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Biomacromolecules. 2019 Jul 8;20(7):2625-2636. doi: 10.1021/acs.biomac.9b00417. Epub 2019 Jun 21.

Cross-Linked Elastin-like Polypeptide Membranes as a Model for Medial Arterial Calcification.

Author information

1
Department of Mining and Materials Engineering , McGill University , Montreal , Quebec H3A 0C5 , Canada.
2
Molecular Medicine , Hospital for Sick Children , Toronto , Ontario M5G 0A4 , Canada.
3
Department of Stomatology, Faculty of Dental Medicine , Université de Montréal , Montreal , Quebec H3C 3J7 , Canada.
4
Department of Biochemistry and Molecular Medicine , Faculty of Medicine, Université de Montréal , Montreal , Quebec H3C 3J7 , Canada.
5
Department of Biochemistry , University of Toronto , Toronto , Ontario M5S 1A8 , Canada.

Abstract

Calcium phosphate minerals deposit on the elastin-rich medial layers of arteries in the majority of seniors, diabetic, and chronic kidney disease patients, causing severe cardiovascular complications. There is no cure for medial calcification, and the mechanism of mineral formation on elastin layers is unknown. Here we propose cross-linked elastin-like polypeptide membranes as models to study medial calcification. Calcium phosphates deposit first on fibers and filaments and then spread to globular structures present in the membranes. Mineral phase evolution analyzed by near-edge X-ray spectroscopy matches that previously observed in a mouse model of medial calcification, showing that this simple system captures some of the key in vivo findings. This work shows how minerals form and evolve upon nucleation on elastin and provides an in vitro model that can be tuned to study hypotheses related to arterial calcification mechanisms and test drugs to stop or revert mineralization.

PMID:
31244014
DOI:
10.1021/acs.biomac.9b00417

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