Format

Send to

Choose Destination
Sci Adv. 2020 Jan 10;6(2):eaax4001. doi: 10.1126/sciadv.aax4001. eCollection 2020 Jan.

Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping.

Author information

1
Institute of Human Genetics, UMR 9002, CNRS-University of Montpellier, 34396 Montpellier, France.

Abstract

Polycomb repressive complexes 1 and 2 have been historically described as transcriptional repressors, but recent reports suggest that PRC1 might also support activation, although the underlying mechanisms remain elusive. Here, we show that stage-specific PRC1 binding at a subset of active promoters and enhancers during Drosophila development coincides with the formation of three-dimensional (3D) loops, an increase in expression during development and repression in PRC1 mutants. Dissection of the dachshund locus indicates that PRC1-anchored loops are versatile architectural platforms that persist when surrounding genes are transcriptionally active and fine-tune their expression. The analysis of RING1B binding profiles and 3D contacts during neural differentiation in mice suggests that this role is conserved in mammals.

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center