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Proc Natl Acad Sci U S A. 1988 Mar;85(5):1634-6.

Detection of human muscle glycogen by natural abundance 13C NMR.

Author information

1
Department of Molecular Biophysics, Yale University School of Medicine, New Haven, CT 06520.

Abstract

Natural abundance 13C nuclear magnetic resonance spectroscopy was used to detect signals from glycogen in the human gastrocnemius muscle. The reproducibility of the measurement was demonstrated, and the ability to detect dynamic changes was confirmed by measuring a decrease in muscle glycogen levels after exercise and its subsequent repletion. Single frequency gated 1H decoupling was used to obtain decoupled natural abundance 13C NMR spectra of the C-1 position of muscle glycogen.

PMID:
3422752
PMCID:
PMC279828
DOI:
10.1073/pnas.85.5.1634
[Indexed for MEDLINE]
Free PMC Article

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