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Pathogens. 2019 Dec 31;9(1). pii: E36. doi: 10.3390/pathogens9010036.

Transcriptomic and Functional Studies of the RGS Protein Rax1 in Aspergillus fumigatus.

Author information

1
Department of Microbiology, Graduate School, Daejeon University, Daejeon 34520, Korea.
2
Soonchunhyang Institute of Medi-bio Science, Soonchunhyang University, Chungcheongnam-do 31151, Korea.
3
Department of Microbiology, Institute for Applied Biosciences, Karlsruhe Institute of Technology, Fritz-Haber Weg 4, D-76131 Karlsruhe, Germany.
4
Departments of Bacteriology and Genetics, University of Wisconsin-Madison, Madison, WI 53706, USA.
5
Department of Systems Biotechnology, Konkuk University, Seoul 05029, Korea.

Abstract

In the comparative transcriptomic studies of wild type (WT) and rax1 null mutant strains, we obtained an average of 22,222,727 reads of 101 bp per sample and found that 183 genes showed greater than 2.0-fold differential expression, where 92 and 91 genes were up-and down-regulated in rax1 compared to WT, respectively. In accordance with the significantly reduced levels of gliM and casB transcripts in the absence of rax1, the rax1 mutant exhibited increased sensitivity to exogenous gliotoxin (GT) without affecting levels of GT production. Moreover, rax1 resulted in significantly restricted colony growth and reduced viability under endoplasmic reticulum stress condition. In summary, Rax1 positively affects expression of gliM and metacaspase genes.

KEYWORDS:

Aspergillus fumigatus; GliM; RNA-seq; Rax1; metacaspase

PMID:
31906167
DOI:
10.3390/pathogens9010036
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