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Cold Spring Harb Perspect Biol. 2019 Aug 1;11(8). pii: a035386. doi: 10.1101/cshperspect.a035386.

CRISPR Tools for Systematic Studies of RNA Regulation.

Engreitz J#1,2, Abudayyeh O#1,3, Gootenberg J#1,4, Zhang F1,4,5,6,7.

Author information

1
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142.
2
Harvard Society of Fellows, Harvard University, Cambridge, Massachusetts 02139.
3
Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
4
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115.
5
McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
6
Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
7
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
#
Contributed equally

Abstract

SUMMARYRNA molecules perform diverse functions in mammalian cells, including transferring genetic information from DNA to protein and playing diverse regulatory roles through interactions with other cellular components. Here, we discuss how clustered regularly interspaced short palindromic repeat (CRISPR)-based technologies for directed perturbations of DNA and RNA are revealing new insights into RNA regulation. First, we review the fundamentals of CRISPR-Cas enzymes and functional genomics tools that leverage these systems. Second, we explore how these new perturbation technologies are transforming the study of regulation of and by RNA, focusing on the functions of DNA regulatory elements and long noncoding RNAs (lncRNAs). Third, we highlight an emerging class of RNA-targeting CRISPR-Cas enzymes that have the potential to catalyze studies of RNA biology by providing tools to directly perturb or measure RNA modifications and functions. Together, these tools enable systematic studies of RNA function and regulation in mammalian cells.

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