbiomac.2017.03.049
[Indexed for MEDLINE]
Icon for Elsevier Science
3.
Clin Exp Hypertens. 2016;38(4):370-4. doi: 10.3109/10641963.2015.1131286. Epub 2016 May 5.

Postmenopausal hypertension, abdominal obesity, apolipoprotein and insulin resistance.

Author information

1
a Laboratory of Epidemiology and Prevention of Cardiovascular Disease , Faculty of Medicine , Tunis , Tunisia.
2
b Center of Basic Health Care of Ariana Essoghra , Tunis , Tunisia.
3
c Laboratory of Medical Biology, Mahmoud El-Matri Hospital of Ariana , Tunis , Tunisia.

Abstract

This study aimed to evaluate the association of abdominal obesity, apolipoprotein and insulin resistance (IR) with the risk of hypertension in postmenopausal women. We analyzed a total of 242 women aged between 35 and 70 years. Blood pressure (BP), anthropometric indices, lipid profile, fasting glucose, insulin, C-reactive protein (CRP) and apolipoprotein concentrations were measured. Homeostasis model assessment (HOMA) was used to assess IR. Hypertension was defined as a systolic BP (SBP) ≥140 mmHg and/or diastolic BP (DBP) ≥90 mmHg or current treatment with antihypertensive drugs. Women with hypertension showed significantly higher mean values of age, SBP and DBP, waist circumference (WC), fasting plasma glucose (FPG), insulin, HOMAIR and the apolipoprotein B (apoB). When analyses were done according to the menopausal status, higher prevalence of hypertension was observed in postmenopausal women (72.8% vs. 26.0%, p < 0.001) compared to their premenopausal counterparts. Postmenopausal women showed also significantly higher mean values of SBP and DBP, WC, HOMAIR and apoB. Multivariate linear regression analysis revealed that SBP was significantly affected by WC (p = 0.034), apoB (p = 0.038) and log HOMAIR (p = 0.007) in postmenopausal women. The interaction models revealed significant interaction between WC, apoB and log HOMAIR (WC×apoB×log HOMAIR) on SBP (p = 0.001) adjusted for age. In a multivariate logistic regression, adjusting for age and apoB, WC (p = 0.001), log HOMAIR (p = 0.007) and menopause (p = 0.008) were significantly associated with higher risk for hypertension. These results suggest that changes in WC, apoB and IR accompanying menopause lead to a greater prevalence of hypertension in postmenopausal women.

KEYWORDS:

Abdominal obesity; apolipoprotein; hypertension; insulin resistance; menopause

PMID:
27149156
DOI:
10.3109/10641963.2015.1131286
[Indexed for MEDLINE]
Icon for Taylor & Francis
4.
Scand J Trauma Resusc Emerg Med. 2015 Jan 16;23:6. doi: 10.1186/s13049-015-0088-0.

Intestinal fatty acid binding protein as a marker for intra-abdominal pressure-related complications in patients admitted to the intensive care unit; study protocol for a prospective cohort study (I-Fabulous study).

Abstract

BACKGROUND:

Intra-abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) have detrimental effects on all organ systems and are associated with increased morbidity and mortality in critically ill patients admitted to an intensive care unit. Intra-bladder measurement of the intra-abdominal pressure (IAP) is currently the gold standard. However, IAH is not always indicative of intestinal ischemia, which is an early and rapidly developing complication. Sensitive biomarkers for intestinal ischemia are needed to be able to intervene before damage becomes irreversible. Gut wall integrity loss, including epithelial cell disruption and tight junctions breakdown, is an early event in intestinal damage. Intestinal Fatty Acid Binding Protein (I-FABP) is excreted in urine and blood specifically from damaged intestinal epithelial cells. Claudin-3 is a specific protein which is excreted in urine following disruption of intercellular tight junctions. This study aims to investigate if I-FABP and Claudin-3 can be used as a diagnostic tool for identifying patients at risk for IAP-related complications.

METHODS/DESIGN:

In a multicenter, prospective cohort study 200 adult patients admitted to the intensive care unit with at least two risk factors for IAH as defined by the World Society of the Abdominal Compartment Syndrome (WSACS) will be included. Patients in whom an intra-bladder IAP measurement is contra-indicated or impossible and patients with inflammatory bowel diseases that may affect I-FABP levels will be excluded. The IAP will be measured using an intra-bladder technique. During the subsequent 72 hours, the IAP measurement will be repeated every six hours. At these time points, a urine and serum sample will be collected for measurement of I-FABP and Claudin-3 levels. Clinical outcome of patients during their stay at the intensive care unit will be monitored using the Sequential Organ Failure Assessment (SOFA) score.

DISCUSSION:

Successful completion of this trial will provide evidence on the eventual role of the biomarkers I-FABP and Claudin-3 in predicting the risk of IAP-associated adverse outcome. This may aid early (surgical) intervention.

TRIAL REGISTRATION:

The trial is registered at the Netherlands Trial Register (NTR4638).

PMID:
25591785
PMCID:
PMC4324026
DOI:
10.1186/s13049-015-0088-0
[Indexed for MEDLINE]
Free PMC Article
Icon for BioMed Central Icon for PubMed Central
5.
J Tehran Heart Cent. 2014 Jan 12;9(1):20-6.

Lipid and glucose serum levels in children with congenital heart disease.

Author information

1
Emam Hosein Medical, Educational and Research Center, Esfahan University of Medical Sciences, Esfahan, Iran.
2
Golestan Hospital, Jondi Shapoor University of Medical Sciences, Ahvaz, Iran.
3
Aboozar Hospital, Jondi Shapoor University of Medical Sciences, Ahvaz, Iran.

Abstract

INTRODUCTION:

Coronary artery disease is one of the most common causes of morbidity and mortality in developed countries. Atherosclerosis begins in early childhood and progresses through life. With advances in pediatric cardiology, the prevalence of congenital heart disease in adults has increased in relation to children. A great deal of research has been conducted on serum glucose and lipid concentrations in patients with congenital heart disease, but comparison has yet to be made between congenital patients and the general population, especially in pediatric groups. The aim of this study was to compare the serum concentrations of glucose and lipids between pediatric congenital heart disease patients and a healthy age and sex-matched control group.

METHODS:

We measured and compared the total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride (TG), and plasma glucose concentrations of 100 pediatric congenital heart disease patients (cases) and 100 individuals matched for age and sex (controls) during a period of 7 months between November 2011 and June 2012.

RESULTS:

Total cholesterol, triglyceride, HDL cholesterol, and LDL cholesterol concentrations were significantly higher in the patients than in the control group (p value < 0.05). Blood sugar levels in both groups had no significant difference (p value = 0.25). In the case group, the cholesterol level was higher in the males than in the females (p value = 0.30); moreover, the TG and HDL cholesterol levels were lower in the males than in the females and the LDL cholesterol and blood sugar levels had no statistically significant difference. In the control group, there was no difference between the males and females in terms of the cholesterol, HDL cholesterol, LDL cholesterol, TG, and blood sugar levels.

CONCLUSION:

The results of this study showed that our pediatric congenital heart disease patients had significantly higher levels of serum lipids than did their age and sex-matched controls. In light of these results, we recommend that the lipid profile be screened in children with congenital heart disease so as to reduce the risk of atherosclerosis.

KEYWORDS:

Heart defects; congenital • Lipids • Glucose • Atherosclerosis

6.
Biomed Res Int. 2014;2014:457131. doi: 10.1155/2014/457131. Epub 2014 Mar 31.

Menopause and metabolic syndrome in tunisian women.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, 15 rue Djebel Akdhar, La Rabta, Bab Saâdoun, 1007 Tunis, Tunisia.
2
Institut de Recherche pour le Développement (IRD), UMR 204 NUTRIPASS, IRD-UM1-UM2, 911 Avenue Agropolis, 34394 Montpellier, France.

Abstract

OBJECTIVES:

This study aimed to evaluate the effect of menopausal status on the risk of metabolic syndrome (MetS) in Tunisian women.

METHODS:

We analyzed a total of 2680 women aged between 35 and 70 years. Blood pressure, anthropometric indices, fasting glucose, and lipid profile were measured. The MetS was assessed by the modified NCEP-ATPIII definition.

RESULTS:

The mean values of waist circumference, blood pressure, plasma lipids, and fasting glucose were significantly higher in postmenopausal than in premenopausal women, a difference that was no longer present when adjusting for age. Except for hypertriglyceridaemia, the frequency of central obesity, hyperglycemia, high blood pressure, and high total cholesterol was significantly higher in postmenopausal than in premenopausal women. After adjusting for age, the significance persisted only for hyperglycemia. The overall prevalence of MetS was 35.9%, higher in postmenopausal (45.7% versus 25.6%) than in premenopausal women. A binary logistic regression analysis showed that menopause was independently associated with MetS (OR = 1.41, 95% CI 1.10-1.82) after adjusting for age, residence area, marital status, family history of cardiovascular disease, education level, and occupation.

CONCLUSIONS:

The present study provides evidence that the MetS is highly prevalent in this group of women. Menopause can be a predictor of MetS independent of age in Tunisian women.

PMID:
24800228
PMCID:
PMC3988895
DOI:
10.1155/2014/457131
[Indexed for MEDLINE]
Free PMC Article
Icon for Hindawi Publishing Corporation Icon for PubMed Central
7.
BMC Public Health. 2014 Jan 28;14:86. doi: 10.1186/1471-2458-14-86.

Prevalence of diabetes in Northern African countries: the case of Tunisia.

Author information

1
Cardiovascular Epidemiology and Prevention Research Laboratory, Faculty of Medicine, 15 rue Djebel Akdhar-La Rabta-1007 Bab Saâdoun, Tunis, Tunisia. habibabr@yahoo.fr.

Abstract

BACKGROUND:

Although diabetes is recognized as an emerging disease in African and Middle East, few population-based surveys have been conducted in this region. We performed a national survey to estimate the prevalence of type 2 diabetes (T2D) and to evaluate the relationship between this diagnosis, demographic and socioeconomic variables.

METHODS:

The study was conducted on a random sample of 6580 households (940 in each region). 7700 subjects adults 35-70 years old were included in the analyses. T2D was assessed on the basis of a questionnaire and fasting blood glucose level according to the WHO criteria. Access to health care and diabetes management were also assessed.

RESULTS:

Overall, the prevalence of T2D was 15.1%. There were sharp urban vs. rural contrasts, the prevalence of diabetes being twice higher in urban area. However, the ratio urban/rural varied from 3 in the less developed region to 1.6 in the most developed ones. A sharp increase of prevalence of T2D with economic level of the household was observed. For both genders those with a family history of T2D were much more at risk of T2D than those without. Awareness increase with age, economic level and were higher amongst those with family history of T2D. Drugs were supplied by primary health care centers for 57.7% with a difference according to gender, 48.9% for men vs. 66.0% women (p < 0.001) and area, 53.3% on urban area vs. 75.2% on rural one (p < 0.001).

CONCLUSIONS:

Through its capacity to provide the data on the burden of diabetes in the context of the epidemiological transition that North Africa is facing, this survey will not only be valuable source for health care planners in Tunisia, but will also serve as an important research for the study of diabetes in the region where data is scarce. In this context, NCDs emerge as an intersectoral challenge and their social determinants requiring social, food and environmental health policy.

PMID:
24472619
PMCID:
PMC3933383
DOI:
10.1186/1471-2458-14-86
[Indexed for MEDLINE]
Free PMC Article
Icon for BioMed Central Icon for PubMed Central
8.
Public Health Nutr. 2013 Apr;16(4):582-90. doi: 10.1017/S1368980012003291. Epub 2012 Aug 13.

Prevalence and determinants of the metabolic syndrome among Tunisian adults: results of the Transition and Health Impact in North Africa (TAHINA) project.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, 15 rue Djebel Akdhar-La Rabta-1007 Bab Saâdoun, Tunis, Tunisia.

Abstract

OBJECTIVE:

To determine the prevalence of metabolic syndrome (MetS) and its components and to evaluate the relationship between this diagnosis and cardiovascular risk factors, demographic and socio-economic variables.

DESIGN:

A cross-sectional study using a questionnaire including information on sociodemographic and CVD risk factors. Blood pressure, anthropometric indices, fasting glucose and lipid profile were measured. MetS was defined according to the criteria of the National Cholesterol Education Program, Adult Treatment Panel III.

SETTING:

The whole Tunisian territory; Transition and Health Impact in North Africa (TAHINA) project.

SUBJECTS:

A total of 4654 individuals (1840 men and 2814 women), aged 35 to 74 years, who participated in the Tunisian national survey.

RESULTS:

The overall prevalence of MetS was 30·0 %, higher in women (36·1 %) than in men (20·6 %; P < 0·001). In both genders MetS prevalence increased significantly with age (P < 0·001), but this increase was more important in women. Multiple regression analyses showed that the odds for MetS increased significantly with urban area for both men and women (P < 0·05 and P < 0·001, respectively). The multivariate models showed also that the odds for MetS increased significantly with increasing level of education and in those with a family history of CVD for men (both P < 0·05) and after the menopausal transition for women (P < 0·05).

CONCLUSIONS:

The study highlights the MetS problem in a middle-income developing country. There is an urgent need for a comprehensive, integrated, population-based intervention programme to ameliorate the growing problem of MetS in Tunisians.

PMID:
22883486
DOI:
10.1017/S1368980012003291
[Indexed for MEDLINE]
Icon for Cambridge University Press
9.
Eur J Intern Med. 2012 Jan;23(1):e5-9. doi: 10.1016/j.ejim.2011.10.011. Epub 2011 Nov 12.

Relationship of C-reactive protein with components of the metabolic syndrome in a Tunisian population.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, Tunis, Tunisia. hanen_belfki@yahoo.fr

Abstract

BACKGROUND:

C-reactive protein (CRP) is an independent risk factor of diabetes and cardiovascular disease and it is proposed as a component of metabolic syndrome (MS). This study was undertaken to investigate the relationship between CRP and various characteristics of the MS in a sample of the Tunisian population

METHODS:

One hundred and forty nine patients with MS and 152 controls, aged 35-70 years were recruited. Waist circumference (WC), blood pressure, HDL-cholesterol (HDL-C), triglycerides (TG), glucose, insulin and CRP were measured. Insulin resistance was assessed by homeostasis model assessment of insulin resistance (HOMA-IR). MS was defined by NCEP-ATPIII report

RESULTS:

CRP levels were significantly higher in MS group (4.41±3.73 mg/L vs. 2.68±2.59 mg/L, p<0.001) compared to without MS group. For both sexes, CRP increased as the number of MS components increased (p=0.015 for men and p<0.001) after adjustment for age, smoking, alcohol intake and, for women, menopause. There were statistically significant positive correlations for log CRP with WC, log TG, and log HOMA-IR in both sexes adjusted for confounding factors listed above. A significant negative correlation was found between HDL-C and log CRP only in women. In both sexes, WC was identified, by multiple linear regression models, as significant independent predictor of CRP level variability. HDL-C showed also a significant contribution only in women

CONCLUSIONS:

The present study provides evidence that CRP levels are elevated in MS subjects. In addition, WC and HDL-C are significant predictors of the CRP elevation.

PMID:
22153549
DOI:
10.1016/j.ejim.2011.10.011
[Indexed for MEDLINE]
Icon for Elsevier Science
10.
Hypertens Res. 2012 Mar;35(3):341-7. doi: 10.1038/hr.2011.198. Epub 2011 Dec 1.

Hypertension among Tunisian adults: results of the TAHINA project.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, Tunis, Tunisia. habibabr@yahoo.fr

Abstract

We performed a national survey to determine the prevalence, awareness, treatment and control of hypertension, one of the main cardiovascular risk factors, among the adult population in Tunisia. A total of 8007 adults aged 35-70 years were included in the study. Blood pressure (BP) measurements were taken by physicians with a mercury sphygmomanometer, and standard interviewing procedures were used to record medical history, socio-demographic and cardiovascular disease (CVD) risk factors. Hypertension was defined as a systolic BP ≥140 mm Hg and/or diastolic BP ≥90 mm Hg or current treatment with antihypertensive drugs. The prevalence of hypertension was 30.6%, higher in women (33.5%) than in men (27.3%). Multiple logistic regression analyses identified a higher age, urban area, higher body mass index, type 2 diabetes and family history of CVD as important correlates to the prevalence of hypertension. Only 38.8% of those with hypertension were aware of their diagnosis, of which 84.8% were receiving treatment. BP control was achieved in only 24.1% of treated hypertensive persons. Women were more aware than men (44.8 vs. 28.8%), but the rates of treatment and control of hypertension did not differ between the two genders. Higher age, being female, lower education level and urban area emerged as important correlates of hypertension awareness. The study highlights the hypertension problem in a middle-income developing country. There is an urgent need for a comprehensive integrated population-based intervention program to ameliorate the growing problem of hypertension in Tunisians.

PMID:
22129519
DOI:
10.1038/hr.2011.198
[Indexed for MEDLINE]
Icon for Nature Publishing Group
11.
Inflammation. 2012 Jun;35(3):828-33. doi: 10.1007/s10753-011-9383-8.

Adiponectin and metabolic syndrome in a Tunisian population.

Author information

1
Research Laboratory LR99ES11, Biochemistry Department, Rabta University Hospital, Jabbari, Tunis 1007, Tunisia. Samir.BenAli@fst.rnu.tn

Abstract

The aim of this study was to investigate the relationship between the adiponectin levels and various characteristics of the metabolic syndrome (MS) in a sample of the Tunisian population. Three hundred and fifty-four individuals were included in this study. Body mass index, blood pressure, HDL-cholesterol, triglycerides, glucose, insulin, and adiponectin concentrations were measured. Insulin resistance was assessed by homeostasis model assessment of insulin resistance (HOMA-IR). MS was identified with the NCEP-ATP III criteria. Subjects with MS showed significantly lower adiponectin levels compared to those without MS. For both genders, the prevalence and the number of MS components increased significantly as the adiponectin concentrations decreased. Subjects with the lowest adiponectin quartile had an increased risk of MS adjusted for age, gender, and HOMA-IR. Our findings suggest that hypoadiponectinemia is strongly associated with the risk of MS independent of insulin resistance.

PMID:
21912966
DOI:
10.1007/s10753-011-9383-8
[Indexed for MEDLINE]
Icon for Springer
12.
Cytokine. 2011 Nov;56(2):338-42. doi: 10.1016/j.cyto.2011.06.026. Epub 2011 Jul 28.

Relationship of plasma leptin and adiponectin concentrations with menopausal status in Tunisian women.

Author information

1
Research Laboratory LR99ES11, Biochemistry Department, Rabta University Hospital, Tunis, Tunisia. Samir.BenAli@fst.rnu.tn

Abstract

To evaluate the effect of menopausal status and body mass index (BMI) on circulating leptin and adiponectin concentrations and investigate whether there is an influence of menopausal transition on the relationships of these adipokines and leptin to adiponectin (L/A) ratio with lipid profile and insulin resistance in a sample of Tunisian women. One hundred ninety-six premenopausal (mean age 35.3±7.6 years) and 180 postmenopausal women (mean age 53.4±6.2 years) were included in the study. Participants were stratified into obese and normal weight groups based upon their baseline BMI. Fasting glucose, HDL-cholesterol (HDL-C), triglycerides (TG), total cholesterol (TC), insulin, leptin, and adiponectin concentrations were measured. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. Premenopausal women had significantly higher leptin and L/A ratio and lower adiponectin levels than postmenopausal women. Menopause had no effect on the mean values of BMI, insulin or HOMA-IR, HDL-C, and TG. Using a multiple linear regression model, menopausal status was identified, as significant independent predictor for leptin and adiponectin levels. Irrespective of the menopausal status, obese women exhibited higher leptin and L/A ratio and lower adiponectin levels compared to those with normal weight. Comparison between the two menopausal stages in obese and normal weight groups showed that leptin and L/A ratio decreased, while adiponectin increased from pre- to postmenopausal stage only in obese group. The L/A ratio correlated better with lipid profile and HOMA-IR in postmenopausal stage. The present study showed a significant interaction between menopause and BMI on leptin and adiponectin secretion. Menopausal transition affects the relationships of these adipokines with lipids and insulin resistance.

PMID:
21802312
DOI:
10.1016/j.cyto.2011.06.026
[Indexed for MEDLINE]
Icon for Elsevier Science
13.
Exp Mol Pathol. 2011 Oct;91(2):622-5. doi: 10.1016/j.yexmp.2011.06.007. Epub 2011 Jul 23.

The Apolipoprotein B/Apolipoprotein A 1 ratio in relation to metabolic syndrome and its components in a sample of the Tunisian population.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, Tunis, Tunisia. hanen_belfki@yahoo.fr

Abstract

OBJECTIVE:

This study was undertaken to investigate the relationship between the Apolipoprotein B/Apolipoprotein A 1 (ApoB/ApoA 1) ratio and various characteristics of the metabolic syndrome (MetS) in a sample of the Tunisian population.

METHODS:

The study included 330 adults aged 35-74 (172 patients with MetS and 158 controls). Waist circumference (WC), blood pressure (BP), HDL-cholesterol (HDL-C), triglycerides (TG), glucose, insulin, and apolipoprotein concentrations were measured. Homeostasis model assessment (HOMA) was used to assess insulin resistance (IR). MetS was defined by NCEP-ATPIII report.

RESULTS:

The ApoB/ApoA 1 ratio was significantly higher in patients with MetS versus normal control subjects (p<0.001). Mean values of ApoB/ApoA 1 ratio increased significantly as the numbers of MetS components increased in men (p<0.001) and women (p<0.001). ApoB/ApoA 1 ratio showed statistically significant associations with WC, HDL-C, TG, systolic and diastolic BP, and HOMA-IR. After adjusting for age and gender, the high ApoB/ApoA 1 ratio was significantly associated with the presence of MetS (odds ratio [OR]=6.10), IR (OR=1.88), and with each of the MetS components, including: high WC (OR=2.43), High TG (OR=6.14), and low HDL-C (OR=6.92).

CONCLUSIONS:

Our findings suggest that the ApoB/ApoA 1 ratio is strongly associated with MetS and its components, as well as with IR.

PMID:
21801720
DOI:
10.1016/j.yexmp.2011.06.007
[Indexed for MEDLINE]
Icon for Elsevier Science
14.
Inflammation. 2012 Apr;35(2):684-9. doi: 10.1007/s10753-011-9361-1.

Association between C-reactive protein and type 2 diabetes in a Tunisian population.

Author information

1
Laboratory of Epidemiology and Prevention of Cardiovascular Disease, Faculty of Medicine, University of Tunis El-Manar, 15 rue Djebel Akdhar-La Rabta-1007 Bab Saâdoun, Tunis, Tunisia. hanen_belfki@yahoo.fr

Abstract

The aim of this study was to investigate the association of CRP levels with type 2 diabetes (T2D) and its related variables in a sample of the Tunisian population. Our sample included 129 patients with T2D and 187 control subjects. Body mass index (BMI), plasma lipids, glucose, insulin, and CRP concentrations were measured for each participant. Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated. T2D was defined as a fasting plasma glucose (FPG) level ≥ 7.0 mmol/L, the use of anti-diabetic drugs, or both. Statistical analyses were performed using SPSS 11.5. A significant difference in mean values of BMI, plasma lipids, FPG, insulin, and HOMA-IR was observed between subjects with and without T2D. CRP level was significantly higher in subjects with T2D than those without (p = 0.023), and this result persisted even after adjustment for age, gender, BMI, smoking, and alcohol consumption. In both diabetes statuses, log CRP was significantly associated with FPG, insulin, and HOMA-IR. Subjects with elevated CRP levels (>5 mg/L) had an increased risk of T2D (OR = 2.02, 95% CI 1.18-3.46, p = 0.010) than those whose CRP levels were less or equal to 5 mg/L. Even after adjustment for potentially confounding factors, the risk of T2D was still increased in subjects with elevated CRP levels (OR = 1.91, 95% CI 1.08-3.36, p = 0.025). These results suggest that elevated CRP levels are independently associated with T2D.

PMID:
21769439
DOI:
10.1007/s10753-011-9361-1
[Indexed for MEDLINE]
Icon for Springer
15.
Clin Biochem. 2010 Jan;43(1-2):106-9. doi: 10.1016/j.clinbiochem.2009.10.058. Epub 2009 Nov 5.

Association of rs2781666 G/T polymorphism of arginase I gene with myocardial infarction in Tunisian male population.

Author information

1
LR99ES11 Research Laboratory, Department of Biochemistry, Rabta Hospital, Tunis, Tunisia.

Abstract

OBJECTIVES:

The aim of this study was to investigate the association between rs2781666 G/T polymorphism of arginase I (ARG I) gene and myocardial infarction (MI) in the Tunisian male population.

DESIGN AND METHODS:

Three hundred eighteen patients with MI and 282 controls were recruited. The rs2781666 G/T polymorphism of ARG I was determined by PCR-RFLP analysis.

RESULTS:

Patients had significantly higher frequency of TT genotype compared to controls (10.4% vs. 6.7%; p<0.001). The MI patients showed higher frequency of T allele compared to the controls [0.33 vs. 0.22; OR (95% CI), 1.79 (1.37-2.34), p<0.001]. The association between rs2781666 G/T polymorphism of ARG I gene and MI remained significant after adjustment for other well-established risk factors.

CONCLUSION:

A significant association between rs2781666 G/T polymorphism of ARG I gene and MI was found in the Tunisian male population.

[Indexed for MEDLINE]
Icon for Elsevier Science
16.
Clin Biochem. 2009 Nov;42(16-17):1642-7. doi: 10.1016/j.clinbiochem.2009.08.019. Epub 2009 Sep 3.

Gender-specific effect of Pro12Ala polymorphism in peroxisome proliferator-activated receptor gamma-2 gene on obesity risk and leptin levels in a Tunisian population.

Author information

1
Research Laboratory LR99ES11, Biochemistry Department, Rabta University Hospital, Tunis, Tunisia.

Abstract

OBJECTIVES:

This study was undertaken to investigate the impact of the Pro12Ala (rs1801282) polymorphism of the peroxisome proliferator-activated receptor gamma-2 (PPARgamma-2) gene on obesity or body mass index (BMI) and plasma leptin, insulin, adiponectin and lipid levels in a sample of the Tunisian population.

DESIGN AND METHODS:

The study included 387 obese patients and 288 control subjects. The Pro12Ala genotype was determined by polymerase chain reaction followed by a digestion with the restriction of endonuclease BstUI.

RESULTS:

In the whole population, there is no significant difference in genotype frequencies of the Pro12Ala polymorphism between obese patients and controls. However, separate analysis by gender revealed that obese men (but not women) had significantly higher frequency of Pro/Ala genotypes compared to controls (12.2% vs. 4.1%; chi(2)=6.76, p=0.009). In comparison to Pro/Pro homozygotes, Ala-allele bearers had a significantly higher risk of obesity [OR (95% CI)=3.26 (1.28-8.33)]. When obese subjects were stratified according to type 2 diabetes status, the association with obesity was only significant in obese non-diabetic patients [OR (95% CI)=3.74 (1.43-9.74), p=0.007]. Additionally, obese male patients carrying the Ala-allele had significantly higher body mass index (p=0.007) and plasma leptin levels (p=0.023) compared to those homozygous for Pro-allele. The significant effect of Pro12Ala polymorphism on plasma leptin levels disappeared after adjustment for age and BMI.

CONCLUSION:

The present study provides evidence that the Pro12Ala polymorphism of the PPARgamma-2 gene is associated with obesity in non-diabetic men from Tunisian origin.

[Indexed for MEDLINE]
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17.
Arch Med Res. 2009 Apr;40(3):186-90. doi: 10.1016/j.arcmed.2009.02.008. Epub 2009 Apr 5.

LEPR p.Q223R Polymorphism influences plasma leptin levels and body mass index in Tunisian obese patients.

Author information

1
Biochemistry Department, Research Laboratory LR99ES11, Rabta University Hospital, Tunis, Tunisia.

Abstract

BACKGROUND AND AIMS:

The leptin receptor (LEPR) plays a crucial role in the regulation of body weight. Several common polymorphisms have been described in the human LEPR gene including the p.Q223R polymorphism (rs1137101). The association of this polymorphism with obesity or related metabolic phenotypes has been controversial. The aim of this study was to investigate the impact of the LEPR p.Q223R polymorphism on body mass index (BMI), plasma leptin and lipid parameters in a sample of the Tunisian population.

METHODS:

The study included 391 obese patients and 302 normal weight subjects. LEPR p.Q223R genotypes were identified by the PCR-RFLP analysis.

RESULTS:

Obese patients homozygous for RR genotype showed lower leptin levels than those with other genotypes (p = 0.005) adjusted for age, BMI and gender. Stratified analysis by gender revealed that obese male patients carrying the R allele showed significantly lower BMI (p = 0.007) and leptin levels (p = 0.037) than subjects homozygous for the Q allele. In obese women, the LEPR p.Q223R polymorphism was found associated with lower leptin concentrations (p = 0.05). After adjustment for age and BMI, the association between the LEPR variant and plasma leptin remained significant only within female patients (p = 0.027). A general linear model including leptin as dependant variable and age, BMI, menopausal status and genotype as covariates revealed that the LEPR p.Q223R polymorphism is independently associated with leptin levels in obese women (p = 0.026).

CONCLUSIONS:

Our findings suggest that the LEPR p.Q223R polymorphism influences plasma leptin levels and BMI in obese patients.

PMID:
19427969
DOI:
10.1016/j.arcmed.2009.02.008
[Indexed for MEDLINE]
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18.
Clin Biochem. 2009 Jun;42(9):852-6. doi: 10.1016/j.clinbiochem.2008.12.002. Epub 2008 Dec 14.

Association of a 27-bp repeat polymorphism in intron 4 of endothelial constitutive nitric oxide synthase gene with hypertension in a Tunisian population.

Author information

1
Research Laboratory LR99ES11, Department of Biochemistry, Rabta University Hospital, Tunis, Tunisia. jemaa_riadh@yahoo.fr

Abstract

OBJECTIVES:

Nitric oxide (NO) produced by endothelial nitric oxide synthase (eNOS) mediates endothelium-dependent vasodilatation and antithrombotic action. Controversial results regarding the association of eNOS gene (NOS3) polymorphisms with hypertension have been reported. In the present study, we examined a possible association between the 27-base pair (bp) repeat polymorphism in intron 4 of the NOS3 gene and hypertension in a sample of the Tunisian population.

DESIGN AND METHODS:

A total of 295 Tunisian patients with hypertension and 395 healthy controls were included in the study. The NOS3 gene intron 4a4b variable number of tandem repeats polymorphism was analyzed by PCR.

RESULTS:

A significant differences in genotype distribution and allele frequency was observed between patients and controls. Patients with hypertension had a frequency of 6.4% for the 4a4a genotype, 32.7% for the 4a4b genotype and 60.9% for the 4b4b genotype. The controls had a frequency of only 2.3% for the 4a4a genotype, 28.4% for the 4a4b genotype and 69.4% for the 4b4b genotype (chi(2)=11.81, p=0.003). The hypertension patient group showed a significant higher frequency of the 4a allele compared to the controls (0.23 vs. 0.16; chi(2)=8.61, p=0.003). The odds ratio of hypertension for 4a vs 4b allele frequencies was statistically significant 1.66 [1.09-2.53] at 95% CI, p=0.01 in males, whereas it was non-significant in females (1.23 [0.84-1.81], p=0.26).

CONCLUSION:

The present study showed a significant and independent association between the NOS34a4b gene polymorphism (presence of 4a allele) and hypertension in the Tunisian population.

[Indexed for MEDLINE]
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19.
Clin Biochem. 2009 May;42(7-8):584-8. doi: 10.1016/j.clinbiochem.2008.11.001. Epub 2008 Nov 13.

Association of G-2548A LEP polymorphism with plasma leptin levels in Tunisian obese patients.

Author information

1
Biochemistry Department, Research Laboratory LR99ES11, Rabta Hospital, Tunis, Tunisia.

Abstract

OBJECTIVES:

The aim of this study was to examine the association of the G-2548A polymorphism of the human leptin gene (LEP) with body mass index (BMI), plasma leptin, insulin, and lipid parameters in a sample of Tunisian population.

DESIGN AND METHODS:

Two hundred and twenty nine obese patients (BMI>or=30 kg/m(2)) were screened and compared to 251 normal weight subjects (BMI<25 kg/m(2)). The human leptin gene promoter G-2548A genotype was determined by polymerase chain reaction followed by a digestion with the restriction of endonuclease CfoI.

RESULTS:

In the entire study sample, carriers of -2548A allele had significantly lower leptin levels than homozygous for -2548G allele (14.28+/-9.10 ng/mL vs. 18.27+/-12 ng/mL, p<0.001 respectively) adjusted for BMI and gender. In obese patients but not control, subjects carrying the -2548A allele exhibited lower leptin levels than those with GG genotype (16.96+/-8.27 ng/mL vs. 21.37+/-11.72 ng/mL, p=0.001 respectively) adjusted for BMI and gender. In this group, carriership of the -2548A allele was identified, by multiple linear regression models, as significant independent predictor for leptin levels variability. Separate analyses by gender revealed that only in obese women, the -2548A allele was found to be associated with lower leptin levels independently of BMI (p=0.004).

CONCLUSIONS:

The present study showed that G-2548A LEP polymorphism is associated with lower leptin levels in Tunisian obese women.

[Indexed for MEDLINE]
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20.
Clin Biochem. 2009 Jan;42(1-2):34-7. doi: 10.1016/j.clinbiochem.2008.09.118. Epub 2008 Oct 18.

Association between the -2518G/A polymorphism in the monocyte chemoattractant protein-1 (MCP-1) gene and hypertension in Tunisian patients.

Author information

1
Research Laboratory LR99ES11, Department of Biochemistry, Rabta University Hospital, Tunis, Tunisia. jemaa_riadh@yahoo.fr

Abstract

OBJECTIVES:

Monocyte chemoattractant protein-1 (MCP-1:CCL2) has been demonstrated to be involved in the pathophysiology of atherosclerosis and hypertension. This study was aimed to investigate whether the single nucleotide polymorphism (SNP) at -2518 of the MCP-1 gene promoter region is associated to hypertension in a sample of Tunisian population.

DESIGN AND METHODS:

A total of 290 Tunisian patients with hypertension and 390 normotensive controls were included in the study. The SNP of the MCP-1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.

RESULTS:

A significant difference in genotype distribution and allele frequency was observed between patients and controls. Patients with hypertension had a frequency of 7.2% for the GG genotype, 35.2% for the AG genotype and 57.6% for the AA genotype. Normotensive subjects had a frequency of 3.6% for the GG genotype, 29.7% for the AG genotype and 66.7% for the AA genotype (chi(2)=8.02, p=0.01). The hypertension patient group showed a significant higher frequency of the G allele compared to the controls [0.24 vs. 0.18; OR (95%CI), 1.46 (1.11-1.91), p=0.004]. The association between the -2518 G/A polymorphism of MCP-1 gene and hypertension remained significant after adjustment for other well-established cardiovascular risk factors.

CONCLUSION:

The present study showed a significant and independent association between the -2518G/A polymorphism of the MCP-1 gene (presence of G allele) and hypertension in the Tunisian population.

[Indexed for MEDLINE]
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