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Methods Mol Biol. 2019;1870:249-262. doi: 10.1007/978-1-4939-8808-2_19.

RNA Modification Regulatory Genes in DNA Damage.

Author information

1
Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
2
Department of Pathology, Yale University School of Medicine, New Haven, CT, USA. Narendra.Wajapeyee@yale.edu.

Abstract

Expression of genetic information is a multistep process which needs to be tightly regulated. One of the regulatory mechanisms is posttranscriptional modification of RNA, which can alter the stability, expression, or protein composition. Therefore, misregulation of this important cellular process can lead to pathological consequences, such as cancer development. It has been shown that alteration in the expression of certain RNA-modifying genes can promote tumorigenesis. Here, we present a mRNA expression analysis-based approach to comprehensively determine the expression of RNA readers/writers/erasers using DNA damage as an example, and then to validate the effect of altered RNA reader/writer/erasers in regulating the DNA damage response.

KEYWORDS:

DNA damage; Gene expression analysis; RNA modification

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