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J Periodontol. 2019 Mar;90(3):295-305. doi: 10.1002/JPER.18-0226. Epub 2018 Oct 7.

Periodontitis causes abnormalities in the liver of rats.

Author information

1
Medicinal Plants Research Center (NPPM), Federal University of Piauí, Teresina, PI, Brazil.
2
Medicine School, Education Institute of Parnaiba Valley (IESVAP), Parnaíba, PI, Brazil.
3
Laboratory of Histological Analysis and Preparation (LAPHIS), Federal University of Piauí, Parnaíba, PI, Brazil.
4
Piracicaba Dental School, State University of Campinas - UNICAMP, Department of Morphology, Division of Histology, Piracicaba, SP, Brazil.
5
Laboratory of Experimental Physiopharmacology (LAFFEX), Federal University of Piauí, Parnaíba, PI, Brazil.
6
Yale Cardiovascular Research Center, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA.

Abstract

BACKGROUND:

Periodontitis not only causes injury to the periodontium, but also damages other tissues such as: articulate, renal, cardiac, and hepatic. The objective of this study was to investigate periodontitis induced alterations in liver function and structure using an experimental model.

METHODS:

Twenty female rats (Rattus norvegicus) were allocated into two groups: control and periodontitis. Gingival bleeding index and oxidative stress parameters and specific circulating biomarkers were measured. Immunohistochemistry was carried out using alkaline phosphatase (AlkP) staining of the liver. Hepatic tissues, cytokines, and lipid contents were measured. Histopathologic evaluation of the liver was carried out using light and electron microscopy.

RESULTS:

Liver histopathologic and immunohistochemistry assessment showed increase in steatosis score, and presence of binucleate hepatocytes and positive cells for AlkP in periodontitis versus control group. Ultrastructural evaluation showed significant increase in size and number of lipid droplets (LD), distance between the cisterns of rough endoplasmic reticulum (RER), mitochondria size, foamy cytoplasm, and glycogen accumulation in the liver of the periodontitis group compared with the control group. In addition, plasma levels of AlkP, high-density lipoprotein (HDL), triglycerides, and total cholesterol were also changed.

CONCLUSION:

Experimental periodontitis caused immunohistochemistry, histopathologic, ultrastructural, oxidative, and biochemical changes in the liver of rats.

KEYWORDS:

cytokines; inflammation; liver; oral medicine; periodontal diseases

PMID:
30216457
DOI:
10.1002/JPER.18-0226

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