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Dev Cell. 2019 Aug 5;50(3):313-326.e5. doi: 10.1016/j.devcel.2019.07.007. Epub 2019 Jul 25.

Sidekick Is a Key Component of Tricellular Adherens Junctions that Acts to Resolve Cell Rearrangements.

Author information

1
Institut de Biologia Molecular de Barcelona, CSIC, Parc Científic de Barcelona, Baldiri Reixac, 10-12, Barcelona 08028, Spain.
2
Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA.
3
Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Parc Científic de Barcelona, Baldiri Reixac, 10, Barcelona 08028, Spain.
4
Department of Developmental, Molecular & Chemical Biology, Program in Cell, Molecular and Developmental Biology and Program in Genetics, Tufts University School of Medicine, 150 Harrison Avenue, Jaharis 322, Boston, MA 02111, USA.
5
Institut de Biologia Molecular de Barcelona, CSIC, Parc Científic de Barcelona, Baldiri Reixac, 10-12, Barcelona 08028, Spain. Electronic address: mlcbmc@ibmb.csic.es.
6
Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA. Electronic address: jessica.treisman@nyulangone.org.

Abstract

Tricellular adherens junctions are points of high tension that are central to the rearrangement of epithelial cells. However, the molecular composition of these junctions is unknown, making it difficult to assess their role in morphogenesis. Here, we show that Sidekick, an immunoglobulin family cell adhesion protein, is highly enriched at tricellular adherens junctions in Drosophila. This localization is modulated by tension, and Sidekick is itself necessary to maintain normal levels of cell bond tension. Loss of Sidekick causes defects in cell and junctional rearrangements in actively remodeling epithelial tissues like the retina and tracheal system. The adaptor proteins Polychaetoid and Canoe are enriched at tricellular adherens junctions in a Sidekick-dependent manner; Sidekick functionally interacts with both proteins and directly binds to Polychaetoid. We suggest that Polychaetoid and Canoe link Sidekick to the actin cytoskeleton to enable tricellular adherens junctions to maintain or transmit cell bond tension during epithelial cell rearrangements.

KEYWORDS:

Afadin; Sidekick; ZO-1; Zonula occludens; actin; cell rearrangements; epithelium; retina; tension; trachea; tricellular adherens junction

PMID:
31353315
PMCID:
PMC6748646
[Available on 2020-08-05]
DOI:
10.1016/j.devcel.2019.07.007

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