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Plant Cell. 2019 Aug 15. pii: tpc.00676.2018. doi: 10.1105/tpc.18.00676. [Epub ahead of print]

The Ectomycorrhizal Fungus Laccaria bicolor Produces Lipochitooligosaccharides and Uses the Common Symbiosis Pathway to Colonize Populus Roots.

Author information

1
South Dakota State University CITY: Brookings STATE: South Dakota POSTAL_CODE: 57007 United States Of America [US].
2
Université de Toulouse CITY: Toulouse France [FR].
3
University of Wisconsin - Madison CITY: Madison STATE: Wisconsin United States Of America [US].
4
University of Wisconsin, Platteville CITY: Platteville STATE: WI United States Of America [US].
5
North Carolina State University CITY: Raleigh STATE: NC United States Of America [US].
6
Oregon State University CITY: Corvallis STATE: OR United States Of America [US].
7
Oak Ridge National Laboratory CITY: Oak Ridge STATE: Tennessee United States Of America [US].
8
Oak Ridge National Laboratory CITY: Oak Ridge United States Of America [US].
9
Heidelberg University CITY: Heidelberg STATE: Baden-Württemberg Germany [DE].
10
South Dakota State University CITY: Brookings STATE: South Dakota POSTAL_CODE: 57006 United States Of America [US].
11
Oregon State University Department of Forest Science, Richardson Hall CITY: Corvallis STATE: Oregon POSTAL_CODE: 97331-5752 United States Of America [US].
12
University of Wisconsin - Madison CITY: Madison STATE: Wisconsin POSTAL_CODE: 53706 United States Of America [US] jeanmichel.ane@wisc.edu.

Abstract

Mycorrhizal fungi form mutualistic associations with the roots of most land plants and provide them with mineral nutrients from the soil in exchange for fixed carbon derived from photosynthesis. The common symbiosis pathway (CSP) is a conserved molecular signaling pathway in all plants capable of associating with arbuscular mycorrhizal fungi . It is required not only for arbuscular mycorrhizal symbiosis but also for rhizobia-legume and actinorhizal symbioses. Given its role in such diverse symbiotic associations, we hypothesized that the CSP also plays a role in ectomycorrhizal associations. We showed that the ectomycorrhizal fungus Laccaria bicolor produces an array of lipochitooligosaccharides (LCOs) that can trigger both root hair branching in legumes and, most importantly, calcium spiking in the host plant Populus in a CASTOR/POLLUX-dependent manner. Nonsulfated LCOs enhanced lateral root development in Populus in a CCaMK-dependent manner, and sulfated LCOs enhanced the colonization of Populus by L. bicolor. Compared to wild-type Populus, the colonization of CASTOR/POLLUX and CCaMK RNA interference lines by L. bicolor was reduced. Our work demonstrates that similar to other root symbioses, L. bicolor uses the CSP for the full establishment of its mutualistic association with Populus.

PMID:
31416823
DOI:
10.1105/tpc.18.00676
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