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Nat Commun. 2018 Dec 14;9(1):5313. doi: 10.1038/s41467-018-07662-4.

TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.

Author information

1
MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI, 48824, USA.
2
Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
3
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, 27695, USA.
4
Department of Molecular Genetics, The Ohio State University, Columbus, OH, 43210, USA.
5
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14850, USA.
6
Department of Plant Biochemistry, Georg-August-University, Albrecht-von-Haller-Institute for Plant Sciences, 37073, Göttingen, Germany.
7
MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI, 48824, USA. fb@msu.edu.
8
Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA. fb@msu.edu.

Abstract

Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.

PMID:
30552321
PMCID:
PMC6294250
DOI:
10.1038/s41467-018-07662-4
[Indexed for MEDLINE]
Free PMC Article

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