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Annu Rev Biomed Eng. 2019 Mar 18. doi: 10.1146/annurev-bioeng-060418-052538. [Epub ahead of print]

Single-Cell Omics Analyses Enabled by Microchip Technologies.

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1
Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06511, USA; email: yanxiang.deng@yale.edu , amanda.finck@yale.edu , rong.fan@yale.edu.

Abstract

The study of single-cell omics provides unique information regarding the heterogeneity of cellular populations at various levels of the central dogma of molecular biology. This knowledge facilitates a deeper understanding of how underlying molecular and architectural changes alter cell behavior, development, and disease processes. The emerging microchip-based tools for single-cell omics analysis are enabling the evaluation of cellular omics with high throughput, improved sensitivity, and reduced cost. We review state-of-the-art microchip platforms for profiling genomics, epigenomics, transcriptomics, proteomics, metabolomics, and multi-omics at single-cell resolution. We also discuss the background of and challenges in the analysis of each molecular layer and integration of multiple levels of omics data, as well as how microchip-based methodologies benefit these fields. Additionally, we examine the advantages and limitations of these approaches. Looking forward, we describe additional challenges and future opportunities that will facilitate the improvement and broad adoption of single-cell omics in life science and medicine. Expected final online publication date for the Annual Review of Biomedical Engineering Volume 21 is June 4, 2019. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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