Format

Send to

Choose Destination
Viruses. 2018 Nov 27;10(12). pii: E668. doi: 10.3390/v10120668.

Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages.

Author information

1
School of Microbiology & APC Microbiome Ireland, University College Cork, Western Road, Cork T12 YT20, Ireland. stephen.hayes@umail.ucc.ie.
2
Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Campus de Luminy, 13288 Marseille, France. Yoan.Duhoo@afmb.univ-mrs.fr.
3
Department of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, Germany. horst.neve@mri.bund.de.
4
School of Microbiology & APC Microbiome Ireland, University College Cork, Western Road, Cork T12 YT20, Ireland. james.murphy@umail.ucc.ie.
5
Biomedical Research Institute, Hasselt University, 3500 Diepenbeek, Belgium. jeanpaul.noben@uhasselt.be.
6
Department of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, Germany. charles.franz@mri.bund.de.
7
Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Campus de Luminy, 13288 Marseille, France. cambillau@afmb.univ-mrs.fr.
8
Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, Campus de Luminy, 13288 Marseille, France. cambillau@afmb.univ-mrs.fr.
9
School of Microbiology & APC Microbiome Ireland, University College Cork, Western Road, Cork T12 YT20, Ireland. j.mahony@ucc.ie.
10
FrieslandCampina, 3818 Amersfoort, The Netherlands. arjen.nauta@frieslandcampina.com.
11
School of Microbiology & APC Microbiome Ireland, University College Cork, Western Road, Cork T12 YT20, Ireland. d.vansinderen@ucc.ie.

Abstract

Siphoviridae of the lactococcal 936 group are the most commonly encountered bacteriophages in the dairy processing environment. The 936 group phages possess a discrete baseplate at the tip of their tail-a complex harbouring the Receptor Binding Protein (RBP) which is responsible for host recognition and attachment. The baseplate-encoding region is highly conserved amongst 936 phages, with 112 of 115 publicly available phages exhibiting complete synteny. Here, we detail the three exceptions (Phi4.2, Phi4R15L, and Phi4R16L), which differ from this genomic architecture in possessing an apparent second RBP-encoding gene upstream of the "classical" rbp gene. The newly identified RBP possesses an elongated neck region relative to currently defined 936 phage RBPs and is genetically distinct from defined 936 group RBPs. Through detailed characterisation of the representative phage Phi4.2 using a wide range of complementary techniques, we demonstrated that the above-mentioned three phages possess a complex and atypical baseplate structure. Furthermore, the presence of both RBPs in the tail tip of the mature virion was confirmed, while the anticipated host-binding capabilities of both proteins were also verified.

KEYWORDS:

host interactions; lactic acid bacteria; structure; virus

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center