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Genetics. 2018 Oct;210(2):653-663. doi: 10.1534/genetics.118.301444. Epub 2018 Aug 6.

Blimp-1 Mediates Tracheal Lumen Maturation in Drosophila melanogaster.

Author information

1
Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Cientific de Barcelona, C. Baldiri Reixac 10, 08028 Barcelona, Spain.
2
Institut de Recerca Biomedica de Barcelona (IRB Barcelona), The Barcelona Institute of Science and Technology, C. Baldiri Reixac 10, 08028 Barcelona, Spain, and.
3
Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Parc Cientific de Barcelona, C. Baldiri Reixac 10, 08028 Barcelona, Spain sofiajaraujo@ub.edu jcrbmc@ibmb.csic.es.
4
Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain and Institute of Biomedicine University of Barcelona (IBUB).

Abstract

The specification of tissue identity during embryonic development requires precise spatio-temporal coordination of gene expression. Many transcription factors required for the development of organs have been identified and their expression patterns are known; however, the mechanisms through which they coordinate gene expression in time remain poorly understood. Here, we show that hormone-induced transcription factor Blimp-1 participates in the temporal coordination of tubulogenesis in Drosophila melanogaster by regulating the expression of many genes involved in tube maturation. In particular, we demonstrate that Blimp-1 regulates the expression of genes involved in chitin deposition and F-actin organization. We show that Blimp-1 is involved in the temporal control of lumen maturation by regulating the beginning of chitin deposition. We also report that Blimp-1 represses a variety of genes involved in tracheal maturation. Finally, we reveal that the kinase Btk29A serves as a link between Blimp-1 transcriptional repression and apical extracellular matrix organization.

KEYWORDS:

Blimp-1; Btk29A; Drosophila; aECM; chitin; trachea

PMID:
30082278
PMCID:
PMC6216578
[Available on 2019-10-01]
DOI:
10.1534/genetics.118.301444
[Indexed for MEDLINE]

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