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Hum Cell. 2018 Oct;31(4):292-299. doi: 10.1007/s13577-018-0214-1. Epub 2018 Jun 15.

Circulating miR-215-5p and miR-642a-5p as potential biomarker for diagnosis of osteosarcoma in Mexican population.

Author information

1
Department of Genetics, National Institute of Rehabilitation, Calzada Mexico-Xochimilco 289, Arenal de Guadalupe, 14389, Mexico City, Mexico.
2
Genomics of Bone Metabolism Laboratory, National Institute of Genomic Medicine (INMEGEN), Periferico Sur 4809, Arenal Tepepan, 14610, Mexico City, Mexico.
3
CONACYT-National Institute of Genomic Medicine (INMEGEN), Periferico Sur 4809, Arenal Tepepan, 14610, Mexico City, Mexico.
4
Genomics of Bone Metabolism Laboratory, National Institute of Genomic Medicine (INMEGEN), Periferico Sur 4809, Arenal Tepepan, 14610, Mexico City, Mexico. rvelazquez@inmegen.gob.mx.
5
Department of Genetics, National Institute of Rehabilitation, Calzada Mexico-Xochimilco 289, Arenal de Guadalupe, 14389, Mexico City, Mexico. dr_genetica@yahoo.com.

Abstract

Osteosarcoma is the most common malignant bone neoplasia affecting individuals in the second decade of life. The survival rate has not been improved during the last 25 years, in part because of the lack of specific markers. The microRNAs have been identified as important regulators of gene expression, experimental evidence suggests these molecules as key players in cancer development and progression. To identify miRNAs differentially expressed in serum from patients with osteosarcoma compared to healthy donors in Mexican population. Fifteen osteosarcoma patients and fifteen age and sex matched healthy individuals were recruited. Two pools of total RNA extracted from serum per study group were prepared and the miRNA expression profiles were analyzed through TaqMan Low Density Arrays. Validation was carried out through RT-qPCR using individual TaqMan assays for those miRNAs differentially expressed. Fifteen miRNAs were differentially expressed in osteosarcoma patients compared to healthy controls. Overexpression of miR-215-5p and miR-642a-5p was confirmed by validation through RT-qPCR. The expression analysis of miRNAs from serum in osteosarcoma patients revealed differential expression of miR-215-5p and miR-642a-5p. Both microRNAs are potential markers for osteosarcoma diagnosis.

KEYWORDS:

Biomarkers; Osteosarcoma; Serum; Target genes; microRNAs

PMID:
29907935
DOI:
10.1007/s13577-018-0214-1
[Indexed for MEDLINE]

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