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Biochem Mol Biol Int. 1996 Oct;40(2):253-61.

Subcellular redistribution of HSP72 protein during cisplatin-induced apoptosis in HeLa cells.

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División de Investigación Básica, Instituto Nacional de Cancerologia, Tlalpan, Mexico, D.F.


Exposure of HeLa cells to cisplatin results in the activation of apoptotic cell death. This drug induces DNA damage and generates reactive oxygen intermediates. Since cisplatin is highly reactive and binds to diverse proteins, it could create abnormal protein structures or nonspecific aggregates. For these reasons, we analyzed the expression and subcelullar distribution of hsp72, a heat-shock protein that enables cells under stress to cope with damaged proteins. We did not observe any changes in the expression of hsp72 protein, although, by immunofluorescence studies, we detected a dramatic redistribution of the protein. These results and its probable relevance in the drug-induced apoptotic phenomenon are discussed.

[Indexed for MEDLINE]

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